Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,220,971	G→A	pseudogene (1371/2648 nt)	ycgH →	putative transporter component, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,220,971	0	G	A	100.0%	210.9 / NA	60	pseudogene (1371/2648 nt)	ycgH	putative transporter component, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/23);  total (37/23)

ACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCGGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTC  >  NC_000913/1220841‑1221101                                                                                                                                   |                                                                                                                                   aCAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGAc                                                                                                                           >  2:786340/1‑140 (MQ=255) aCAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATAAGCTACGGAc                                                                                                                           >  2:734255/1‑140 (MQ=255)   aGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAg                                                                                                                         <  2:453751/140‑1 (MQ=255)     tattatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGAc                                                                                                                       >  2:241600/1‑140 (MQ=255)     tattatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGAc                                                                                                                       >  2:697599/1‑140 (MQ=255)      attatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACt                                                                                                                      >  2:104226/1‑140 (MQ=255)       ttatCAATCTTTCGTATGATGATGGTC‑AACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGc                                                                                                                                  >  1:702307/1‑126 (MQ=255)        tatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTa                                                                                                                    <  1:241600/140‑1 (MQ=255)             aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                               <  1:198357/140‑1 (MQ=255)             aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                               >  2:443241/1‑140 (MQ=255)               cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                               >  2:786231/1‑138 (MQ=255)               cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                               <  1:786231/138‑1 (MQ=255)               cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                             >  1:611461/1‑140 (MQ=255)               cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                             >  1:680182/1‑140 (MQ=255)               cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                             >  1:301046/1‑140 (MQ=255)                          tgatgGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACtt                                                                                                  <  2:301046/140‑1 (MQ=255)                            atgGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCg                                                                                                >  2:267787/1‑140 (MQ=255)                            atgGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATAAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCg                                                                                                <  1:734255/140‑1 (MQ=255)                               gTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGt                                                                                             >  2:382850/1‑140 (MQ=255)                                tCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                            <  2:611461/140‑1 (MQ=255)                                    aCATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTc                                                                                                 <  2:738853/131‑1 (MQ=255)                                    aCATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAAACAGCTACGGACAGACTTATTGTTGAGGGTAATACTTc                                                                                                 >  1:738853/1‑131 (MQ=255)                                      aTTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTa                                                                                      <  1:697599/140‑1 (MQ=255)                                          aCCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGt                                                                                  >  1:147959/1‑140 (MQ=255)                                                   aaaaCATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGaa                                                                         <  1:267787/140‑1 (MQ=255)                                                     aaCATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGaaaa                                                                       >  2:601094/1‑140 (MQ=255)                                                           aaCCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTg                                                                 >  1:439225/1‑140 (MQ=255)                                                                tAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTggagga                                                            >  2:573515/1‑140 (MQ=255)                                                                tAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTggagga                                                            >  1:11034/1‑140 (MQ=255)                                                                         aTTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcg                                                   >  2:235349/1‑140 (MQ=255)                                                                          ttATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcgg                                                  <  2:700504/140‑1 (MQ=255)                                                                           tATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcggc                                                 <  2:147959/140‑1 (MQ=255)                                                                               tCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACg                                             >  2:169382/1‑140 (MQ=255)                                                                               tCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACg                                             >  1:412957/1‑140 (MQ=255)                                                                               tCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACg                                             >  1:488868/1‑140 (MQ=255)                                                                                 ggTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCt                                           >  2:354354/1‑140 (MQ=255)                                                                                     ataatGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAAc                                       >  2:589955/1‑140 (MQ=255)                                                                                     ataatGGTAAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAAc                                       >  2:199140/1‑140 (MQ=255)                                                                                         tGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGa                                   <  1:439360/140‑1 (MQ=255)                                                                                         tGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGa                                   <  1:573515/140‑1 (MQ=255)                                                                                           gTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcggc                                                 <  2:262330/124‑1 (MQ=255)                                                                                           gTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcggc                                                 >  1:262330/1‑124 (MQ=255)                                                                                           gTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                 <  1:235349/140‑1 (MQ=255)                                                                                                cTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                            <  1:169382/140‑1 (MQ=255)                                                                                                cTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                            <  2:439225/140‑1 (MQ=255)                                                                                                  tAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCa                          <  2:680182/140‑1 (MQ=255)                                                                                                         cGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgt                   >  2:42263/1‑140 (MQ=255)                                                                                                          gCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                 >  1:702557/1‑125 (MQ=255)                                                                                                          gCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                 <  2:702557/125‑1 (MQ=255)                                                                                                          gCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgtg                  >  1:671604/1‑140 (MQ=255)                                                                                                          gCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgtg                  >  2:116770/1‑140 (MQ=255)                                                                                                          gCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgtg                  >  2:407809/1‑140 (MQ=255)                                                                                                           cACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGa                 <  1:42263/140‑1 (MQ=255)                                                                                                               gTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATgg             >  2:764736/1‑140 (MQ=255)                                                                                                                   tAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGAt         <  1:764736/140‑1 (MQ=255)                                                                                                                        gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtc    >  1:800805/1‑140 (MQ=255)                                                                                                                          tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc  >  1:251809/1‑140 (MQ=255)                                                                                                                          tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc  <  1:354354/140‑1 (MQ=255)                                                                                                                          tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc  >  2:492945/1‑140 (MQ=255)                                                                                                                          tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc  >  2:864998/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                   ACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCGGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTC  >  NC_000913/1220841‑1221101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑