Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,992	A→G	R157R (CGT→CGC)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,992	0	A	G	100.0%	184.6 / NA	54	R157R (CGT→CGC)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/29);  total (25/29)

GTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCA  >  NC_000913/177856‑178127                                                                                                                                         |                                                                                                                                        gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                      >  1:144551/1‑140 (MQ=255) gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                      >  1:173729/1‑140 (MQ=255) gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                      >  1:454332/1‑140 (MQ=255) gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                      >  2:460682/1‑140 (MQ=255) gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                      >  1:38297/1‑140 (MQ=255)       ggCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTa                                                                                                                                >  1:399328/1‑140 (MQ=255)         cTAACTTGCGGCTAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                              <  2:708414/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                              <  1:686576/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                              <  2:264435/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                              <  2:38297/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                              <  2:399328/140‑1 (MQ=255)           aaCTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGtt                                                                                                                            <  1:282131/140‑1 (MQ=255)                      aGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCatat                                                                                                                 >  2:267390/1‑140 (MQ=255)                                  tGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGGTTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAAt                                                                                                     >  2:508574/1‑140 (MQ=255)                                      tttAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgcg                                                                                                 <  2:144551/140‑1 (MQ=255)                                        tAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGcgcg                                                                                               <  2:173729/140‑1 (MQ=255)                                           aGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTAc                                                                                            <  2:107260/140‑1 (MQ=255)                                              tGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGa                                                                                         >  1:406747/1‑140 (MQ=255)                                               gTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGAt                                                                                        >  1:541187/1‑140 (MQ=255)                                                ttttCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATc                                                                                       >  1:514907/1‑140 (MQ=255)                                                 tttCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATcc                                                                                      <  2:514907/140‑1 (MQ=255)                                                     tcCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCcagca                                                                                  >  2:862694/1‑140 (MQ=255)                                                                  tCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGc                                                                     <  1:460682/140‑1 (MQ=255)                                                                   cGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGc                                                                     <  1:842632/139‑1 (MQ=255)                                                                   cGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGc                                                                     >  2:842632/1‑139 (MQ=255)                                                                      gTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGt                                                                 >  1:208073/1‑140 (MQ=255)                                                                          cTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGt                                                                 <  1:658835/136‑1 (MQ=255)                                                                          cTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGt                                                                 >  2:658835/1‑136 (MQ=255)                                                                           tGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGc                                                            >  1:790468/1‑140 (MQ=255)                                                                           tGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGc                                                            >  1:484176/1‑140 (MQ=255)                                                                                   aaTCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTg                                                    <  1:349645/140‑1 (MQ=255)                                                                                        aCTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGc                                               >  1:846042/1‑140 (MQ=255)                                                                                        aCTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGc                                               >  2:477590/1‑140 (MQ=255)                                                                                         ctctTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCg                                              <  2:208073/140‑1 (MQ=255)                                                                                         ctctTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCg                                              <  2:541187/140‑1 (MQ=255)                                                                                         ctctTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCg                                              <  2:484176/140‑1 (MQ=255)                                                                                            ttttCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGAc                                           <  1:508574/140‑1 (MQ=255)                                                                                             tttCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACt                                          >  1:239611/1‑140 (MQ=255)                                                                                                   cTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAggg                                    <  2:505119/140‑1 (MQ=255)                                                                                                    tGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAgggg                                   <  1:199177/140‑1 (MQ=255)                                                                                                     ggTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAggggg                                  <  1:477590/140‑1 (MQ=255)                                                                                                     ggTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAggggg                                  <  2:454332/140‑1 (MQ=255)                                                                                                      gTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGc                                 >  1:536688/1‑140 (MQ=255)                                                                                                      gTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGc                                 <  2:406747/140‑1 (MQ=255)                                                                                                        aaaTGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCa                               >  1:731618/1‑140 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGTGGACTCCAGGGGGCCAGTTGACGt                       >  1:132263/1‑140 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGt                       >  1:336120/1‑140 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGt                       >  2:557419/1‑140 (MQ=255)                                                                                                                    aaTGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACg                   <  2:635334/140‑1 (MQ=255)                                                                                                                              tGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAAt         <  1:862694/140‑1 (MQ=255)                                                                                                                              tGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAAt         <  2:790468/140‑1 (MQ=255)                                                                                                                              tGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAAt         <  1:846178/140‑1 (MQ=255)                                                                                                                                   aaaaaCGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGtt    <  1:557419/140‑1 (MQ=255)                                                                                                                                     aaaCGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCa  <  2:731618/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCA  >  NC_000913/177856‑178127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑