Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	20,601	G→T	intergenic (‑93/+214)	insA‑1 ← / ← rpsT	IS1 protein InsA/30S ribosomal subunit protein S20

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	20,601	0	G	T	100.0%	184.6 / NA	53	intergenic (‑93/+214)	insA‑1/rpsT	IS1 protein InsA/30S ribosomal subunit protein S20
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/26);  total (27/26)

GTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCT  >  NC_000913/20468‑20736                                                                                                                                      |                                                                                                                                        gTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGt                                                                                                                                   >  2:507805/1‑140 (MQ=255)    gCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTCTGTGTGGGAGTCTg                                                                                                                                >  1:701122/1‑140 (MQ=255)        aaCAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGgaga                                                                                                                            <  2:26242/140‑1 (MQ=255)        aaCAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGgaga                                                                                                                            <  2:167931/140‑1 (MQ=255)        aaCAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGgaga                                                                                                                            <  1:507805/140‑1 (MQ=255)        aaCAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGgaga                                                                                                                            <  1:427800/140‑1 (MQ=255)                   cAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGata                                                                                                                 >  2:405576/1‑140 (MQ=255)                      cTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTc                                                                                                              >  2:238818/1‑140 (MQ=255)                              aaCAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAg                                                                                                      >  2:414881/1‑140 (MQ=255)                                cAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGaa                                                                                                    >  1:427743/1‑140 (MQ=255)                                         cTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGc                                                                                           <  2:427743/140‑1 (MQ=255)                                            gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGa                                                                                        <  1:537108/140‑1 (MQ=255)                                            gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGa                                                                                        <  1:693414/140‑1 (MQ=255)                                                 ccTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGtt                                                                                   >  1:471606/1‑140 (MQ=255)                                                 ccTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGtt                                                                                   >  2:594320/1‑140 (MQ=255)                                                    cAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGc                                                                                           <  2:562610/129‑1 (MQ=255)                                                    cAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGc                                                                                           >  1:562610/1‑129 (MQ=255)                                                            ccATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCtt                                                                        >  2:407973/1‑140 (MQ=255)                                                               tcatACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAg                                                                     >  2:850412/1‑140 (MQ=255)                                                                   acacTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCAc                                                                 >  1:771153/1‑140 (MQ=255)                                                                      cTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGaa                                                              >  2:548482/1‑140 (MQ=255)                                                                       tAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAag                                                             >  1:664800/1‑140 (MQ=255)                                                                         aaTCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGCATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAagag                                                           >  2:810861/1‑140 (MQ=255)                                                                          aTCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGt                                                          <  2:471606/140‑1 (MQ=255)                                                                          aTCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGt                                                          <  1:195464/140‑1 (MQ=255)                                                                          aTCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGt                                                          >  2:194858/1‑140 (MQ=255)                                                                          aTCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGt                                                          >  1:34564/1‑140 (MQ=255)                                                                          aTCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGt                                                          <  1:594320/140‑1 (MQ=255)                                                                           tCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGtt                                                         <  1:414881/140‑1 (MQ=255)                                                                             aGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCa                                                       <  1:211433/140‑1 (MQ=255)                                                                               tAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGt                                                          >  1:813191/1‑135 (MQ=255)                                                                               tAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGt                                                          <  2:813191/135‑1 (MQ=255)                                                                               tAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGa                                                     <  1:748601/140‑1 (MQ=255)                                                                               tAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGa                                                     <  2:677566/140‑1 (MQ=255)                                                                                      gcagcaTCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACa                                              <  2:637522/140‑1 (MQ=255)                                                                                       cagcaTCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTAc                                               >  2:686914/1‑138 (MQ=255)                                                                                       cagcaTCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTAc                                               <  1:686914/138‑1 (MQ=255)                                                                                          caTCCCCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACgg                                          <  1:643511/140‑1 (MQ=255)                                                                                          caTCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACgg                                          <  1:810861/140‑1 (MQ=255)                                                                                            tCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCa                                        >  2:714555/1‑140 (MQ=255)                                                                                               cctacctCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGt                                     <  1:238818/140‑1 (MQ=255)                                                                                                  acctCAATGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGtctc                                  >  2:257473/1‑140 (MQ=255)                                                                                                       aaTGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATg                                      <  2:122591/131‑1 (MQ=255)                                                                                                       aaTGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATg                                      >  1:122591/1‑131 (MQ=255)                                                                                                        aTGTGTATCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGa                            >  2:130079/1‑140 (MQ=255)                                                                                                              aTCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATg                                      <  2:244734/124‑1 (MQ=255)                                                                                                              aTCACAATATCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATg                                      >  1:244734/1‑124 (MQ=255)                                                                                                                     tatCCATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAAcc               >  1:456349/1‑140 (MQ=255)                                                                                                                         cATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCa           <  1:714555/140‑1 (MQ=255)                                                                                                                         cATATTCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCa           <  1:548482/140‑1 (MQ=255)                                                                                                                             ttCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCGACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAgtgt       >  1:758247/1‑140 (MQ=255)                                                                                                                              tCTTTGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAgtgtg      <  1:130079/140‑1 (MQ=255)                                                                                                                                 ttGTGTGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTtc   >  1:767646/1‑140 (MQ=255)                                                                                                                                  tgtgtgGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAAc                >  2:614961/1‑126 (MQ=255)                                                                                                                                  tgtgtgGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAAc                <  1:614961/126‑1 (MQ=255)                                                                                                                                  tgtgtgGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCg              <  2:131787/128‑1 (MQ=255)                                                                                                                                  tgtgtgGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCg              >  1:131787/1‑128 (MQ=255)                                                                                                                                  tgtgtgGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAgtgcgctcc  >  2:396908/1‑134 (MQ=255)                                                                                                                                      |                                                                                                                                        GTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCT  >  NC_000913/20468‑20736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑