Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	215,181	C→T	R117C (CGC→TGC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	215,181	0	C	T	100.0%	136.3 / NA	42	R117C (CGC→TGC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/19);  total (23/19)

ACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTA  >  NC_000913/215054‑215317                                                                                                                                |                                                                                                                                         acaGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAgg                                                                                                                                     >  1:137942/1‑133 (MQ=255) acaGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAgg                                                                                                                                     <  2:137942/133‑1 (MQ=255)             cAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACaaa                                                                                                                  >  1:357923/1‑140 (MQ=255)                 cAGGAACTGAACCGCGAAGCAGCTCTGGCCAGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATc                                                                                                              <  2:563757/140‑1 (MQ=255)                  aGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCa                                                                                                             >  1:420136/1‑140 (MQ=255)                          aaCCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGaa                                                                                                     >  1:199213/1‑140 (MQ=255)                          aaCCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGaa                                                                                                     >  2:832223/1‑140 (MQ=255)                               cgAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGa                                                                                                >  1:534644/1‑140 (MQ=255)                                               cGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTgcg                                                                                <  2:420136/140‑1 (MQ=255)                                                 gCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCa                                                                              >  2:228501/1‑140 (MQ=255)                                                 gCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCa                                                                              >  2:507187/1‑140 (MQ=255)                                                      tggCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGt                                                                         >  1:216379/1‑140 (MQ=255)                                                            tGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGaa                                                                   <  2:384550/140‑1 (MQ=255)                                                                  aaGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATaaaaa                                                             >  1:842839/1‑140 (MQ=255)                                                                  aaGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATaaaaa                                                             >  1:269307/1‑140 (MQ=255)                                                                   aGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaag                                                            >  2:787126/1‑140 (MQ=255)                                                                   aGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaag                                                            <  2:199213/140‑1 (MQ=255)                                                                   aGAATTAACAACAGAAAAAAAAGCCCGACCACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCCCAAAAATCAAGCCTGAAGACGATGCGCGGCGAAGTGGGCAGCGGCCGGGAATAAAaag                                                            <  2:194509/140‑1 (MQ=255)                                                                     aaTTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaagaa                                                          <  1:680849/140‑1 (MQ=255)                                                                         aacaacAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCAGTCGGGAATAAAAAGAAGGaa                                                      >  2:796300/1‑140 (MQ=255)                                                                              caGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAatggat                                                 >  1:461487/1‑140 (MQ=255)                                                                                 aaaaaaaaGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGTGTAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGt                                              >  1:659999/1‑140 (MQ=255)                                                                                                gacCCACGCGGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGt                               <  2:842839/140‑1 (MQ=255)                                                                                                     aCGCGGCCCACCCGTGCATCTAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAg                          >  2:446172/1‑140 (MQ=255)                                                                                                        cgGCCCACCCGTGCATCGAAAGAGTGTAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGa                       <  2:659999/140‑1 (MQ=255)                                                                                                        cgGCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGa                       <  1:305472/140‑1 (MQ=255)                                                                                                          gCCCACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATg                     >  1:422744/1‑140 (MQ=255)                                                                                                            ccACCCGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGc                   <  2:357923/140‑1 (MQ=255)                                                                                                               cccGTGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGt                >  2:651003/1‑140 (MQ=255)                                                                                                                   tGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATc            <  2:422744/140‑1 (MQ=255)                                                                                                                   tGCATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATc            <  1:651003/140‑1 (MQ=255)                                                                                                                     cATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          >  2:549793/1‑140 (MQ=255)                                                                                                                     cATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          <  1:228501/140‑1 (MQ=255)                                                                                                                     cATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          <  2:761349/140‑1 (MQ=255)                                                                                                                     cATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          <  1:549793/140‑1 (MQ=255)                                                                                                                     cATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          >  1:518991/1‑140 (MQ=255)                                                                                                                     cATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          <  1:507187/140‑1 (MQ=255)                                                                                                                     cATCGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          >  1:42771/1‑140 (MQ=255)                                                                                                                        cGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          >  1:823816/1‑137 (MQ=255)                                                                                                                        cGAAAGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          <  2:823816/137‑1 (MQ=255)                                                                                                                          aaaGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          >  2:647000/1‑135 (MQ=255)                                                                                                                          aaaGAGTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAg          <  1:647000/135‑1 (MQ=255)                                                                                                                             gagTGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTa  <  2:269307/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                         ACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTA  >  NC_000913/215054‑215317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑