Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	208,270	A→G	I1049V (ATC→GTC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	208,270	0	A	G	100.0%	83.5 / NA	26	I1049V (ATC→GTC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

CGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTATCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGA  >  NC_000913/208133‑208394                                                                                                                                          |                                                                                                                             cgcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTc                                                                                                                            >  1:375230/1‑140 (MQ=255)     ggTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCa                                                                                                                        <  2:638457/140‑1 (MQ=255)               aCCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGt                                                                                                              <  1:329882/140‑1 (MQ=255)                 cAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCT‑GAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAg                                                                                                           <  2:27406/140‑1 (MQ=255)                              cGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACtt                                                                                               <  2:576229/140‑1 (MQ=255)                                    ggTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCgg                                                                                         <  2:684511/140‑1 (MQ=255)                                    ggTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCgg                                                                                         <  1:743026/140‑1 (MQ=255)                                          tGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCt                                                                                   <  2:503335/140‑1 (MQ=255)                                               gCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTaaaa                                                                              >  1:547357/1‑140 (MQ=255)                                                cTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAAt                                                                             >  2:744321/1‑140 (MQ=255)                                                   gATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGAc                                                                          <  2:622099/140‑1 (MQ=255)                                                             ccGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGaa                                                                >  2:391408/1‑140 (MQ=255)                                                                   ggcTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATg                                                          <  1:391408/140‑1 (MQ=255)                                                                                   gttTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGa                                                            <  1:585297/122‑1 (MQ=255)                                                                                   gttTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGa                                                            >  2:585297/1‑122 (MQ=255)                                                                                             gCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAata                                >  1:411935/1‑140 (MQ=255)                                                                                              cccTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCggg                                       >  2:413990/1‑132 (MQ=255)                                                                                              cccTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCggg                                       <  1:413990/132‑1 (MQ=255)                                                                                              cccTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAatat                               >  2:814230/1‑140 (MQ=255)                                                                                                             aGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCt                >  1:242766/1‑140 (MQ=255)                                                                                                             aGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCt                >  1:342068/1‑140 (MQ=255)                                                                                                               cAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTAt              >  2:626122/1‑140 (MQ=255)                                                                                                                 aTTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTAtct            <  2:271698/140‑1 (MQ=255)                                                                                                                 aTTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTAtct            <  2:547357/140‑1 (MQ=255)                                                                                                                     cTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTGTGATGACTTCAGCGGTGGACTTAAAATGACCGCTCGCGAAGTGATGGATATTGATGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCt        >  2:772904/1‑140 (MQ=255)                                                                                                                           aaaGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGa  <  2:134213/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                             CGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTATCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGA  >  NC_000913/208133‑208394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑