Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,628,179	G→T	T221T (ACG→ACT)	ydfG →	3‑hydroxy acid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,628,179	0	G	T	100.0%	128.9 / NA	37	T221T (ACG→ACT)	ydfG	3‑hydroxy acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/19);  total (18/19)

GGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTA  >  NC_000913/1628048‑1628315                                                                                                                                    |                                                                                                                                         ggTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTctgcctgc                                                                                                                                  >  1:627810/1‑140 (MQ=255)      ggtgggtgGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACg                                                                                                                             >  1:807839/1‑140 (MQ=255)       gtgggtggTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGt                                                                                                                            >  1:259886/1‑140 (MQ=255)        tgggtggTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGtc                                                                                                                           <  2:464867/140‑1 (MQ=255)        tgggtggTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGtc                                                                                                                           <  2:318053/140‑1 (MQ=255)           gtggtACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGtcaat                                                                                                                        >  2:100281/1‑140 (MQ=255)             ggtACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGtcaa                                                                                                                         >  2:462701/1‑137 (MQ=255)             ggtACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGtcaa                                                                                                                         <  1:462701/137‑1 (MQ=255)               tACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGtcaatatca                                                                                                                    >  1:468799/1‑140 (MQ=255)                aCCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGtcaatatcaa                                                                                                                   <  1:777060/140‑1 (MQ=255)                aCCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGtcaatatcaa                                                                                                                   <  1:100281/140‑1 (MQ=255)                  cGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGtcaatatcaata                                                                                                                 >  1:56814/1‑140 (MQ=255)                         tCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGa                                                                                                          >  1:511682/1‑140 (MQ=255)                         tCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGa                                                                                                          >  1:184823/1‑140 (MQ=255)                                     tttAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGt                                                                                              <  2:468799/140‑1 (MQ=255)                                     tttAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGt                                                                                              <  2:511682/140‑1 (MQ=255)                                          aGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTAccc                                                                                         >  1:666394/1‑140 (MQ=255)                                           ggCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCa                                                                                        <  2:214922/140‑1 (MQ=255)                                           ggCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCa                                                                                        <  1:126651/140‑1 (MQ=255)                                               aTGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAatgat                                                                                                    >  2:524269/1‑124 (MQ=255)                                               aTGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAatgat                                                                                                    <  1:524269/124‑1 (MQ=255)                                                              aaaaaaCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAAt                                                                     >  1:370646/1‑140 (MQ=255)                                                                           aaaTACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGt                                                        <  2:344970/140‑1 (MQ=255)                                                                                  gTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAAttttt                                                 >  1:102323/1‑140 (MQ=255)                                                                                     gCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTtata                                              <  2:214546/140‑1 (MQ=255)                                                                                     gCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTtata                                              <  2:414947/140‑1 (MQ=255)                                                                                       aTTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATAcc                                            <  2:800910/140‑1 (MQ=255)                                                                                        ttGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATAccc                                           <  2:259886/140‑1 (MQ=255)                                                                                                   agaTGTCAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGc                                <  2:807839/140‑1 (MQ=255)                                                                                                         cAGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGtt                          >  1:860028/1‑140 (MQ=255)                                                                                                          aGCGAAGCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTa                         >  2:84045/1‑140 (MQ=255)                                                                                                                gCCGTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCtt                   >  2:289112/1‑140 (MQ=255)                                                                                                                   gTCTGGTGGGTGTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTc                >  2:455643/1‑140 (MQ=255)                                                                                                                           ggtgTCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaaaaa        <  1:269444/140‑1 (MQ=255)                                                                                                                              gtCAACTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTg     >  2:678097/1‑140 (MQ=255)                                                                                                                                 aaCTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTa  <  1:774731/140‑1 (MQ=255)                                                                                                                                 aaCTCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTACCGGGTTATTGCTTGTCACAAAAAAGTGGTa  <  1:232679/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         GGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTA  >  NC_000913/1628048‑1628315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑