Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,746,411	G→A	intergenic (‑284/‑24)	ydhQ ← / → valV	adhesin‑like autotransporter YdhQ/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,746,411	0	G	A	100.0%	121.7 / NA	36	intergenic (‑284/‑24)	ydhQ/valV	adhesin‑like autotransporter YdhQ/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/20);  total (16/20)

GATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAG  >  NC_000913/1746278‑1746540                                                                                                                                      |                                                                                                                                  gATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCg                                                                                                                             >  2:400448/1‑140 (MQ=255) gATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCg                                                                                                                             >  2:381198/1‑140 (MQ=255) gATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCg                                                                                                                             >  2:192339/1‑140 (MQ=255) gATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCg                                                                                                                             >  1:738256/1‑140 (MQ=255)  aTCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGt                                                                                                                            >  2:681674/1‑140 (MQ=255)    ccTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTg                                                                                                                          <  1:192339/140‑1 (MQ=255)    ccTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTg                                                                                                                          <  1:400448/140‑1 (MQ=255)     cTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGt                                                                                                                         <  2:380116/140‑1 (MQ=255)             aaaTCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACa                                                                                                                 >  1:165169/1‑140 (MQ=255)               aTCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAAc                                                                                                               <  1:250178/140‑1 (MQ=255)               aTCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAAc                                                                                                               <  2:65038/140‑1 (MQ=255)                  aCCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGtt                                                                                                            >  1:286800/1‑140 (MQ=255)                   cccTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTg                                                                                                           <  2:165169/140‑1 (MQ=255)                       tGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGtt                                                                                                       >  2:665145/1‑140 (MQ=255)                              caacaaAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTCGCGTTCATAGCt                                                                                                <  2:316245/140‑1 (MQ=255)                                      tataGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGt                                                                                        <  1:381198/140‑1 (MQ=255)                                      tataGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGt                                                                                        >  2:431915/1‑140 (MQ=255)                                           ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGt                                                                                        >  1:709731/1‑135 (MQ=255)                                           ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGt                                                                                        <  2:709731/135‑1 (MQ=255)                                           ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTagag                                                                                   >  1:236516/1‑140 (MQ=255)                                           ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTagag                                                                                   <  1:665145/140‑1 (MQ=255)                                           ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTagag                                                                                   <  2:333816/140‑1 (MQ=255)                                              aTTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAg                                                                                             >  2:375227/1‑127 (MQ=255)                                              aTTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAg                                                                                             <  1:375227/127‑1 (MQ=255)                                              aTTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGcac                                                                                <  2:224335/140‑1 (MQ=255)                                                ttGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGcacca                                                                              <  2:286800/140‑1 (MQ=255)                                                           ctctGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACAtgg                                                                   <  1:754790/140‑1 (MQ=255)                                                            tctGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACAtggt                                                                  <  2:569304/140‑1 (MQ=255)                                                               gCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTggg                                                               <  1:431915/140‑1 (MQ=255)                                                                       tCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTg                                                       >  2:613814/1‑140 (MQ=255)                                                                                       aaTTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGa                                       <  1:613814/140‑1 (MQ=255)                                                                                                              cTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGT‑GGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCt               <  1:357999/140‑1 (MQ=255)                                                                                                                     ctctGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTg         >  2:157586/1‑140 (MQ=255)                                                                                                                        tGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGtt      >  2:256788/1‑140 (MQ=255)                                                                                                                         gCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTa     >  1:788916/1‑140 (MQ=255)                                                                                                                            gCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTagag  <  2:530106/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  GATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAG  >  NC_000913/1746278‑1746540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑