Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,343	A→G	intergenic (+50/+23)	macB → / ← cspD	ABC‑type tripartite efflux pump ATP binding/membrane subunit/DNA replication inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,343	0	A	G	100.0%	103.0 / NA	30	intergenic (+50/+23)	macB/cspD	ABC‑type tripartite efflux pump ATP binding/membrane subunit/DNA replication inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/16);  total (14/16)

CTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTT  >  NC_000913/922206‑922471                                                                                                                                          |                                                                                                                                 cTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTg                                                                                                                                >  2:845195/1‑140 (MQ=255) cTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTg                                                                                                                                >  1:88332/1‑140 (MQ=255)   cGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTa                                                                                                                              <  2:88332/140‑1 (MQ=255)   cGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTa                                                                                                                              <  2:804232/140‑1 (MQ=255)        gTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACaa                                                                                                                         <  1:169526/140‑1 (MQ=255)           aCCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATga                                                                                                                      >  1:578451/1‑140 (MQ=255)             cGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATgaga                                                                                                                    <  1:146760/140‑1 (MQ=255)                            cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCg                                                                                                     >  1:690247/1‑140 (MQ=255)                                                   cACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACggg                                                                              <  2:52702/140‑1 (MQ=255)                                                   cACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACggg                                                                              <  2:740233/140‑1 (MQ=255)                                                           gATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACggg                                                                              >  2:828872/1‑132 (MQ=255)                                                           gATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACggg                                                                              <  1:828872/132‑1 (MQ=255)                                                                aGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTg                                                                 <  1:659738/140‑1 (MQ=255)                                                                     aTGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTg                                                            >  1:225717/1‑140 (MQ=255)                                                                       gCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGAt                                                          >  1:276616/1‑140 (MQ=255)                                                                         tctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTg                                                        >  2:735774/1‑140 (MQ=255)                                                                               aCGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCtttt                                                  <  2:42060/140‑1 (MQ=255)                                                                                cGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTg                                                 <  2:225717/140‑1 (MQ=255)                                                                                   gagTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGcc                                              >  1:705247/1‑140 (MQ=255)                                                                                       aaTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtg                                          >  1:381307/1‑140 (MQ=255)                                                                                       aaTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtg                                          <  1:176143/140‑1 (MQ=255)                                                                                         tttttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggt                                        >  2:517368/1‑140 (MQ=255)                                                                                              gagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACa                                   >  2:436305/1‑140 (MQ=255)                                                                                                 aTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCa                                <  2:276616/140‑1 (MQ=255)                                                                                                                 aTCGGGCTGGCATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTc                >  1:82308/1‑140 (MQ=255)                                                                                                                           cATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAg      <  2:381307/140‑1 (MQ=255)                                                                                                                           cATTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAg      <  2:82308/140‑1 (MQ=255)                                                                                                                            aTTTTGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGc     <  1:625326/140‑1 (MQ=255)                                                                                                                             ttttGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCg    <  1:851886/140‑1 (MQ=255)                                                                                                                               ttGCCTTTAGGGTGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGtt  >  1:817378/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                 CTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTT  >  NC_000913/922206‑922471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑