Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,101,899 A→G V70V (GTA→GTG)  kdgT → 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,101,8990AG100.0% 148.2 / NA 45V70V (GTA→GTG) kdgT2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/24);  total (21/24)

CTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGC  >  NC_000913/4101770‑4102027
                                                                                                                                 |                                                                                                                                
cTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAAt                                                                                                                        >  2:586635/1‑140 (MQ=255)
      gCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTa                                                                                                                  <  2:150983/140‑1 (MQ=255)
         gggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGCACGc                                                                                                               <  1:337091/140‑1 (MQ=255)
          ggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCt                                                                                                              >  2:640085/1‑140 (MQ=255)
          ggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCt                                                                                                              >  1:214647/1‑140 (MQ=255)
           gggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtg                                                                                                             <  1:586635/140‑1 (MQ=255)
              cGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtggtg                                                                                                          <  2:245194/140‑1 (MQ=255)
                 ggAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCAGTACGCTGGTGGTa                                                                                                       <  1:762368/140‑1 (MQ=255)
                                  ttCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgc                                                                                      >  1:225049/1‑140 (MQ=255)
                                  ttCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgc                                                                                      >  2:151382/1‑140 (MQ=255)
                                     aCCAACGNGATNNTTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtg                                                                                   >  2:321859/1‑140 (MQ=255)
                                     aCCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtg                                                                                   >  1:675596/1‑140 (MQ=255)
                                       cAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtggg                                                                                 <  2:214647/140‑1 (MQ=255)
                                       cAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtggg                                                                                 >  1:510664/1‑140 (MQ=255)
                                          caggATGATTACCGGTACGGTACCAATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtgggagg                                                                              >  2:148655/3‑140 (MQ=255)
                                          cGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtgggtgg                                                                              >  2:458911/1‑140 (MQ=255)
                                            ggATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGtt                                                                            >  2:430240/1‑140 (MQ=255)
                                              atgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGc                                                                          <  1:430240/140‑1 (MQ=255)
                                              atgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGc                                                                          <  2:225049/140‑1 (MQ=255)
                                                 atTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGc                                                                       >  1:192901/1‑140 (MQ=255)
                                                     ccGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAAtt                                                                   >  1:841510/1‑140 (MQ=255)
                                                      cGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTg                                                                  <  1:656301/140‑1 (MQ=255)
                                                        gTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtgg                                                                                  <  2:176884/122‑1 (MQ=255)
                                                        gTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtgg                                                                                  >  1:176884/1‑122 (MQ=255)
                                                         tACGGTGCCCATTCTGGCGGTGTGG‑TTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTc                                                              <  2:707614/140‑1 (MQ=255)
                                                          aCGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTc                                                              <  2:749603/140‑1 (MQ=255)
                                                           cGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGc                                                                 >  1:738854/1‑136 (MQ=255)
                                                           cGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGc                                                                 <  2:738854/136‑1 (MQ=255)
                                                             gTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTcgcg                                                           <  1:640085/140‑1 (MQ=255)
                                                               gCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGta                                                         <  1:321859/140‑1 (MQ=255)
                                                                cccATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtat                                                        <  1:717885/140‑1 (MQ=255)
                                                                cccATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtat                                                        >  2:717885/1‑140 (MQ=255)
                                                                cccATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtat                                                        >  1:375463/1‑140 (MQ=255)
                                                                     tCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTc                                                   <  2:675596/140‑1 (MQ=255)
                                                                            gtgtGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACa                                            <  2:68756/140‑1 (MQ=255)
                                                                             tgtgGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGta                                                         <  1:344635/126‑1 (MQ=255)
                                                                             tgtgGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGta                                                         >  2:344635/1‑126 (MQ=255)
                                                                               tgGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGaa                                              >  2:225304/1‑135 (MQ=255)
                                                                               tgGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGaa                                              <  1:225304/135‑1 (MQ=255)
                                                                                    tttttGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTg                                    <  2:569505/140‑1 (MQ=255)
                                                                                       ttGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAg                                 >  1:211928/1‑140 (MQ=255)
                                                                                              ggggCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGAtt                          <  2:510664/140‑1 (MQ=255)
                                                                                                      aaTAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCg                  <  1:741516/140‑1 (MQ=255)
                                                                                                                aGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAc        >  1:275598/1‑140 (MQ=255)
                                                                                                                  cgcgACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAcgc      <  1:151382/140‑1 (MQ=255)
                                                                                                                      aCGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAcgctggc  >  2:548339/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                
CTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGC  >  NC_000913/4101770‑4102027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑