Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,921,048	G→A	D276N (GAT→AAT)	rsmF →	16S rRNA m(5)C1407 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,921,048	0	G	A	100.0%	98.8 / NA	29	D276N (GAT→AAT)	rsmF	16S rRNA m(5)C1407 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/12);  total (17/12)

TTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTACCCGCCCCCA  >  NC_000913/1920928‑1921183                                                                                                                         |                                                                                                                                        ttACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAAtc                                                                                                                                      <  1:105736/124‑1 (MQ=255) ttACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAAtc                                                                                                                                      >  2:105736/1‑124 (MQ=255)     gTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGcaaacaaa                                                                                                                  >  2:811859/1‑140 (MQ=255)       ccTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAgc                                                                                                                >  1:277779/1‑140 (MQ=255)       ccTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAgc                                                                                                                >  2:730416/1‑140 (MQ=255)        cTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAgcg                                                                                                               <  2:277779/140‑1 (MQ=255)        cTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAgcg                                                                                                               <  1:862419/140‑1 (MQ=255)           gtggtACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTg                                                                                                            <  2:660106/140‑1 (MQ=255)                 cGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCgaa                                                                                                      >  1:740426/1‑140 (MQ=255)                   cTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCgaaga                                                                                                    >  2:64955/1‑140 (MQ=255)                                     aCCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGtttt                                                                                  >  1:27404/1‑140 (MQ=255)                                                 gaagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCcac                                                                             >  1:529393/1‑133 (MQ=255)                                                 gaagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCcac                                                                             <  2:529393/133‑1 (MQ=255)                                                 gaagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTa                                                                      >  1:318830/1‑140 (MQ=255)                                                               cGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGct                                                        <  1:64955/140‑1 (MQ=255)                                                                 tttGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGa                                                                   <  1:635193/127‑1 (MQ=255)                                                                 tttGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGa                                                                   >  2:635193/1‑127 (MQ=255)                                                                 tttGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGcttc                                                      >  2:596941/1‑140 (MQ=255)                                                                   tGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGcttctt                                                    <  2:740426/140‑1 (MQ=255)                                                                                   agaCTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGt                                    <  2:134835/140‑1 (MQ=255)                                                                                     aCTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTaa                                  >  1:96998/1‑140 (MQ=255)                                                                                     aCTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTaa                                  >  2:21653/1‑140 (MQ=255)                                                                                             cGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAgg                          <  1:47708/140‑1 (MQ=255)                                                                                               aCGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAAcagacgag                        <  2:27404/140‑8 (MQ=255)                                                                                               aCGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCg                        >  2:711945/1‑140 (MQ=255)                                                                                                  cAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGAtt                     <  1:596941/140‑1 (MQ=255)                                                                                                             ttACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTAcc          >  2:440135/1‑140 (MQ=255)                                                                                                               aCCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTACCCg        >  2:559452/1‑140 (MQ=255)                                                                                                                     tGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTACCCGCCCCCa  >  1:528598/1‑140 (MQ=255)                                                                                                                         |                                                                                                                                        TTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTACCCGCCCCCA  >  NC_000913/1920928‑1921183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑