Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	513,542	C→T	R323W (CGG→TGG)	ybaT →	putative transporter YbaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	513,542	0	C	T	100.0%	168.3 / NA	50	R323W (CGG→TGG)	ybaT	putative transporter YbaT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/21);  total (29/21)

TATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGAT  >  NC_000913/513407‑513678                                                                                                                                        |                                                                                                                                         tATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCa                                                                                                                                      >  2:410801/1‑140 (MQ=255) tATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCa                                                                                                                                      >  2:357265/1‑140 (MQ=255)   tgtgATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCaga                                                                                                                                    <  1:410801/140‑1 (MQ=255)   tgtgATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCaga                                                                                                                                    <  1:593251/140‑1 (MQ=255)        tcgtcgTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTAcc                                                                                                                               >  2:746185/1‑140 (MQ=255)        tcgtcgTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTAcc                                                                                                                               >  2:167932/1‑140 (MQ=255)         cgtcgtCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTTCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCt                                                                                                                              >  2:619801/1‑140 (MQ=255)          gtcgtcATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTg                                                                                                                             >  2:293255/1‑140 (MQ=255)           tcgtcATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTgg                                                                                                                            <  2:765533/140‑1 (MQ=255)              tcATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGc                                                                                                                         <  1:293255/140‑1 (MQ=255)                  cGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGATGGCAGAGTACCTGGGGCAACa                                                                                                                     >  1:612148/1‑140 (MQ=255)                     ggcTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACatta                                                                                                                  <  1:668618/139‑1 (MQ=255)                           aCTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCg                                                                                                            <  1:619801/140‑1 (MQ=255)                              ggCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTc                                                                                                             >  1:761314/1‑136 (MQ=255)                              ggCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTc                                                                                                             <  2:761314/136‑1 (MQ=255)                               gCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCgtggt                                                                                                        >  2:242734/1‑140 (MQ=255)                                  aCGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGtt                                                                                                     <  1:746185/140‑1 (MQ=255)                                    ggCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCgt                                                                                                           >  1:680929/1‑132 (MQ=255)                                    ggCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCgt                                                                                                           <  2:680929/132‑1 (MQ=255)                                                tAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCtgatga                                                                                       >  2:149176/1‑140 (MQ=255)                                                tAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCtgatga                                                                                       >  1:154695/1‑140 (MQ=255)                                                         ccTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCAc                                                                              <  1:357265/140‑1 (MQ=255)                                                          cTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACt                                                                             <  1:242734/140‑1 (MQ=255)                                                             ttCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGaa                                                                          >  2:343359/1‑140 (MQ=255)                                                             ttCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGaa                                                                          >  2:796025/1‑140 (MQ=255)                                                                  cGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTCGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAg                                                                     <  2:603266/140‑1 (MQ=255)                                                                      tttAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTc                                                                 >  2:641248/1‑140 (MQ=255)                                                                           catcatGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCg                                                            <  1:661852/140‑1 (MQ=255)                                                                              catGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCa                                                         >  1:739176/1‑140 (MQ=255)                                                                               atGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAg                                                        >  1:744643/1‑140 (MQ=255)                                                                                tGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGc                                                       >  2:48319/1‑140 (MQ=255)                                                                                tGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGc                                                       >  1:108885/1‑140 (MQ=255)                                                                                tGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGc                                                       >  2:640832/1‑140 (MQ=255)                                                                                tGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGc                                                       >  1:481035/1‑140 (MQ=255)                                                                                 ggACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCg                                                      <  1:106362/140‑1 (MQ=255)                                                                                       caTGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCa                                                >  2:471645/1‑140 (MQ=255)                                                                                          gggCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAgcg                                             <  1:640832/140‑1 (MQ=255)                                                                                               gcgaacgcgaacTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGcc                                           <  2:277040/137‑1 (MQ=255)                                                                                               gcgaacgcgaacTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGcc                                           >  1:277040/1‑137 (MQ=255)                                                                                                  aacgcgaacTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCttt                                     >  2:639090/1‑140 (MQ=255)                                                                                                    cgcgaacTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCttttt                                   <  1:536781/140‑1 (MQ=255)                                                                                                          cTGCCGAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTg                             <  1:438910/140‑1 (MQ=255)                                                                                                               gAAGCTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTAcc                        >  2:176106/1‑140 (MQ=255)                                                                                                                   cTAATGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGc                    <  1:48319/140‑1 (MQ=255)                                                                                                                     aaTGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCgg                  <  1:796025/140‑1 (MQ=255)                                                                                                                       tGAATAAATCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGgtg                >  2:443460/1‑140 (MQ=255)                                                                                                                             aaTCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTtgtg          >  2:567015/1‑140 (MQ=255)                                                                                                                              aTCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTgtgg         >  2:453443/1‑140 (MQ=255)                                                                                                                               tCCCTGTGGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTgtggt        >  2:819945/1‑140 (MQ=255)                                                                                                                                   tgtgGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGc                    <  1:806496/124‑1 (MQ=255)                                                                                                                                   tgtgGTGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGc                    >  2:806496/1‑124 (MQ=255)                                                                                                                                    gtggtgGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGa   >  1:795967/1‑140 (MQ=255)                                                                                                                                    gtggtgGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGa   <  1:443460/140‑1 (MQ=255)                                                                                                                                     tggtggCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGATTctggtgtttat  <  1:176106/140‑6 (MQ=255)                                                                                                                                        |                                                                                                                                         TATGTGATCGTCGTCATCGGCGCTTTACTGGCGACGGCTTCAGCCATTAACGCGAACCTGTTCGCCGTGTTTAACATCATGGACAACATGGGCAGCGAACGCGAACTGCCGAAGCTAATGAATAAATCCCTGTGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGAT  >  NC_000913/513407‑513678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑