Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	863,406	C→T	E36K (GAA→AAA)	ybiY ←	putative pyruvate formate‑lyase activating enzyme YbiY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	863,406	0	C	T	100.0%	197.6 / NA	57	E36K (GAA→AAA)	ybiY	putative pyruvate formate‑lyase activating enzyme YbiY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/27);  total (30/27)

TTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCAT  >  NC_000913/863278‑863538                                                                                                                                 |                                                                                                                                     ttCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGac                                                                                                                           >  2:377222/1‑140 (MQ=255) ttCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGac                                                                                                                           >  2:481726/1‑140 (MQ=255)        tGAATAAGCAAACCATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCg                                                                                                                    >  2:115477/1‑140 (MQ=255)                   aCCATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGAGTTCTGACACCAGCGGCAGCCCAGCg                                                                                                         >  2:768692/1‑140 (MQ=255)                    ccATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGa                                                                                                        >  1:174910/1‑140 (MQ=255)                    ccATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGa                                                                                                        >  2:455655/1‑140 (MQ=255)                     cATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGaa                                                                                                       <  1:854331/140‑1 (MQ=255)                     cATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGaa                                                                                                       <  1:377222/140‑1 (MQ=255)                      aTTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGaa                                                                                                       <  1:201543/139‑1 (MQ=255)                      aTTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGaa                                                                                                       >  2:201543/1‑139 (MQ=255)                         cAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGc                                                                                                   >  1:805269/1‑140 (MQ=255)                         cAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGc                                                                                                   >  1:33736/1‑140 (MQ=255)                             gcgcgcTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCttt                                                                                               >  1:309850/1‑140 (MQ=255)                              cgcgcTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTa                                                                                              <  1:455655/140‑1 (MQ=255)                              cgcgcTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTa                                                                                              >  2:173652/1‑140 (MQ=255)                                cgcTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAg                                                                                            <  2:33736/140‑1 (MQ=255)                                 gcTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGa                                                                                           >  1:403278/1‑140 (MQ=255)                                      aTCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATAc                                                                                      >  2:104829/1‑140 (MQ=255)                                         aCTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGAc                                                                                   <  2:227729/140‑1 (MQ=255)                                         aCTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGAc                                                                                   <  1:173652/140‑1 (MQ=255)                                             ccGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAg                                                                                            >  1:382839/1‑127 (MQ=255)                                             ccGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAg                                                                                            <  2:382839/127‑1 (MQ=255)                                               ggCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCg                                                                             >  2:762098/1‑140 (MQ=255)                                                gcgcGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCgg                                                                            <  2:21193/140‑1 (MQ=255)                                                         tAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCgggg                                                                   <  2:174910/140‑1 (MQ=255)                                                          aGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGAc                                                                                   >  1:835233/1‑123 (MQ=255)                                                          aGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGAc                                                                                   <  2:835233/123‑1 (MQ=255)                                                                caGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCcatcat                                                            <  2:309850/140‑1 (MQ=255)                                                                caGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCcatcat                                                            >  1:706358/1‑140 (MQ=255)                                                                caGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCcatcat                                                            >  1:251238/1‑140 (MQ=255)                                                                   cTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATggg                                                         <  2:805269/140‑1 (MQ=255)                                                                      gCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCg                                                      >  1:86127/1‑140 (MQ=255)                                                                        aGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAg                                                    <  2:142585/140‑1 (MQ=255)                                                                             tCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAgcg                                               <  1:762098/140‑1 (MQ=255)                                                                             tCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAgcg                                               <  2:706358/140‑1 (MQ=255)                                                                                  acacAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGaa                                          <  1:115477/140‑1 (MQ=255)                                                                                       gTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTg                                     <  2:345685/140‑1 (MQ=255)                                                                                        tCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGa                                    >  2:286030/1‑140 (MQ=255)                                                                                        tCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGa                                    >  2:666555/1‑140 (MQ=255)                                                                                          gTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGaaa                                  <  1:666555/140‑1 (MQ=255)                                                                                           tGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGaaaa                                 <  2:86127/140‑1 (MQ=255)                                                                                                     agcagATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGc                       <  1:286030/140‑1 (MQ=255)                                                                                                       cagATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCct                     <  2:251238/140‑1 (MQ=255)                                                                                                       cagATCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCct                     >  1:387747/1‑140 (MQ=255)                                                                                                          aTCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGcc                      >  2:326069/1‑136 (MQ=255)                                                                                                          aTCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGcc                      <  1:326069/136‑1 (MQ=255)                                                                                                          aTCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCctctc                  >  2:38927/1‑140 (MQ=255)                                                                                                          aTCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCctctc                  >  2:262771/1‑140 (MQ=255)                                                                                                           tCCTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCctctct                 >  2:437217/1‑140 (MQ=255)                                                                                                            ccTGCGTGCGGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTa                >  1:606320/1‑140 (MQ=255)                                                                                                               gcgtgcgGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTAttt             >  2:797487/1‑140 (MQ=255)                                                                                                                 gtgcgGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCg           >  2:706981/1‑140 (MQ=255)                                                                                                                    cgGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCctctct                 <  1:810980/131‑1 (MQ=255)                                                                                                                    cgGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCctctct                 >  2:810980/1‑131 (MQ=255)                                                                                                                    cgGGCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTc        <  1:797487/140‑1 (MQ=255)                                                                                                                     gggCGCGGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCa       <  2:182907/140‑1 (MQ=255)                                                                                                                          cgGCTTTTCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCAt  <  1:104829/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     TTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCAT  >  NC_000913/863278‑863538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑