Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	379,399	A→G	V58A (GTG→GCG)	frmA ←	S‑(hydroxymethyl)glutathione dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	379,399	0	A	G	100.0%	153.1 / NA	44	V58A (GTG→GCG)	frmA	S‑(hydroxymethyl)glutathione dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/25);  total (19/25)

TTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCG  >  NC_000913/379265‑379531                                                                                                                                       |                                                                                                                                     tttgGCCAGAACGACAGAACTCACCCCCGCCGCCCTCCGCGGTGTAAAGCGGGATCACATGGTCGCCGGGTTTGACGCTGGTTACGCTTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCggg                                                                                                                                 <  2:394068/140‑1 (MQ=255)     gCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATa                                                                                                                             >  1:459263/1‑140 (MQ=255)       cAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATaca                                                                                                                           <  1:538064/140‑1 (MQ=255)                 gAACTCACACTCGCCGCCCTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGt                                                                                                                 <  2:756107/140‑1 (MQ=255)                 gAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGt                                                                                                                 <  1:462496/140‑1 (MQ=255)                     tCACACTCGCCGCCCTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATc                                                                                                             <  2:280116/140‑1 (MQ=255)                       acacTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGc                                                                                                           >  2:358345/1‑140 (MQ=255)                         ccccgccgcCCTCCGCGGTGTAAAGCGGGATCACATGGTCGCCGGTTTTGCCGCTGGTTACGCCTTCCCCGCCTTCACCCACACCGCCGGCCCCTTCGTGCCCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCg                                                                                                         <  2:202496/137‑1 (MQ=255)                         acTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCg                                                                                                         <  2:194653/140‑1 (MQ=255)                            cgccgcACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGgag                                                                                                      >  2:584049/1‑140 (MQ=255)                            cgccgcACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGgag                                                                                                      >  2:238838/1‑140 (MQ=255)                             gccgcACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGgaga                                                                                                     >  1:219173/1‑140 (MQ=255)                              ccgcACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGagag                                                                                                    >  1:243539/1‑140 (MQ=255)                               cgcACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAgg                                                                                                   >  1:219402/1‑140 (MQ=255)                                    tCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCCCCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTaaa                                                                                              <  1:20166/140‑1 (MQ=255)                                         ggTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGt                                                                                         <  1:358345/140‑1 (MQ=255)                                                  cGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGc                                                                                >  1:492798/1‑140 (MQ=255)                                                  cGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGc                                                                                >  1:83396/1‑140 (MQ=255)                                                   gggATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCa                                                                               <  1:238838/140‑1 (MQ=255)                                                   gggATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCa                                                                               <  1:584049/140‑1 (MQ=255)                                                   gggATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCa                                                                               <  1:812724/140‑1 (MQ=255)                                                       tCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACg                                                                           >  1:119562/1‑140 (MQ=255)                                                        cacaTGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGc                                                                          <  1:409890/140‑1 (MQ=255)                                                               tCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCa                                                                               <  1:278127/128‑1 (MQ=255)                                                               tCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCa                                                                               >  2:278127/1‑128 (MQ=255)                                                               tCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATg                                                                   >  1:415283/1‑140 (MQ=255)                                                               tCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATg                                                                   >  2:339083/1‑140 (MQ=255)                                                               tCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATg                                                                   >  2:418334/1‑140 (MQ=255)                                                                 gCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATggg                                                                 <  2:415283/140‑1 (MQ=255)                                                                 cccAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATggg                                                                 <  2:528082/139‑1 (MQ=255)                                                                    aGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGa                                                              <  2:459263/140‑1 (MQ=255)                                                                      gTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACt                                                            <  2:83396/140‑1 (MQ=255)                                                                      gTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACt                                                            <  2:492798/140‑1 (MQ=255)                                                                         tGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATggg                                                                 <  2:329765/132‑1 (MQ=255)                                                                         tGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATggg                                                                 >  1:329765/1‑132 (MQ=255)                                                                              cTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAg                                                    <  1:445793/140‑1 (MQ=255)                                                                                           cACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCtttt                                       >  2:9505/1‑140 (MQ=255)                                                                                             ccGATTCCACCCACACCCCCGGCCCTTTCGTGCCGGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCtttttt                                     <  2:219402/140‑1 (MQ=255)                                                                                             ccGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCtttttt                                     <  1:433752/140‑1 (MQ=255)                                                                                                 cTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCTTTTTTCggt                                 <  2:709045/140‑1 (MQ=255)                                                                                                    cAACCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCTTTTTTCggtggt                              >  1:783723/1‑140 (MQ=255)                                                                                                      aCCACAACGCCGGCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCTTTTTTCGGTGGTGc                            <  1:339083/140‑1 (MQ=255)                                                                                                                  gCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCa                           <  1:101835/129‑1 (MQ=255)                                                                                                                  gCCCCTTCGTGACCGAGAACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCa                           >  2:101835/1‑129 (MQ=255)                                                                                                                                gagaACCGCCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCg  >  1:778505/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     TTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCGGGTCATCGCCGGAGAGGGTAAATGCGTCGGTATGGCAAACGCCGGTATGGGTGACTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCG  >  NC_000913/379265‑379531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑