Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,912,991	T→C	Y609C (TAC→TGC)	prc ←	tail‑specific protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,912,991	0	T	C	100.0%	147.1 / NA	43	Y609C (TAC→TGC)	prc	tail‑specific protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/26);  total (17/26)

TCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTA  >  NC_000913/1912859‑1913126                                                                                                                                     |                                                                                                                                        tCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCag                                                                                                                                  <  2:630221/140‑1 (MQ=255)  ccGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCaga                                                                                                                                 >  1:23649/1‑140 (MQ=255)   cGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCagag                                                                                                                                <  2:309468/140‑1 (MQ=255)    ggCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAgaga                                                                                                                               <  1:731099/140‑1 (MQ=255)    ggCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAgaga                                                                                                                               >  2:442467/1‑140 (MQ=255)            gTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGatat                                                                                                                       <  2:473764/140‑1 (MQ=255)            gTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGatat                                                                                                                       <  2:528849/140‑1 (MQ=255)                 cTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTgcgc                                                                                                                  <  2:291534/140‑1 (MQ=255)                 cTTTCGGTAGATCATCCAGTTTCTTCAACTACGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTgcgc                                                                                                                  <  2:531059/140‑1 (MQ=255)                           atcatcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCa                                                                                                        >  1:602567/1‑140 (MQ=255)                           atcatcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCa                                                                                                        >  1:73655/1‑140 (MQ=255)                               tcCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGc                                                                                                    >  1:692595/1‑140 (MQ=255)                                    tttCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGa                                                                                               >  1:382730/1‑140 (MQ=255)                                     ttcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCtt                                                                                                                >  2:652604/1‑122 (MQ=255)                                     ttcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCtt                                                                                                                <  1:652604/122‑1 (MQ=255)                                      tcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAg                                                                                             >  1:553199/1‑140 (MQ=255)                                      tcttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAg                                                                                             >  1:693951/1‑140 (MQ=255)                                       cttcAACTCCGGTTTTCCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAgc                                                                                            <  2:571380/140‑1 (MQ=255)                                       cttcAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAgc                                                                                            <  2:865533/140‑1 (MQ=255)                                          cAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAgcgcg                                                                                         >  1:723075/1‑140 (MQ=255)                                            aCTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGcgcgcg                                                                                       <  2:382730/140‑1 (MQ=255)                                             cTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGa                                                                                      <  2:692595/140‑1 (MQ=255)                                              tCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGat                                                                                     <  1:21973/140‑1 (MQ=255)                                              tCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGat                                                                                     <  1:442467/140‑1 (MQ=255)                                                  gTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATcc                                                                                 >  1:361599/1‑140 (MQ=255)                                                      aCCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCa                                                                             <  2:632265/140‑1 (MQ=255)                                                        cTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCatg                                                                           <  2:361599/140‑1 (MQ=255)                                                        cTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCatg                                                                           <  1:718823/140‑1 (MQ=255)                                                         ttCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCatga                                                                          <  2:449675/140‑1 (MQ=255)                                                          tCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGcgcgcg                                                                                       <  1:589903/126‑1 (MQ=255)                                                          tCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGcgcgcg                                                                                       >  2:589903/1‑126 (MQ=255)                                                           cgcgTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAgcgcg                                                                                         >  2:426764/1‑123 (MQ=255)                                                           cgcgTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAgcgcg                                                                                         <  1:426764/123‑1 (MQ=255)                                                             cgTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGtt                                                                      >  2:472865/1‑140 (MQ=255)                                                                     gCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACt                                                              <  2:553199/140‑1 (MQ=255)                                                                                    cgcCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGa                                               <  1:17306/140‑1 (MQ=255)                                                                                      ccAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATa                                             <  1:521282/140‑1 (MQ=255)                                                                                      ccAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATa                                             <  2:23649/140‑1 (MQ=255)                                                                                         gACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATAcgc                                          <  2:693951/140‑1 (MQ=255)                                                                                               tCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTa                                    >  1:525329/1‑140 (MQ=255)                                                                                               tCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTa                                    >  1:592663/1‑140 (MQ=255)                                                                                                           cATTATTCTCTTTCTCACGCACAGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTcagc                        >  2:145711/1‑140 (MQ=255)                                                                                                                                 aGCGCAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTa  <  1:472865/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTA  >  NC_000913/1912859‑1913126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑