Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,311,416	C→T	P110S (CCA→TCA)	tonB →	Ton complex subunit TonB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,311,416	0	C	T	100.0%	110.3 / NA	33	P110S (CCA→TCA)	tonB	Ton complex subunit TonB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/18);  total (15/18)

ACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGC  >  NC_000913/1311280‑1311550                                                                                                                                         |                                                                                                                                       aCCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCaa                                                                                                                                     >  1:331838/1‑140 (MQ=255)     gAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAAcg                                                                                                                                 <  1:821401/140‑1 (MQ=255)      aGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAAcgc                                                                                                                                <  1:321031/140‑1 (MQ=255)             ggtAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCa                                                                                                                         <  1:131765/140‑1 (MQ=255)               tAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCaaa                                                                                                                       <  1:456637/140‑1 (MQ=255)                        aaCCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTagag                                                                                                              <  1:533148/140‑1 (MQ=255)                             gAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAGGGTACAGGAGCAGTCAAAACGCGATGt                                                                                                                           >  2:708834/1‑122 (MQ=255)                             gAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAGGGTACAGGAGCAGTCAAAACGCGATGt                                                                                                                           <  1:708834/122‑1 (MQ=255)                              aaCCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTcgcg                                                                                                         <  2:759376/139‑1 (MQ=255)                              aaCCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTcgcg                                                                                                         >  1:759376/1‑139 (MQ=255)                               aCCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTc                                                                                                       <  2:331838/140‑1 (MQ=255)                                    aGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCa                                                                                                  >  1:525282/1‑140 (MQ=255)                                     gCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAATACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCAt                                                                                                 <  2:602883/140‑1 (MQ=255)                                     gCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCAt                                                                                                 <  2:347943/140‑1 (MQ=255)                                           ccccGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGt                                                                                           >  1:245712/1‑140 (MQ=255)                                                        aaaGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATAcggc                                                                              <  2:525282/140‑1 (MQ=255)                                                                    gtggtCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCt                                                                  >  2:152261/1‑140 (MQ=255)                                                                     tggtCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTg                                                                 >  1:17195/1‑140 (MQ=255)                                                                         cATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTg                                                                 >  2:199411/1‑136 (MQ=255)                                                                         cATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTg                                                                 <  1:199411/136‑1 (MQ=255)                                                                                   ccgaagccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGc                                                   >  1:326354/1‑140 (MQ=255)                                                                                   ccgaagccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGc                                                   >  2:48212/1‑140 (MQ=255)                                                                                    cgaagccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCa                                                  >  1:169768/1‑140 (MQ=255)                                                                                     gaagccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCaa                                                 <  2:326354/140‑1 (MQ=255)                                                                                        gccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACgg                                              <  1:865020/140‑1 (MQ=255)                                                                                             aaCCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCa                                         <  1:653294/140‑1 (MQ=255)                                                                                                   aGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGc                                   >  2:828426/1‑140 (MQ=255)                                                                                                         aaCCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAg                                    <  2:488654/133‑1 (MQ=255)                                                                                                         aaCCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAg                                    >  1:488654/1‑133 (MQ=255)                                                                                                         aaCCGAAGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCg                             >  2:312167/1‑140 (MQ=255)                                                                                                              aaGCCGGTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTAc                        >  1:158288/1‑140 (MQ=255)                                                                                                                    gTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAgtgt                  <  2:158288/140‑1 (MQ=255)                                                                                                                    gTGAAAAAGGTACAGGAGCAGTCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAgtgt                  <  1:312167/140‑1 (MQ=255)                                                                                                                                      cagtCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCAcgc  <  2:398872/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       ACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGC  >  NC_000913/1311280‑1311550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑