Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	679,846	A→G	G113G (GGA→GGG)	ybeU →	DUF1266 domain‑containing protein YbeU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	679,846	0	A	G	100.0%	156.0 / NA	45	G113G (GGA→GGG)	ybeU	DUF1266 domain‑containing protein YbeU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/25);  total (20/25)

ATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGA  >  NC_000913/679710‑679982                                                                                                                                         |                                                                                                                                         aTTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATc                                                                                                                                       <  1:381784/140‑1 (MQ=255)  tttAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCa                                                                                                                                      <  2:364187/140‑1 (MQ=255)  tttAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCa                                                                                                                                      <  1:619342/140‑1 (MQ=255)        tGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCt                                                                                                                                <  2:707852/140‑1 (MQ=255)                 tCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATg                                                                                                                       <  2:220969/140‑1 (MQ=255)                     aCATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCg                                                                                                                   <  1:287680/140‑1 (MQ=255)                      cATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGa                                                                                                                  >  2:219243/1‑140 (MQ=255)                       aTGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGaa                                                                                                                 <  2:492692/140‑1 (MQ=255)                       aTGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGaa                                                                                                                 <  1:404823/140‑1 (MQ=255)                        tGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAAt                                                                                                                <  1:256349/140‑1 (MQ=255)                         ggCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATg                                                                                                               >  2:720253/1‑140 (MQ=255)                          gCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATgg                                                                                                              >  2:460643/1‑140 (MQ=255)                                    tCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTc                                                                                                    <  1:219243/140‑1 (MQ=255)                                           aaGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTc                                                                                             >  1:552830/1‑140 (MQ=255)                                            agagTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCg                                                                                            <  2:711347/140‑1 (MQ=255)                                                tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCg                                                                                            <  2:838956/136‑1 (MQ=255)                                                tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCg                                                                                            >  1:838956/1‑136 (MQ=255)                                                   aaaGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCt                                                                                     <  2:23165/140‑1 (MQ=255)                                                     aGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTca                                                                                   >  1:15847/1‑140 (MQ=255)                                                             ttGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTgg                                                                           >  1:774263/1‑140 (MQ=255)                                                             ttGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTgg                                                                           <  2:39030/140‑1 (MQ=255)                                                                 tggtggCTCGGCGAGTGGGTTGATTTCTAACCAAAACGTTGCCCAAACGGCTATGCGCTGTGGGGAAGGGGGGATCAAAGACTGGGATTATGTTCGAATGGGGTTTCTGTGACGGGTCGGTGTGCTCAACAAGTGGCtta                                                                       >  2:527088/1‑138 (MQ=255)                                                                 tggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                       <  1:293883/140‑1 (MQ=255)                                                                  ggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGAc                                                                      >  2:437855/1‑140 (MQ=255)                                                                                agGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTAGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGtt                                                        >  1:379138/1‑140 (MQ=255)                                                                                    ttGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATg                                                    >  1:845314/1‑140 (MQ=255)                                                                                          tataCCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCa                                              >  1:28602/1‑140 (MQ=255)                                                                                              cccAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATcg                                          <  1:390603/140‑1 (MQ=255)                                                                                                cAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                        <  1:437855/140‑1 (MQ=255)                                                                                                 aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                       >  2:219477/1‑140 (MQ=255)                                                                                                 aaTTCGTTACCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                       >  2:598304/1‑140 (MQ=255)                                                                                                   ttCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                     >  1:779187/1‑140 (MQ=255)                                                                                                         gCCCAAACCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGt                               >  2:465918/1‑140 (MQ=255)                                                                                                              aaCCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                          <  2:15847/140‑1 (MQ=255)                                                                                                              aaCCGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                          <  2:28602/140‑1 (MQ=255)                                                                                                                ccGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                        <  1:325038/140‑1 (MQ=255)                                                                                                                ccGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                        <  1:231596/140‑1 (MQ=255)                                                                                                                ccGCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                        <  2:406578/140‑1 (MQ=255)                                                                                                                  gCTATGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCa                      <  1:598304/140‑1 (MQ=255)                                                                                                                     aTGTGCTGTGGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCa                   <  2:293956/140‑1 (MQ=255)                                                                                                                           tgtgGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                        <  2:751331/129‑1 (MQ=255)                                                                                                                           tgtgGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                        >  1:751331/1‑129 (MQ=255)                                                                                                                           tgtgGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTa             >  2:89356/1‑140 (MQ=255)                                                                                                                           tgtgGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTa             >  1:694263/1‑140 (MQ=255)                                                                                                                             tgGGGAAGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATc           >  2:396318/1‑140 (MQ=255)                                                                                                                                  aaGGGGGGATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGc      >  2:702933/1‑140 (MQ=255)                                                                                                                                      ggggATCAAAGCCTAGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGa  <  2:379138/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGA  >  NC_000913/679710‑679982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑