Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	506,843	A→G	G80G (GGT→GGC)	ybaK ←	Cys‑tRNA(Pro) and Cys‑tRNA(Cys) deacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	506,843	0	A	G	100.0%	132.3 / NA	39	G80G (GGT→GGC)	ybaK	Cys‑tRNA(Pro) and Cys‑tRNA(Cys) deacylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/18);  total (21/18)

GCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCACCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCTAAT  >  NC_000913/506712‑506975                                                                                                                                    |                                                                                                                                     gCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAgcgc                                                                                                                              >  2:822880/1‑140 (MQ=255) gCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAgcgc                                                                                                                              >  2:629772/1‑140 (MQ=255)                ggggCGTCGATAATCGTTGGCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACtttttt                                                                                                               >  1:819054/1‑140 (MQ=255)                 gggCGTCGATAATCGTTGGCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTa                                                                                                              >  2:507295/1‑140 (MQ=255)                    cGTCGATAATCGTTGGCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGa                                                                                                           <  1:629772/140‑1 (MQ=255)                                ttGGCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCt                                                                                               <  1:277274/140‑1 (MQ=255)                                ttGGCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCt                                                                                               >  2:65220/1‑140 (MQ=255)                                   gCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGCATCCCGTCGAACGCTGCGCGACCATGGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCg                                                                                            >  2:627183/1‑140 (MQ=255)                                     aGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGAc                                                                                          <  1:825038/140‑1 (MQ=255)                                                 tGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGc                                                                              <  2:819054/140‑1 (MQ=255)                                                        gTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCa                                                                            >  1:339509/1‑135 (MQ=255)                                                        gTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCa                                                                            <  2:339509/135‑1 (MQ=255)                                                        gTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCa                                                                       <  1:121168/140‑1 (MQ=255)                                                        gTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCa                                                                       >  2:678799/1‑140 (MQ=255)                                                        gTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCa                                                                       >  1:109896/1‑140 (MQ=255)                                                          gggCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAg                                                                     <  2:109896/140‑1 (MQ=255)                                                             cTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGgtg                                                                  <  1:388890/140‑1 (MQ=255)                                                                aTCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGttt                                                               <  2:745240/140‑1 (MQ=255)                                                                aTCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGttt                                                               >  1:427435/1‑140 (MQ=255)                                                                          ccAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCg                                                     >  1:547735/1‑140 (MQ=255)                                                                             ggTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTc                                                  >  2:237304/1‑140 (MQ=255)                                                                                    cGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGcca                                           >  2:587439/1‑140 (MQ=255)                                                                                     gTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGccac                                          >  1:727026/1‑140 (MQ=255)                                                                                        gAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCa                                                 >  2:435636/1‑130 (MQ=255)                                                                                        gAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCa                                                 <  1:435636/130‑1 (MQ=255)                                                                                             cTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCg                                  <  2:427435/140‑1 (MQ=255)                                                                                             cTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCg                                  <  1:627183/140‑1 (MQ=255)                                                                                               gcgcGACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGtt                                >  1:770875/1‑140 (MQ=255)                                                                                                  cgACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTg                             <  1:587439/140‑1 (MQ=255)                                                                                                  cgACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTg                             <  2:770875/140‑1 (MQ=255)                                                                                                   gACCATCGGATCGGCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGt                            <  1:65220/140‑1 (MQ=255)                                                                                                        tcggatcggCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGAcc                       >  1:760395/1‑140 (MQ=255)                                                                                                               ggCCATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCg                >  1:228474/1‑140 (MQ=255)                                                                                                                  cATCTCAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGAt             <  2:88682/140‑1 (MQ=255)                                                                                                                      tcAACTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCaa         >  1:242901/1‑140 (MQ=255)                                                                                                                          cTTTCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCt     >  2:524663/1‑140 (MQ=255)                                                                                                                             tCTTGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCTAAt  <  2:228474/140‑1 (MQ=255)                                                                                                                               ttGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATc                  <  1:474541/122‑1 (MQ=255)                                                                                                                               ttGGCGCCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATc                  >  2:474541/1‑122 (MQ=255)                                                                                                                                    |                                                                                                                                     GCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCACCCAGCGCTTTTGCTACTTTTTTAAGATCCAGTTGACCTGCGACCGGCGTAACGGCCACGGCAAGGTGTTTCATATCACCGTTCACTGCCACCAGCAGCGTTTTGTAGACCTGATCCGGATTCAAACCTAAT  >  NC_000913/506712‑506975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑