BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,201,201	T→C	V308A (GTC→GCC)	yehI →	DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,201,201	0	T	C	100.0%	121.3 / NA	35	V308A (GTC→GCC)	yehI	DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/19); total (16/19)

GAAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGC  >  NC_000913/2201068‑2201337

gAAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCg                                                                                                                                    <  2:190083/140‑1 (MQ=255)

  aaaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCa                                                                                                                                  <  2:622092/140‑1 (MQ=255)

  aaaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCa                                                                                                                                  <  1:385184/140‑1 (MQ=255)

        cTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCAc                                                                                                                                 <  2:275365/135‑1 (MQ=255)

        cTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCAc                                                                                                                                 >  1:275365/1‑135 (MQ=255)

            ggCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACt                                                                                                                        <  2:493019/140‑1 (MQ=255)

                                     tGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGc                                                                                               <  1:581854/140‑1 (MQ=255)

                                          gAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCa                                                                                          <  2:511015/140‑1 (MQ=255)

                                            aCAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAAc                                                                                        >  2:615205/1‑140 (MQ=255)

                                               aGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtg                                                                                     <  1:615205/140‑1 (MQ=255)

                                                      gCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCC‑CACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGc                                                                                               <  1:118518/122‑1 (MQ=255)

                                                      gCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCC‑CACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGc                                                                                               >  2:118518/1‑122 (MQ=255)

                                                              tCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATa                                                                      >  1:468583/1‑140 (MQ=255)

                                                                cgcgCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATata                                                                    <  2:600541/140‑3 (MQ=255)

                                                                                 gCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGAc                                                   >  2:650270/1‑140 (MQ=255)

                                                                                  cccATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACg                                                  <  2:468583/140‑1 (MQ=255)

                                                                                      tAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcg                                              >  1:542464/1‑140 (MQ=255)

                                                                                      tAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcg                                              >  2:52378/1‑140 (MQ=255)

                                                                                      tAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcg                                              >  2:716785/1‑140 (MQ=255)

                                                                                      tAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcg                                              >  1:169110/1‑140 (MQ=255)

                                                                                        aagaagATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcgcc                                            >  2:9717/1‑140 (MQ=255)

                                                                                        aagaagATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcgcc                                            >  2:236711/1‑140 (MQ=255)

                                                                                           aagaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCa                                         >  1:750478/1‑140 (MQ=255)

                                                                                           aagaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCa                                         <  1:716785/140‑1 (MQ=255)

                                                                                                  cGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAg                                  <  1:650270/140‑1 (MQ=255)

                                                                                                       gTCTGCTGGTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActg                             <  1:227447/140‑1 (MQ=255)

                                                                                                              ggTACAAATTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGt                      <  1:236711/140‑1 (MQ=255)

                                                                                                                    aaTTAACCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAg                >  2:729828/1‑140 (MQ=255)

                                                                                                                         aCCATCCGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAacca           >  1:104857/1‑140 (MQ=255)

                                                                                                                              ccGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgc      >  2:258384/1‑140 (MQ=255)

                                                                                                                              ccGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgc      <  1:214572/140‑1 (MQ=255)

                                                                                                                               cGCAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcc     >  1:578358/1‑140 (MQ=255)

                                                                                                                                 cAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccg   <  2:175287/140‑1 (MQ=255)

                                                                                                                                 cAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccg   <  2:542464/140‑1 (MQ=255)

                                                                                                                                 cAAGCCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccg   <  1:258384/140‑1 (MQ=255)

                                                                                                                                  aaGCCATCCGCCCCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCGCCATGCGACGCTCGCCGCCCTGGCAGAACTGCTGGTGTTAACAGACCCACCAgcccgc  <  2:233584/140‑1 (MQ=255)

GAAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGC  >  NC_000913/2201068‑2201337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`