Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	868,374	A→G	R285R (CGA→CGG)	gsiA →	glutathione ABC transporter ATP binding subunit GsiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	868,374	0	A	G	100.0%	143.3 / NA	42	R285R (CGA→CGG)	gsiA	glutathione ABC transporter ATP binding subunit GsiA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/20);  total (22/20)

GGTGATGTATCAGGGCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTT  >  NC_000913/868239‑868508                                                                                                                                        |                                                                                                                                        ggTGATGTATCAGGGCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGtt                                                                                                                                     >  1:11757/1‑140 (MQ=255)  gTGATGTATCAGGGCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGttt                                                                                                                                    <  2:731204/140‑1 (MQ=255)      tGTATCAGGGCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCg                                                                                                                                >  2:766838/1‑140 (MQ=255)      tGTATCAGGGCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCg                                                                                                                                >  1:608603/1‑140 (MQ=255)          tCAGGGCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGttt                                                                                                                                    <  2:669675/132‑1 (MQ=255)          tCAGGGCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGttt                                                                                                                                    >  1:669675/1‑132 (MQ=255)             gggCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGatat                                                                                                                         >  1:275334/1‑140 (MQ=255)              ggCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGttt                                                                                                                                    >  2:460390/1‑128 (MQ=255)              ggCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGttt                                                                                                                                    <  1:460390/128‑1 (MQ=255)                  aGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCtt                                                                                                                    >  1:202549/1‑140 (MQ=255)                  aGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGAGTATCCCCGGCGTTTCCCGTTGATATCGCtt                                                                                                                    >  2:531298/1‑140 (MQ=255)                                  tACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAAc                                                                                                    >  1:155559/1‑140 (MQ=255)                                    ccGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAg                                                                                                  <  2:11757/140‑1 (MQ=255)                                    ccGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAg                                                                                                  <  1:415926/140‑1 (MQ=255)                                     cGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAgg                                                                                                 >  1:496358/1‑140 (MQ=255)                                      gTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑c                                                                                               <  1:766838/140‑1 (MQ=255)                                         gAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑cccc                                                                                            >  1:719456/1‑140 (MQ=255)                                                         cACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGaaa                                                                            <  2:496358/140‑1 (MQ=255)                                                                        acacCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGATTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGC‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGc                                                             >  2:474061/1‑140 (MQ=255)                                                                         cacCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCg                                                            <  2:202549/140‑1 (MQ=255)                                                                            ccGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                        <  2:688155/140‑1 (MQ=255)                                                                             cGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                        <  2:608603/140‑1 (MQ=255)                                                                              gTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCt                                                       >  2:649147/1‑140 (MQ=255)                                                                               tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTg                                                      >  2:411814/1‑140 (MQ=255)                                                                                 cgcTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGtt                                                    >  1:111615/1‑140 (MQ=255)                                                                                      ttAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTAc                                                >  2:139756/1‑140 (MQ=255)                                                                                       tAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGa                                              <  1:649147/140‑1 (MQ=255)                                                                                              tgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGa                                              <  1:439361/133‑1 (MQ=255)                                                                                              tgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGa                                              >  2:439361/1‑133 (MQ=255)                                                                                              tgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                       >  1:614484/1‑140 (MQ=255)                                                                                              tgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                       <  1:411814/140‑1 (MQ=255)                                                                                              tgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                       >  2:700164/1‑140 (MQ=255)                                                                                                  ccGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                       >  1:336946/1‑136 (MQ=255)                                                                                                  ccGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                       <  2:336946/136‑1 (MQ=255)                                                                                                     cAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGt                                <  2:203870/140‑1 (MQ=255)                                                                                                         ttGGTGCGATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAc                             <  1:139756/140‑1 (MQ=255)                                                                                                                       gggTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTgcg              <  1:531298/140‑1 (MQ=255)                                                                                                                         gTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCa            <  1:700164/140‑1 (MQ=255)                                                                                                                            aGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg         <  1:660593/140‑1 (MQ=255)                                                                                                                            aGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg         <  2:719456/140‑1 (MQ=255)                                                                                                                            aGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg         >  1:127765/1‑140 (MQ=255)                                                                                                                                  tCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgt   >  1:604949/1‑140 (MQ=255)                                                                                                                                   ccccGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTgcgc             <  2:542746/129‑1 (MQ=255)                                                                                                                                   ccccGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTgcgc             >  1:542746/1‑129 (MQ=255)                                                                                                                                   ccccGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgtt  <  2:155559/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GGTGATGTATCAGGGCGAGGCGGTGGAAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTT  >  NC_000913/868239‑868508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑