Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,886,465	T→C	Y464H (TAC→CAC)	yidC →	membrane protein insertase YidC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,886,465	0	T	C	100.0%	260.3 / NA	74	Y464H (TAC→CAC)	yidC	membrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/36);  total (38/36)

CCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGG  >  NC_000913/3886329‑3886599                                                                                                                                         |                                                                                                                                       cccGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGcact                                                                                                                                     <  1:589278/140‑1 (MQ=255) cccGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGcact                                                                                                                                     <  2:107225/140‑1 (MQ=255)      tGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATc                                                                                                                                >  1:402211/1‑140 (MQ=255)      tGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATc                                                                                                                                >  2:785703/1‑140 (MQ=255)       gggCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATcc                                                                                                                               >  1:488873/1‑140 (MQ=255)          cggcTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGTACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGc                                                                                                                            <  2:857133/140‑1 (MQ=255)          cggcTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGc                                                                                                                            <  2:87040/140‑1 (MQ=255)          cggcTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGc                                                                                                                            <  1:261722/140‑1 (MQ=255)            gctgctTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCg                                                                                                                          >  2:91924/1‑140 (MQ=255)                    ccGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGAt                                                                                                                  <  2:488873/140‑1 (MQ=255)                    ccGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGAt                                                                                                                  <  2:656986/140‑1 (MQ=255)                    ccGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGAt                                                                                                                  <  1:536632/140‑1 (MQ=255)                       ctgctgATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATggg                                                                                                               <  1:802297/140‑1 (MQ=255)                          ctgATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATggg                                                                                                               <  2:384965/137‑1 (MQ=255)                          ctgATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATggg                                                                                                               >  1:384965/1‑137 (MQ=255)                            gATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTaa                                                                                                          <  2:654348/140‑1 (MQ=255)                            gATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTaa                                                                                                          <  1:562405/140‑1 (MQ=255)                                    tGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGttc                                                                                                  >  2:132889/1‑140 (MQ=255)                                    tGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGttc                                                                                                  >  1:164226/1‑140 (MQ=255)                                     gCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGttct                                                                                                 <  2:402211/140‑1 (MQ=255)                                      ccAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGttctt                                                                                                >  1:84685/1‑140 (MQ=255)                                            ttCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCttcattca                                                                                          <  2:84685/140‑1 (MQ=255)                                                      tGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCg                                                                                <  1:785703/140‑1 (MQ=255)                                                      tGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCg                                                                                >  2:139303/1‑140 (MQ=255)                                                       gTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCg                                                                                <  1:28997/139‑1 (MQ=255)                                                       gTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCg                                                                                >  2:28997/1‑139 (MQ=255)                                                        tactacATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGcc                                                                              >  2:22951/1‑140 (MQ=255)                                                          ctacATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGa                                                                            <  1:132889/140‑1 (MQ=255)                                                                gCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAg                                                                      >  2:709072/1‑140 (MQ=255)                                                                gCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCAATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAg                                                                      >  2:564266/1‑140 (MQ=255)                                                                  tGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTg                                                                    >  2:254990/1‑140 (MQ=255)                                                                  tGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTg                                                                    >  2:547160/1‑140 (MQ=255)                                                                  tGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTg                                                                    >  1:270427/1‑140 (MQ=255)                                                                    aTGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgac                                                                  >  1:788135/1‑140 (MQ=255)                                                                    aTGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgac                                                                  <  2:45596/140‑1 (MQ=255)                                                                    aTGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgac                                                                  <  2:433536/140‑1 (MQ=255)                                                                     tGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgacc                                                                 >  1:238799/1‑140 (MQ=255)                                                                       ggTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgaccga                                                               >  2:719834/1‑140 (MQ=255)                                                                       ggTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgaccga                                                               <  1:547160/140‑1 (MQ=255)                                                                       ggTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgaccga                                                               <  1:254990/140‑1 (MQ=255)                                                                          tCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGAccc                                                            >  1:780822/1‑140 (MQ=255)                                                                            cGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgac                                                                  <  1:260537/132‑1 (MQ=255)                                                                            cGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgac                                                                  >  2:260537/1‑132 (MQ=255)                                                                              ttGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATg                                                        <  2:700655/140‑1 (MQ=255)                                                                                   cTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATgcagca                                                   >  2:850992/1‑140 (MQ=255)                                                                                     gCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCaga                                                 <  1:719834/140‑1 (MQ=255)                                                                                     gCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCaga                                                 <  2:164226/140‑1 (MQ=255)                                                                                      cGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgaccga                                                               >  2:374897/1‑125 (MQ=255)                                                                                      cGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgaccga                                                               <  1:374897/125‑1 (MQ=255)                                                                                      cGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCagaa                                                >  2:48796/1‑140 (MQ=255)                                                                                      cGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCagaa                                                >  2:614877/1‑140 (MQ=255)                                                                                        tCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCagaaga                                              >  1:804111/1‑140 (MQ=255)                                                                                            gCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCAt                                          <  1:850992/140‑1 (MQ=255)                                                                                                cGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATgcag                                                     >  1:344832/1‑125 (MQ=255)                                                                                                cGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATgcag                                                     <  2:344832/125‑1 (MQ=255)                                                                                                cGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGAcc                                      >  2:375232/1‑140 (MQ=255)                                                                                                cGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGAcc                                      >  1:52671/1‑140 (MQ=255)                                                                                                  tttGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATgcag                                                     <  1:702888/123‑1 (MQ=255)                                                                                                  tttGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATgcag                                                     >  2:702888/1‑123 (MQ=255)                                                                                                      cACTGTGGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATg                                >  2:66269/1‑140 (MQ=255)                                                                                                          gtgGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCgg                            >  2:567020/1‑140 (MQ=255)                                                                                                           tgGATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGt                           <  2:834942/140‑1 (MQ=255)                                                                                                             gATCCACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCa                         <  1:48796/140‑1 (MQ=255)                                                                                                                ccACGACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCt                      <  1:139303/140‑1 (MQ=255)                                                                                                                    gACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCAc                  <  2:270427/140‑1 (MQ=255)                                                                                                                    gACCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCAc                  <  1:709072/140‑1 (MQ=255)                                                                                                                     aCCTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCAcc                 >  1:103511/1‑140 (MQ=255)                                                                                                                      ccTGTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCg                <  1:375232/140‑1 (MQ=255)                                                                                                                         gTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCgtgt             <  2:788135/140‑1 (MQ=255)                                                                                                                         gTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCg                >  1:749903/1‑137 (MQ=255)                                                                                                                         gTCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCg                <  2:749903/137‑1 (MQ=255)                                                                                                                          tCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGtt            >  1:21373/1‑140 (MQ=255)                                                                                                                          tCGGCACAGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGtt            >  1:655563/1‑140 (MQ=255)                                                                                                                                 aGGACCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCtg     <  1:101465/140‑1 (MQ=255)                                                                                                                                    aCCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTgg  >  2:800455/1‑140 (MQ=255)                                                                                                                                    aCCCGCACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTgg  >  2:452848/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGG  >  NC_000913/3886329‑3886599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑