Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,226,800	A→G	L67L (TTA→TTG)	ycgJ →	PF05666 family protein YcgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,226,800	0	A	G	100.0%	161.7 / NA	48	L67L (TTA→TTG)	ycgJ	PF05666 family protein YcgJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)

CGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAAC  >  NC_000913/1226664‑1226934                                                                                                                                         |                                                                                                                                       cGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCaa                                                                                                                                     >  1:776359/1‑140 (MQ=255) cGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCaa                                                                                                                                     <  1:864742/140‑1 (MQ=255) cGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCaa                                                                                                                                     >  1:589745/1‑140 (MQ=255)  gTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAAt                                                                                                                                    <  1:363093/140‑1 (MQ=255)   tCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATc                                                                                                                                   >  2:584932/1‑140 (MQ=255)       gAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTa                                                                                                                               <  1:471689/140‑1 (MQ=255)        aaaTGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTAc                                                                                                                              <  1:217701/140‑1 (MQ=255)         aaTGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACa                                                                                                                             <  2:199839/140‑1 (MQ=255)           tGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAg                                                                                                                           <  2:722912/140‑1 (MQ=255)             aCGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGc                                                                                                                         <  1:346092/140‑1 (MQ=255)               gCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTa                                                                                                                       <  1:223595/140‑1 (MQ=255)                 tGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTa                                                                                                                               <  1:63724/130‑1 (MQ=255)                 tGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTa                                                                                                                               >  2:63724/1‑130 (MQ=255)                                tcCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTa                                                                                                      >  2:511858/1‑140 (MQ=255)                                    gCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACAtt                                                                                                  <  1:421697/140‑1 (MQ=255)                                       tGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACAATCGc                                                                                               >  2:100427/1‑140 (MQ=255)                                         cggcgTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTa                                                                                             >  1:717943/1‑140 (MQ=255)                                             gttgttTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACgg                                                                                         <  1:472964/140‑1 (MQ=255)                                                           gTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATg                                                                           >  2:58042/1‑140 (MQ=255)                                                                  tgtgtgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGa                                                                    >  2:368060/1‑140 (MQ=255)                                                                   gtgtgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGaa                                                                   >  1:34753/1‑140 (MQ=255)                                                                      tgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGaaaaa                                                                >  2:24428/1‑140 (MQ=255)                                                                        aaCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAtt                                                              >  1:375565/1‑140 (MQ=255)                                                                        aaCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAtt                                                              <  1:24428/140‑1 (MQ=255)                                                                             aGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTc                                                         >  2:645614/1‑140 (MQ=255)                                                                                  gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACAATCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgat                                                    <  1:100427/140‑1 (MQ=255)                                                                                   cATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgatg                                                   >  2:214961/1‑140 (MQ=255)                                                                                     tttCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTgatgat                                                 >  1:698308/1‑140 (MQ=255)                                                                                     tttCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTgatgat                                                 <  1:682305/140‑1 (MQ=255)                                                                                     tttCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTgatgat                                                 <  1:584932/140‑1 (MQ=255)                                                                                     tttCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTgatgat                                                 <  2:717943/140‑1 (MQ=255)                                                                                       tCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCg                                               <  1:678193/140‑1 (MQ=255)                                                                                                ttAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTgg                                      >  1:187107/1‑140 (MQ=255)                                                                                                ttAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTgg                                      <  2:776359/140‑1 (MQ=255)                                                                                                ttAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTgg                                      >  1:112648/1‑140 (MQ=255)                                                                                                  aaCAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgtg                                    >  1:393991/1‑140 (MQ=255)                                                                                                  aaCAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgtg                                    >  2:533380/1‑140 (MQ=255)                                                                                                   aCAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgtgt                                   >  2:824296/1‑140 (MQ=255)                                                                                                     aaGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTgg                                 <  2:187107/140‑1 (MQ=255)                                                                                                          aaTATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGt                            <  1:511858/140‑1 (MQ=255)                                                                                                                  ggCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAg                    >  1:131450/1‑140 (MQ=255)                                                                                                                       aaaaGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaa               >  2:710438/1‑140 (MQ=255)                                                                                                                         aaGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaaaa             <  2:393991/140‑1 (MQ=255)                                                                                                                               cTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatcaat       <  2:589745/140‑1 (MQ=255)                                                                                                                                 gAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAatcaatca     <  1:645614/140‑1 (MQ=255)                                                                                                                                 gAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAatcaatca     <  1:479811/140‑1 (MQ=255)                                                                                                                                    aaCTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAGTCAATCAAac  <  1:107889/140‑1 (MQ=255)                                                                                                                                    aaCTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAac  <  2:34753/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAAC  >  NC_000913/1226664‑1226934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑