Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	815,002	C→T	R493C (CGT→TGT)	uvrB →	excision nuclease subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	815,002	0	C	T	100.0%	160.4 / NA	46	R493C (CGT→TGT)	uvrB	excision nuclease subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/22);  total (24/22)

GTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAG  >  NC_000913/814870‑815136                                                                                                                                     |                                                                                                                                       gTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTggg                                                                                                                                 <  1:397184/140‑1 (MQ=255) gTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTggg                                                                                                                                 <  1:3076/140‑1 (MQ=255)          accacACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGa                                                                                                                        <  1:501497/140‑1 (MQ=255)              cacTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTg                                                                                                                    <  2:669908/140‑1 (MQ=255)                   aCCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTg                                                                                                                    <  1:202360/135‑1 (MQ=255)                   aCCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTg                                                                                                                    >  2:202360/1‑135 (MQ=255)                   aCCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGt                                                                                                               >  1:87699/1‑140 (MQ=255)                   aCCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGt                                                                                                               >  2:609551/1‑140 (MQ=255)                      aaGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAgg                                                                                                            <  1:405261/140‑1 (MQ=255)                      aaGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAgg                                                                                                            <  2:469322/140‑1 (MQ=255)                        gCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGa                                                                                                          <  1:609551/140‑1 (MQ=255)                           gATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCa                                                                                                       >  2:382495/1‑140 (MQ=255)                             tGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAAc                                                                                                     >  2:789905/1‑140 (MQ=255)                                       tCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGcgcg                                                                                           <  1:705834/140‑1 (MQ=255)                                        cTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGa                                                                                          <  1:789905/140‑1 (MQ=255)                                            ccGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGt                                                                                      <  1:159428/140‑1 (MQ=255)                                                atatCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTgg                                                                                  >  2:58682/1‑140 (MQ=255)                                                 tatCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGa                                                                                 >  1:310177/1‑140 (MQ=255)                                                      cGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGc                                                                            >  1:442484/1‑140 (MQ=255)                                                                  cGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGc                                                                >  2:372574/1‑140 (MQ=255)                                                                     gcgcGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCACGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCtgg                                                             >  2:279918/1‑140 (MQ=255)                                                                        cgtgcgtTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCtggtgg                                                          <  1:382495/140‑1 (MQ=255)                                                                        cgtgcgtTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCtggtgg                                                          <  1:58682/140‑1 (MQ=255)                                                                         gtgcgtTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGc                                                         <  2:310177/140‑1 (MQ=255)                                                                          tgcgtTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCg                                                        <  1:403683/140‑1 (MQ=255)                                                                                        tCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGa                                          >  1:370757/1‑140 (MQ=255)                                                                                               tCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAaagaa                                   >  2:48416/1‑140 (MQ=255)                                                                                                cGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTg                                           <  2:86661/131‑1 (MQ=255)                                                                                                cGACACCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTg                                           >  1:86661/1‑131 (MQ=255)                                                                                                    acCGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCtt                              >  1:565781/1‑140 (MQ=255)                                                                                                      cGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTcc                            >  1:265117/1‑140 (MQ=255)                                                                                                      cGTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTcc                            >  2:393596/1‑140 (MQ=255)                                                                                                       gTCGAACGTATGGAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCt                           <  2:862158/140‑1 (MQ=255)                                                                                                                  ggAGATTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAc                >  2:797071/1‑140 (MQ=255)                                                                                                                      aTTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttc            >  2:550483/1‑140 (MQ=255)                                                                                                                      aTTATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttc            >  2:316437/1‑140 (MQ=255)                                                                                                                       ttATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcg           <  2:565781/140‑1 (MQ=255)                                                                                                                       ttATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcg           >  2:659509/1‑140 (MQ=255)                                                                                                                        tATCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcgt          >  1:643229/1‑140 (MQ=255)                                                                                                                         aTCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcgtt         >  1:690255/1‑140 (MQ=255)                                                                                                                         aTCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcgtt         >  1:559800/1‑140 (MQ=255)                                                                                                                          tCCGCGACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTg        >  2:831389/1‑140 (MQ=255)                                                                                                                            cgcgACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGAt      <  1:47788/140‑1 (MQ=255)                                                                                                                              cgACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATcc    <  1:393596/140‑1 (MQ=255)                                                                                                                              cgACTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATcc    <  1:279918/140‑1 (MQ=255)                                                                                                                                aCTTGTGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAg  <  2:442484/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GTACTGGTCACCACACTGACCAAGCGGATGGCGGAAGATCTTACCGAATATCTCGAAGAACATGGCGAGCGCGTGCGTTATCTTCACTCAGATATCGACACCGTCGAACGTATGGAGATTATCCGCGACTTGCGTCTGGGTGAGTTCGACGTGCTGGTAGGGATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCAG  >  NC_000913/814870‑815136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑