Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	996,138	G→A	R269R (CGC→CGT)	ssuA ←	aliphatic sulfonate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	996,138	0	G	A	100.0%	98.2 / NA	29	R269R (CGC→CGT)	ssuA	aliphatic sulfonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/15);  total (14/15)

CCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGCCTCACTAAAGGTTGCCAGTACGCCCTGAATAAAA  >  NC_000913/996002‑996273                                                                                                                                         |                                                                                                                                        ccAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACga                                                                                                                                      <  1:824465/140‑1 (MQ=255) ccAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACga                                                                                                                                      <  1:390572/140‑1 (MQ=255)   aGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACga                                                                                                                                      <  2:405353/138‑1 (MQ=255)   aGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACga                                                                                                                                      >  1:405353/1‑138 (MQ=255)   aGTTGAGTGGGCTGCCAGATGCGCTGGCGAATACCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACgatg                                                                                                                                    >  1:119921/1‑140 (MQ=255)     ttGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACgatgat                                                                                                                                  <  1:803078/140‑1 (MQ=255)        aGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCta                                                                                                                               >  2:862738/1‑140 (MQ=255)            ggCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCtaagta                                                                                                                           >  1:767901/1‑140 (MQ=255)                        cgcTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCAc                                                                                                               <  2:767901/140‑1 (MQ=255)                            ggCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGt                                                                                                           <  1:14689/140‑1 (MQ=255)                                       aCTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGcc                                                                                                >  1:218951/1‑140 (MQ=255)                                                                             tGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCgct                                                          <  1:862738/140‑1 (MQ=255)                                                                                  tttGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCgctggctg                                                     >  1:421974/1‑140 (MQ=255)                                                                                    tgctgcTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCgctggctg                                                     <  1:537664/138‑1 (MQ=255)                                                                                    tgctgcTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCgctggctg                                                     >  2:537664/1‑138 (MQ=255)                                                                                         ctgTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTa                                              >  1:186402/1‑140 (MQ=255)                                                                                                   gcAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGc                                    <  1:234960/140‑1 (MQ=255)                                                                                                   gcAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGc                                    >  1:844952/1‑140 (MQ=255)                                                                                                   gcAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGc                                    >  2:18080/1‑140 (MQ=255)                                                                                                   gcAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGc                                    <  2:407452/140‑1 (MQ=255)                                                                                                   gcAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGc                                    <  2:844952/140‑1 (MQ=255)                                                                                                   gcAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGc                                    >  1:407452/1‑140 (MQ=255)                                                                                                             cGTTAACCGGTTTGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGCCTCACTAAAg                          <  2:413579/140‑1 (MQ=255)                                                                                                                       tttGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGCCTCACTAAAGGTTGCCAGTa                >  1:558744/1‑140 (MQ=255)                                                                                                                         tGATGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGCCTCACTAAAGGTTGCCAGTACg              <  1:649965/140‑1 (MQ=255)                                                                                                                           aTGGTGGTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGCCTCACTAAAGGTTGCCAGTACGcc            <  2:268552/140‑1 (MQ=255)                                                                                                                            tggtggTAGGAGGACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGCCTCACTAAAGGTTGCCAGTACGccc           <  1:514624/140‑1 (MQ=255)                                                                                                                                 gtAGGAGGACGATGATCTAAGTAAGATGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGCCTCACTAAAGGTTGCCAGTACGCCCTGAAt      >  2:51478/1‑140 (MQ=255)                                                                                                                                     gaggACGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGCCTCACTAAAGGTTGCCAGTACGCCCTGAATaaaa  >  2:375228/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CCAGTTGAGTGGGCTGCCAGATGCGCTGGCGAATATCGACTTTTTTCGGCACCAGACGATTTTCATAAAACAGATCTGCCGTTTGCTGCTGTAAGGCGGCAACCTCGGCGTTAACCGGTTTGATGGTGGTAGGAGGGCGATGATCTAAGTAAGAGGCAATCACCGGTGCCGGTAAGCCCATCGTTTTTGCCAGTAAAGCGATGCTTTGCTCGCGCTGGCTGCGGGTTAACGCATCGGCCTCACTAAAGGTTGCCAGTACGCCCTGAATAAAA  >  NC_000913/996002‑996273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑