Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,276,206	A→G	T312A (ACC→GCC)	yejE →	putative oligopeptide ABC transporter membrane subunit YejE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,276,206	0	A	G	100.0%	167.1 / NA	48	T312A (ACC→GCC)	yejE	putative oligopeptide ABC transporter membrane subunit YejE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/27);  total (21/27)

CGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTT  >  NC_000913/2276069‑2276331                                                                                                                                          |                                                                                                                              cGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGcc                                                                                                                             >  2:367890/1‑140 (MQ=255)                 ttCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTgg                                                                                                             >  2:516563/1‑140 (MQ=255)                 ttCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTgg                                                                                                             >  1:77185/1‑140 (MQ=255)                   cGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCg                                                                                                           <  2:830542/140‑1 (MQ=255)                   cGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCg                                                                                                           <  2:687531/140‑1 (MQ=255)                          caccCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGAtattat                                                                                                    <  1:516563/140‑1 (MQ=255)                           accCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCg                                                                                                           <  1:867683/132‑1 (MQ=255)                           accCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCg                                                                                                           >  2:867683/1‑132 (MQ=255)                             ccTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGt                                                                                                 >  1:440960/1‑140 (MQ=255)                             ccTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGt                                                                                                 <  1:649923/140‑1 (MQ=255)                                gACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCtt                                                                                              >  1:570168/1‑140 (MQ=255)                                 aCCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTgg                                                                                                             >  2:594432/1‑124 (MQ=255)                                 aCCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTgg                                                                                                             <  1:594432/124‑1 (MQ=255)                                  ccTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            <  2:440960/140‑1 (MQ=255)                                  ccTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            <  2:570168/140‑1 (MQ=255)                                    tcgctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCt                                                                                          >  1:115058/1‑140 (MQ=255)                                      gctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGa                                                                                        >  1:698027/1‑140 (MQ=255)                                       ctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGAtatt                                                                                                      <  2:760179/125‑1 (MQ=255)                                       ctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGAtatt                                                                                                      >  1:760179/1‑125 (MQ=255)                                       ctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTg                                                                                         <  1:312677/138‑1 (MQ=255)                                       ctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTg                                                                                         >  2:312677/1‑138 (MQ=255)                                           aTTTCCTCGGCTTCGGTCTGCCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCttt                                                                                   <  1:202867/140‑1 (MQ=255)                                           aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            <  2:429025/131‑1 (MQ=255)                                           aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            <  2:394042/131‑1 (MQ=255)                                           aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            >  1:429025/1‑131 (MQ=255)                                           aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            >  1:394042/1‑131 (MQ=255)                                           aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCttt                                                                                   <  1:856570/140‑1 (MQ=255)                                            tttCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTa                                                                                  <  2:146124/140‑1 (MQ=255)                                             ttCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTAt                                                                                 <  1:78484/140‑1 (MQ=255)                                                cTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTgg                                                                              >  1:51752/1‑140 (MQ=255)                                                        cGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCg                                                                      >  1:146986/1‑140 (MQ=255)                                                                         cACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGAt                                                     <  1:367890/140‑1 (MQ=255)                                                                                 cTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCtaataa                                             <  2:813254/140‑1 (MQ=255)                                                                                   cGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           <  1:253731/140‑1 (MQ=255)                                                                                   cGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           <  2:277726/140‑1 (MQ=255)                                                                                   cGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           <  2:77185/140‑1 (MQ=255)                                                                                            gctgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           >  1:796264/1‑131 (MQ=255)                                                                                            gctgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           <  2:796264/131‑1 (MQ=255)                                                                                              tgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCAt                                >  2:539090/1‑140 (MQ=255)                                                                                              tgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCAt                                <  1:539090/140‑1 (MQ=255)                                                                                                ttACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGa                              <  2:51752/140‑1 (MQ=255)                                                                                                    aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                          <  2:424967/140‑1 (MQ=255)                                                                                                     aGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCaa                         >  2:122193/1‑140 (MQ=255)                                                                                                     aGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCaa                         >  1:682408/1‑140 (MQ=255)                                                                                                       ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCCCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAc                       >  2:804149/1‑140 (MQ=255)                                                                                                        gAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAct                      <  1:333892/140‑1 (MQ=255)                                                                                                                      aGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGa        <  2:698027/140‑1 (MQ=255)                                                                                                                            cGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAAttt  >  1:164185/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                              CGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTT  >  NC_000913/2276069‑2276331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑