| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,364,922 | A→G | I231V (ATT→GTT) | puuB → | gamma‑glutamylputrescine oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,364,922 | 0 | A | G | 97.6% | 133.7 / ‑4.2 | 41 | I231V (ATT→GTT) | puuB | gamma‑glutamylputrescine oxidase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (15/25); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
ATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTA > NC_000913/1364793‑1365058 | aTTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCgg < 1:410132/140‑1 (MQ=255) ttGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCggg < 1:500301/140‑1 (MQ=255) gCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGGCGCAGATCCAGCACACCACGCCCGCCGTTGTGCGAACTGGCCAAGGTCAGGTGACGGCGGAGTATTTGGTTGTTGCCCGGGaa > 2:473163/1‑140 (MQ=255) gCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAAt < 1:767396/140‑1 (MQ=255) aCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTg < 1:155722/140‑1 (MQ=255) aCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTg < 2:393775/140‑1 (MQ=255) aCGCCATCCGCCTCAACGGCGGGCGCGTGAATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTg < 1:152062/140‑1 (MQ=255) gcaaTCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCCCGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTggg < 2:864528/137‑1 (MQ=255) cGCCTCAACGGCGGGCGCGTGAATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGGCAGGTGACGGCGAAGGATGTGGTTGTCG‑CCGGGGATGCGGATCTGGGCGGTaa > 2:152062/1‑140 (MQ=255) aaCGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTggg > 1:349714/1‑128 (MQ=255) aaCGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTggg < 2:349714/128‑1 (MQ=255) tATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGaa < 2:88515/140‑1 (MQ=255) tCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcagc > 1:541166/1‑138 (MQ=255) tCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcagc < 2:541166/138‑1 (MQ=255) tCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCAt > 1:98243/1‑140 (MQ=255) tCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCAt > 1:692551/1‑140 (MQ=255) gTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTg < 1:473163/140‑1 (MQ=255) gACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCg < 2:98243/140‑1 (MQ=255) gCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCa < 2:597524/140‑1 (MQ=255) gATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGc < 1:17108/140‑1 (MQ=255) gATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGc < 1:650012/140‑1 (MQ=255) ccAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAgg > 1:109627/1‑140 (MQ=255) caccacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATca > 1:61327/1‑140 (MQ=255) caccacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATca < 1:306520/140‑1 (MQ=255) ccacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcacc > 2:567638/1‑140 (MQ=255) cacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCa < 1:825720/125‑1 (MQ=255) cacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCa > 2:825720/1‑125 (MQ=255) cacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGc < 2:841985/128‑1 (MQ=255) cacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGc > 1:841985/1‑128 (MQ=255) cacGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcacca > 2:809789/1‑140 (MQ=255) acGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcaccac > 2:642418/1‑140 (MQ=255) acGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcac < 1:679131/137‑1 (MQ=255) acGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcac > 2:679131/1‑137 (MQ=255) ccGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACgg < 1:690361/140‑1 (MQ=255) ttGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTg < 1:127970/140‑1 (MQ=255) gCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCgg < 1:50807/140‑1 (MQ=255) aaCTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCaccacc < 1:519318/124‑1 (MQ=255) aaCTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCaccacc > 2:519318/1‑124 (MQ=255) aaCTGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAg > 2:72888/1‑140 (MQ=255) tGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCgg > 2:829286/1‑134 (MQ=255) tGCCAAAGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCgg < 1:829286/134‑1 (MQ=255) aaaGGTCAGGTGACGGCGAAGTATGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGc < 2:82861/140‑1 (MQ=255) aTGTGGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAac < 2:611866/140‑1 (MQ=255) gtgGTTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAacta < 2:692551/140‑1 (MQ=255) | ATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCG‑CCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTA > NC_000913/1364793‑1365058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |