Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	396,598	A→G	intergenic (‑311/‑41)	ampH ← / → sbmA	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase/peptide antibiotic/peptide nucleic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	396,598	0	A	G	100.0%	173.2 / NA	50	intergenic (‑311/‑41)	ampH/sbmA	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase/peptide antibiotic/peptide nucleic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/27);  total (23/27)

TTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGG  >  NC_000913/396461‑396733                                                                                                                                          |                                                                                                                                        ttATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATAcgca                                                                                                                                       >  2:139146/1‑140 (MQ=255)  tATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGcag                                                                                                                                      <  1:139146/140‑1 (MQ=255)  tATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGcag                                                                                                                                      <  1:490040/140‑1 (MQ=255)   aTGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGcagc                                                                                                                                     <  1:179435/140‑1 (MQ=255)             tCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTaaa                                                                                                                           >  1:107918/1‑140 (MQ=255)             tCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTaaa                                                                                                                           <  2:107918/140‑1 (MQ=255)             tCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTaaa                                                                                                                           <  1:818818/140‑1 (MQ=255)             tCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTaaa                                                                                                                           <  1:568310/140‑1 (MQ=255)               tttGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAAc                                                                                                                         >  1:531818/1‑140 (MQ=255)                  ggTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGAt                                                                                                                      <  2:50756/140‑1 (MQ=255)                    tttGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATaa                                                                                                                    >  1:184252/1‑140 (MQ=255)                     ttGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATaag                                                                                                                   <  2:550710/140‑1 (MQ=255)                       gtgAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACACTGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATaagaa                                                                                                                 >  2:796543/1‑140 (MQ=255)                       gtgAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATaagaa                                                                                                                 >  1:820252/1‑140 (MQ=255)                       gtgAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATaagaa                                                                                                                 <  1:727401/140‑1 (MQ=255)                            aTCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAg                                                                                                            <  1:623092/140‑1 (MQ=255)                            aTCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAg                                                                                                            <  1:608584/140‑1 (MQ=255)                             tCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGc                                                                                                           >  2:310749/1‑140 (MQ=255)                             tCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGc                                                                                                           <  1:748959/140‑1 (MQ=255)                             tCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGc                                                                                                           <  1:60510/140‑1 (MQ=255)                                 tCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGa                                                                                                       <  2:358380/140‑1 (MQ=255)                                  cGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAg                                                                                                      <  2:627430/140‑1 (MQ=255)                                       cAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCat                                                                                                 <  1:310749/140‑1 (MQ=255)                                               aTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTaa                                                                                         <  2:531818/140‑1 (MQ=255)                                                  gTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTc                                                                                      >  1:527360/1‑140 (MQ=255)                                                  gTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTc                                                                                      >  1:221440/1‑140 (MQ=255)                                                     aGGCTGGCGAACTAAGCGCCTTGCAATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAg                                                                                        >  1:125623/1‑135 (MQ=255)                                                     aGGCTGGCGAACTAAGCGCCTTGCAATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAg                                                                                        <  2:125623/135‑1 (MQ=255)                                                       gCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCttttt                                                                                 <  2:221440/140‑1 (MQ=255)                                                                                    cacaATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCg                                                    >  1:400518/1‑140 (MQ=255)                                                                                       aaTGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGcc                                                 <  1:167860/140‑1 (MQ=255)                                                                                             cGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGtt                                           <  2:742479/140‑1 (MQ=255)                                                                                              ggTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGttt                                          >  2:303280/1‑140 (MQ=255)                                                                                                 ttCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCg                                                    >  2:100379/1‑127 (MQ=255)                                                                                                 ttCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCg                                                    <  1:100379/127‑1 (MQ=255)                                                                                                 ttCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTggg                                       >  1:678834/1‑140 (MQ=255)                                                                                                 ttCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTggg                                       >  1:638136/1‑140 (MQ=255)                                                                                                   cATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCa                                     <  1:303280/140‑1 (MQ=255)                                                                                                   cATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCa                                     >  1:400104/1‑140 (MQ=255)                                                                                                          cTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCattgattg                              >  2:528220/1‑140 (MQ=255)                                                                                                          cTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCattgattg                              >  1:862939/1‑140 (MQ=255)                                                                                                          cTGACGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCattgattg                              >  1:664807/1‑140 (MQ=255)                                                                                                             aCGCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCg                           >  2:594127/1‑140 (MQ=255)                                                                                                               gCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGtt                         <  1:476890/140‑1 (MQ=255)                                                                                                               gCTTTGTAGCGGTCATGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGtt                         <  1:404430/140‑1 (MQ=255)                                                                                                                             aTGCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGc           >  2:175377/1‑140 (MQ=255)                                                                                                                               gCGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCg         >  2:235418/1‑140 (MQ=255)                                                                                                                                cGGTTAATACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCgg        <  1:175377/140‑1 (MQ=255)                                                                                                                                     aaTACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGGGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTgggg   >  2:284075/1‑140 (MQ=255)                                                                                                                                     aaTACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTgggg   >  1:365183/1‑140 (MQ=255)                                                                                                                                     aaTACGCAGCAGTNGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTgggg   >  1:759898/1‑140 (MQ=255)                                                                                                                                      aTACGCAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTggggg  <  1:618021/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGG  >  NC_000913/396461‑396733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑