Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,855,947	T→C	T9A (ACG→GCG)	ydjG ←	NADH‑dependent methylglyoxal reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,855,947	0	T	C	100.0%	128.5 / NA	37	T9A (ACG→GCG)	ydjG	NADH‑dependent methylglyoxal reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/19);  total (18/19)

GATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATAGCCGCCGTTGCA  >  NC_000913/1855818‑1856077                                                                                                                                  |                                                                                                                                   gATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTaaa                                                                                                                          <  2:348956/140‑1 (MQ=255)  aTTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAg                                                                                                                         >  2:680638/1‑140 (MQ=255)  aTTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAg                                                                                                                         >  1:618158/1‑140 (MQ=255)    tAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGt                                                                                                                       <  2:304360/140‑1 (MQ=255)            caACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCttttt                                                                                                               >  2:694463/1‑140 (MQ=255)                gATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTaa                                                                                                                           <  2:174738/124‑1 (MQ=255)                gATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTaa                                                                                                                           >  1:174738/1‑124 (MQ=255)                  tGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTg                                                                                                         >  1:822959/1‑140 (MQ=255)                     gCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCt                                                                                                      <  1:257543/140‑1 (MQ=255)                            gAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTAt                                                                                               <  1:642000/140‑1 (MQ=255)                               tCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCt                                                                                            <  2:822959/140‑1 (MQ=255)                                             atTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAg                                                                              >  1:683209/1‑140 (MQ=255)                                               ttGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCa                                                                            <  2:618158/140‑1 (MQ=255)                                                  ccGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAAtt                                                                         >  2:680700/1‑140 (MQ=255)                                                   cGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTAttct                                                                                   <  1:135851/129‑1 (MQ=255)                                                   cGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTAttct                                                                                   >  2:135851/1‑129 (MQ=255)                                                    gATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTcc                                                                                <  2:431776/131‑1 (MQ=255)                                                    gATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTcc                                                                                >  1:431776/1‑131 (MQ=255)                                                       cGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCg                                                                    >  2:321860/1‑140 (MQ=255)                                                          gaTCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGAcc                                                                 <  2:556114/140‑1 (MQ=255)                                                           aTCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCa                                                                <  2:814343/140‑1 (MQ=255)                                                                       tGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGtttt                                                    >  2:138551/1‑140 (MQ=255)                                                                            gACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg                                               <  2:833494/140‑1 (MQ=255)                                                                            gACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg                                               >  1:833494/1‑140 (MQ=255)                                                                            gACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg                                               >  2:725123/1‑140 (MQ=255)                                                                            gACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg                                               >  1:110339/1‑140 (MQ=255)                                                                             aCCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGc                                              <  2:683209/140‑1 (MQ=255)                                                                              ccgccgCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGcc                                             >  2:287745/1‑140 (MQ=255)                                                                                           cccATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCg                                <  1:694463/140‑1 (MQ=255)                                                                                           cccATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCg                                <  1:360275/140‑1 (MQ=255)                                                                                               tGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGc                            >  2:117695/1‑140 (MQ=255)                                                                                                gTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCt                           <  2:156116/140‑1 (MQ=255)                                                                                                 tCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCtt                          <  1:205701/140‑1 (MQ=255)                                                                                                             ttCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATa              >  2:87281/1‑140 (MQ=255)                                                                                                             ttCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATa              >  2:42374/1‑140 (MQ=255)                                                                                                                         tAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATAGCCGCCGTTGCa  <  1:87281/140‑1 (MQ=255)                                                                                                                         tAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATAGCCGCCGTTGCa  <  1:117695/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   GATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATAGCCGCCGTTGCA  >  NC_000913/1855818‑1856077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑