Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,892	A→G	T65A (ACA→GCA)	yeiI →	putative sugar kinase YeiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,892	0	A	G	100.0%	110.0 / NA	32	T65A (ACA→GCA)	yeiI	putative sugar kinase YeiI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/19);  total (13/19)

GCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGT  >  NC_000913/2251756‑2252028                                                                                                                                         |                                                                                                                                         gcagAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGcaa                                                                                                                                       <  2:653028/140‑1 (MQ=255)  cagAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGcaat                                                                                                                                      <  1:77462/140‑1 (MQ=255)  cagAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGcaat                                                                                                                                      <  2:60204/140‑1 (MQ=255)               cGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATAtt                                                                                                                         >  1:1365/1‑140 (MQ=255)                 gACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTcg                                                                                                                       <  2:160879/140‑1 (MQ=255)                 gACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTcg                                                                                                                       <  2:35966/140‑1 (MQ=255)                  aCATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTcgc                                                                                                                      <  2:572521/140‑1 (MQ=255)                             aTCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGa                                                                                                           <  1:571425/140‑1 (MQ=255)                                 gCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATc                                                                                                       >  2:333821/1‑140 (MQ=255)                                  ccGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATcc                                                                                                      >  2:465140/1‑140 (MQ=255)                                                      atatTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTtct                                                                                  <  1:178763/140‑1 (MQ=255)                                                           atGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATcc                                                                             <  1:333821/140‑1 (MQ=255)                                                           atGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATcc                                                                             <  1:303400/140‑1 (MQ=255)                                                                 ttAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTtct                                                                                  >  1:804286/1‑129 (MQ=255)                                                                 ttAATGCGCAAAGGCCGGATTAAAGGCAAAGGCTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTtct                                                                                  <  2:804286/129‑1 (MQ=255)                                                                  tAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACa                                                                      >  1:634901/1‑140 (MQ=255)                                                                  tAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACa                                                                      <  1:665090/140‑1 (MQ=255)                                                                       cgcAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCa                                                                 <  2:853388/140‑1 (MQ=255)                                                                                      aaaGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATAAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAgcgggcgg                                                  >  1:58653/1‑140 (MQ=255)                                                                                        aGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGgcggcg                                                <  2:419648/140‑1 (MQ=255)                                                                                          gCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTg                                              <  2:634901/140‑1 (MQ=255)                                                                                               ggTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACg                                         <  2:413317/140‑1 (MQ=255)                                                                                                    cATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACa                                    >  2:816761/1‑140 (MQ=255)                                                                                                     aTTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACAt                                   <  1:816761/140‑1 (MQ=255)                                                                                                      ttCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATc                                  <  2:330998/140‑1 (MQ=255)                                                                                                      ttCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATc                                  <  2:529719/140‑1 (MQ=255)                                                                                                          cACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGccc                              >  1:548991/1‑140 (MQ=255)                                                                                                          cACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGccc                              >  1:383223/1‑140 (MQ=255)                                                                                                          cACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACAGCGccc                              >  2:766648/1‑140 (MQ=255)                                                                                                                            cGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAg            >  2:424195/1‑140 (MQ=255)                                                                                                                             gTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAgg           >  1:288542/1‑140 (MQ=255)                                                                                                                                    tggGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGAc    >  1:197090/1‑140 (MQ=255)                                                                                                                                      ggggCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTg      <  2:817666/136‑1 (MQ=255)                                                                                                                                      ggggCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGt  >  2:563831/1‑140 (MQ=255)                                                                                                                                      ggggCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGt  >  2:150488/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         GCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGT  >  NC_000913/2251756‑2252028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑