Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,070,394	A→G	S188P (TCC→CCC)	rutE ←	putative malonic semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,070,394	0	A	G	100.0%	141.2 / NA	41	S188P (TCC→CCC)	rutE	putative malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/15);  total (26/15)

CCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCC  >  NC_000913/1070266‑1070526                                                                                                                                 |                                                                                                                                      ccGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCa                                                                                                                            >  2:48719/1‑140 (MQ=255)     cTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGgcg                                                                                                                        <  2:762324/140‑1 (MQ=255)             tccgtGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATaaa                                                                                                                >  1:247801/1‑140 (MQ=255)               cgtGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGc                                                                                                              >  1:125434/1‑140 (MQ=255)                   gtgATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTACTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTtgc                                                                                                          >  2:776366/1‑140 (MQ=255)                     gATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTtgctg                                                                                                        >  2:519324/1‑140 (MQ=255)                        aataTTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTa                                                                                                     <  1:360299/140‑1 (MQ=255)                              gACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCa                                                                                               >  1:697010/1‑140 (MQ=255)                               aCCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTgctgct                                                                                                      >  1:18235/1‑132 (MQ=255)                               aCCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTgctgct                                                                                                      <  2:18235/132‑1 (MQ=255)                               aCCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCAt                                                                                              >  2:255180/1‑140 (MQ=255)                                ccgccgCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATa                                                                                             >  2:70597/1‑140 (MQ=255)                                ccgccgCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATa                                                                                             >  2:130512/1‑140 (MQ=255)                                    cgcgCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCg                                                                                         >  2:298371/1‑140 (MQ=255)                                      cgcCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGat                                                                                       >  1:673406/1‑140 (MQ=255)                                                     tcgcatcgcGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGAtt                                                                        >  1:662294/1‑140 (MQ=255)                                                      cgcatcgcGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTg                                                                       >  1:289296/1‑140 (MQ=255)                                                        catcgcGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGct                                                                     >  2:810216/1‑140 (MQ=255)                                                         atcgcGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATcagca                                                                            >  1:51958/1‑132 (MQ=255)                                                         atcgcGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATcagca                                                                            <  2:51958/132‑1 (MQ=255)                                                             cgAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCa                                                                <  2:289296/140‑1 (MQ=255)                                                             cgAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCa                                                                <  1:292894/140‑1 (MQ=255)                                                                             cGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑aaa                                               >  2:9950/1‑140 (MQ=255)                                                                                cAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑aaaaaa                                            >  2:145897/1‑140 (MQ=255)                                                                                    gTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAATAAGGcg                                        <  1:770277/140‑1 (MQ=255)                                                                                    gTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGcg                                        <  1:298371/140‑1 (MQ=255)                                                                                        aTAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGcgtcgt                                    <  2:697010/140‑1 (MQ=255)                                                                                          aGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTcc                                  >  2:338797/1‑140 (MQ=255)                                                                                             ttGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACg                               >  2:299118/1‑140 (MQ=255)                                                                                               gCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAAGGCGTCGTCCACgt                              >  2:688343/1‑140 (MQ=255)                                                                                                         acaGCCCGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCa                   <  1:810216/140‑1 (MQ=255)                                                                                                             cccGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGc               >  1:518546/1‑140 (MQ=255)                                                                                                             cccGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGc               >  2:733702/1‑140 (MQ=255)                                                                                                             cccGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGc               >  2:649200/1‑140 (MQ=255)                                                                                                               cGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGccc             >  2:62316/1‑140 (MQ=255)                                                                                                               cGCAGGCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGccc             >  2:292051/1‑140 (MQ=255)                                                                                                                    gCTTCTTCAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACAt        <  1:130512/140‑1 (MQ=255)                                                                                                                      ttcttcAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCg      <  2:662294/140‑1 (MQ=255)                                                                                                                      ttcttcAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCg      <  2:866313/140‑1 (MQ=255)                                                                                                                        cttcAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCggg    <  1:255180/140‑1 (MQ=255)                                                                                                                          tcAAAGGGCAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGcc  <  2:278069/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      CCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGT‑AAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCC  >  NC_000913/1070266‑1070526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑