Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	149,599	C→T	M1M (ATG→ATA) †	panB ←	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	149,599	0	C	T	100.0%	185.0 / NA	53	M1M (ATG→ATA) †	panB	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/25);  total (28/25)

AGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAGGGATAGGTTACC  >  NC_000913/149462‑149730                                                                                                                                          |                                                                                                                                    aGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATAAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTAt                                                                                                                                   >  2:765588/1‑140 (MQ=255)   cGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATaa                                                                                                                                 <  2:730897/140‑1 (MQ=255)       tCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTa                                                                                                                             <  2:295641/140‑1 (MQ=255)       tCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTa                                                                                                                             <  1:465372/140‑1 (MQ=255)                            aGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTtgtg                                                                                                        <  2:391026/140‑1 (MQ=255)                            aGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTtgtg                                                                                                        >  1:176219/1‑140 (MQ=255)                                  tcatcaGCAAAGAGTTTGGCGAAGCTATAGTCATGAGCGGTGATGGTCGCGAAACGTTTTTTTTCCCGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCt                                                                                                  >  2:768781/1‑140 (MQ=255)                                  tcatcaGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCt                                                                                                  >  2:811527/1‑140 (MQ=255)                                  tcatcaGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCt                                                                                                  >  1:78274/1‑140 (MQ=255)                                         cAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCAttt                                                                                           >  2:291332/1‑140 (MQ=255)                                           aaGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTa                                                                                         <  1:691776/140‑1 (MQ=255)                                           aaGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTa                                                                                         <  1:492372/140‑1 (MQ=255)                                           aaGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTa                                                                                         >  1:238660/1‑140 (MQ=255)                                            agagTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTAt                                                                                        <  1:660387/140‑1 (MQ=255)                                             gagTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATc                                                                                       <  2:176219/140‑1 (MQ=255)                                              agTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGCCTGGCATTTTATCa                                                                                      >  2:657064/1‑140 (MQ=255)                                                 ttGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTc                                                                                   >  1:422482/1‑140 (MQ=255)                                                  tGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTca                                                                                  >  2:681283/1‑140 (MQ=255)                                                         gCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATtggt                                                                           <  1:811527/140‑1 (MQ=255)                                                              aGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTcaca                                                                                >  2:684652/1‑130 (MQ=255)                                                              aGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTcaca                                                                                <  1:684652/130‑1 (MQ=255)                                                                    aaGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGa                                                                >  2:810351/1‑140 (MQ=255)                                                                    aaGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGa                                                                >  1:836168/1‑140 (MQ=255)                                                                    aaGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGa                                                                >  1:369782/1‑140 (MQ=255)                                                                       cGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGAttt                                                             >  2:652519/1‑140 (MQ=255)                                                                        ggTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGCCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTa                                                            <  1:657064/140‑1 (MQ=255)                                                                          tGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCa                                                                    <  1:299796/130‑1 (MQ=255)                                                                          tGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCa                                                                    >  2:299796/1‑130 (MQ=255)                                                                          tGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATc                                                          <  1:291332/140‑1 (MQ=255)                                                                                  gcgAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTagcag                                                  <  2:488378/140‑1 (MQ=255)                                                                                  gcgAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTagcag                                                  <  1:722682/140‑1 (MQ=255)                                                                                  gcgAAACG‑TTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTagcagc                                                 >  1:166801/1‑140 (MQ=255)                                                                                          ttttttttCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCa                                          <  1:725201/140‑1 (MQ=255)                                                                                          ttttttttCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCa                                          <  2:369782/140‑1 (MQ=255)                                                                                                  ccTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGa                                  <  2:422482/140‑1 (MQ=255)                                                                                                     gTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAg                               >  1:341379/1‑140 (MQ=255)                                                                                                          tACTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGtgctg                          <  2:78274/140‑1 (MQ=255)                                                                                                            cTTCTGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTgctgct                        <  2:399176/140‑1 (MQ=255)                                                                                                                tGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAg                               <  1:671927/129‑1 (MQ=255)                                                                                                                tGCAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAg                               >  2:671927/1‑129 (MQ=255)                                                                                                                  cAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTa                  <  2:836168/140‑1 (MQ=255)                                                                                                                  cAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTa                  >  2:367834/1‑140 (MQ=255)                                                                                                                  cAGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTa                  >  1:305371/1‑140 (MQ=255)                                                                                                                   aGTAAGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTAt                 <  2:14245/140‑1 (MQ=255)                                                                                                                       aGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAgg             >  1:481823/1‑140 (MQ=255)                                                                                                                       aGGAGATGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAgg             >  1:392712/1‑140 (MQ=255)                                                                                                                          agaTGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAGGGAt          >  1:126332/1‑140 (MQ=255)                                                                                                                            aTGGTGGTCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAGGGATAg        >  1:352338/1‑140 (MQ=255)                                                                                                                               gtggtCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAGGGATAGGtt     >  2:784433/1‑140 (MQ=255)                                                                                                                               gtggtCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAGGGATAGGtt     >  1:15811/1‑140 (MQ=255)                                                                                                                                  gtCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAGGGATAGGTTAcc  <  2:126332/140‑1 (MQ=255)                                                                                                                                   tCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAGGGATAGGTTAc   <  2:371809/138‑1 (MQ=255)                                                                                                                                   tCGGTTTTATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAGGGATAGGTTAc   >  1:371809/1‑138 (MQ=255)                                                                                                                                          |                                                                                                                                    AGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGACAGTGCTGCTACGTTATCAGGGATAGGTTACC  >  NC_000913/149462‑149730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑