Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,453,756	A→G	S58S (TCA→TCG)	yjfF →	galactofuranose ABC transporter putative membrane subunit YjtF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,453,756	0	A	G	100.0%	196.4 / NA	56	S58S (TCA→TCG)	yjfF	galactofuranose ABC transporter putative membrane subunit YjtF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/23);  total (33/23)

CGTCTTTGTGTTGGGTTATCTTTACTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGC  >  NC_000913/4453621‑4453890                                                                                                                                        |                                                                                                                                       cGTCTTTGTGTTGGGTTATCTTTACTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTg                                                                                                                                    <  2:556082/140‑1 (MQ=255)  gTCTTTGTGTTGGGTTATCTTTACTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTgg                                                                                                                                   >  1:96387/1‑140 (MQ=255)   tCTTTGTGTTGGGTTATCTTTACTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTggg                                                                                                                                  >  1:137364/1‑140 (MQ=255)   tCTTTGTGTTGGGTTATCTTTACTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTATGATCCTCTCGGGTggg                                                                                                                                  >  1:314285/1‑140 (MQ=255)      ttGTGTTGGGTTATCTTTACTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGgatc                                                                                                                               >  1:739814/1‑140 (MQ=255)         tgtTGGGTTATCTTTACTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGgatcgat                                                                                                                            <  1:293224/140‑1 (MQ=255)                   tCTTTACTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGtcg                                                                                                                  >  2:319119/1‑140 (MQ=255)                        aCTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGtcggttcg                                                                                                             >  1:518121/1‑140 (MQ=255)                           gCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTg                                                                                                          >  1:528790/1‑140 (MQ=255)                            ccTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGtcggttc                                                                                                              >  2:466158/1‑135 (MQ=255)                            ccTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGtcggttc                                                                                                              <  1:466158/135‑1 (MQ=255)                            ccTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGa                                                                                                         <  2:518121/140‑1 (MQ=255)                             cTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTg                                                                                                          <  1:18704/138‑1 (MQ=255)                             cTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTg                                                                                                          >  2:18704/1‑138 (MQ=255)                              tGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATc                                                                                                       >  2:629966/1‑140 (MQ=255)                                                         caAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTATGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCaaaa                                                                            <  2:314285/140‑1 (MQ=255)                                                                 aTCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTgg                                                                    >  1:235123/1‑140 (MQ=255)                                                                      cAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGAtt                                                               >  2:434610/1‑140 (MQ=255)                                                                      cAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGAtt                                                               >  2:460647/1‑140 (MQ=255)                                                                      cAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGAtt                                                               >  2:98771/1‑140 (MQ=255)                                                                       aaTATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGAttt                                                              >  1:696611/1‑140 (MQ=255)                                                                       aaTATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGAttt                                                              <  1:686037/140‑1 (MQ=255)                                                                           tCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGc                                                          <  1:697474/140‑1 (MQ=255)                                                                           tCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGc                                                          >  2:697474/1‑140 (MQ=255)                                                                           tCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGc                                                          >  2:404863/1‑140 (MQ=255)                                                                           tCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGc                                                          >  2:381854/1‑140 (MQ=255)                                                                              tGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCctc                                                       >  1:704316/1‑140 (MQ=255)                                                                                aCCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCtctc                                                     >  1:210248/1‑140 (MQ=255)                                                                                aCCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCtctc                                                     >  1:261858/1‑140 (MQ=255)                                                                                aCCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCtctc                                                     >  2:303019/1‑140 (MQ=255)                                                                                aCCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCtctc                                                     >  1:603390/1‑140 (MQ=255)                                                                                aCCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCtctc                                                     >  2:365857/1‑140 (MQ=255)                                                                                  cGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTcgc                                                   <  1:460647/140‑1 (MQ=255)                                                                                            tttCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGc                                         <  1:365857/140‑1 (MQ=255)                                                                                              tCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGt                                       >  1:830524/1‑140 (MQ=255)                                                                                               cTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGtt                                      >  2:404160/1‑140 (MQ=255)                                                                                                    gATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGc                                 <  1:434610/140‑1 (MQ=255)                                                                                                    gATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGc                                 <  2:792521/140‑1 (MQ=255)                                                                                                     aTCATTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCt                                <  2:696611/140‑1 (MQ=255)                                                                                                        aTTGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGt                             >  1:732586/1‑140 (MQ=255)                                                                                                          tGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGc                           <  2:96387/140‑1 (MQ=255)                                                                                                          tGCCGTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGc                           <  1:404160/140‑1 (MQ=255)                                                                                                              gTTGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGt                       >  2:692503/1‑140 (MQ=255)                                                                                                               ttGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTg                      >  2:48050/1‑140 (MQ=255)                                                                                                               ttGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTg                      <  2:114391/140‑1 (MQ=255)                                                                                                                tGGCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGa                     <  2:235123/140‑1 (MQ=255)                                                                                                                 ggCATGACCTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTACTGGCGTTTCCGCTAGTGCTGGTGAt                    >  2:191819/1‑140 (MQ=255)                                                                                                                        ccTTTGTGATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCtgt             <  2:732586/140‑1 (MQ=255)                                                                                                                            tgtgATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGc                           <  1:562574/122‑1 (MQ=255)                                                                                                                            tgtgATCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGc                           >  2:562574/1‑122 (MQ=255)                                                                                                                                aTCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCgg     <  1:629966/140‑1 (MQ=255)                                                                                                                                aTCCTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCgg     <  2:830524/140‑1 (MQ=255)                                                                                                                                  ccTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGgcg   <  1:692503/140‑1 (MQ=255)                                                                                                                                  ccTCTCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGgcg   <  2:704316/140‑1 (MQ=255)                                                                                                                                  ccTCTCGGGT‑GGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGgcgc  >  1:478599/1‑140 (MQ=255)                                                                                                                                   ctctCGGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGgcgc  >  1:758465/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CGTCTTTGTGTTGGGTTATCTTTACTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGC  >  NC_000913/4453621‑4453890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑