Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,373,305	T→C	E78E (GAA→GAG)	pmrD ←	signal transduction protein PmrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,373,305	0	T	C	100.0%	178.7 / NA	51	E78E (GAA→GAG)	pmrD	signal transduction protein PmrD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/27);  total (24/27)

CTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTC  >  NC_000913/2373171‑2373430                                                                                                                                       |                                                                                                                              cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          >  2:526450/1‑140 (MQ=255) cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          >  1:415796/1‑140 (MQ=255) cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          >  2:719297/1‑140 (MQ=255) cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          >  1:516887/1‑140 (MQ=255) cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTCTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          <  2:801434/140‑1 (MQ=255) cTTCCCGGCGGGGAAGGGATTTTTTCGCTAAAAGCACTACTGGGAGTAGCCTGTATTATGAGGGGTTGGATGCTGTTTTTCCTGCCCACGAAAAAACACCGTTACTGATTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          <  2:651838/140‑1 (MQ=255)       ggCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATc                                                                                                                    <  1:8888/140‑1 (MQ=255)         cTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCgg                                                                                                                  >  2:298958/1‑140 (MQ=255)         cTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTCCCTGCCCCCGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTTATCgg                                                                                                                  <  1:552787/140‑1 (MQ=255)                       tttCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCtt                                                                                                    <  1:77208/140‑1 (MQ=255)                       tttCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCtt                                                                                                    >  1:653620/1‑140 (MQ=255)                          cGCTGAAAGCACTCCTGGGAGAAGCCTGTATTATGAGCGGTTTGTTGCTGTTTTTCCTGCCCACAACAAAACAACGTTCCGGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGcc                                                                                                 <  2:393315/140‑1 (MQ=255)                          cGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGcc                                                                                                 <  2:655531/140‑1 (MQ=255)                             tGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCgag                                                                                              <  1:298958/140‑1 (MQ=255)                               aaaGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGagag                                                                                            <  1:526450/140‑1 (MQ=255)                                 aGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGa                                                                                          <  1:719297/140‑1 (MQ=255)                                           ggAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGt                                                                                >  1:609027/1‑140 (MQ=255)                                               tAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTc                                                                                     >  1:439968/1‑131 (MQ=255)                                               tAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTc                                                                                     <  2:439968/131‑1 (MQ=255)                                                  ccTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGc                                                                         >  2:243967/1‑140 (MQ=255)                                                     gTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCttt                                                                      >  1:447696/1‑140 (MQ=255)                                                      tattatGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCtttt                                                                     <  2:609027/140‑1 (MQ=255)                                                      tattatGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCtttt                                                                     >  1:685258/1‑140 (MQ=255)                                                          atGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACg                                                                 <  1:243967/140‑1 (MQ=255)                                                           tGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGa                                                                >  1:630352/1‑140 (MQ=255)                                                            gAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGAt                                                               <  2:516887/140‑1 (MQ=255)                                                            gAGCGGGTTGATGCTGATTTTCCTGCCCACGAAAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGAt                                                               <  2:415796/140‑1 (MQ=255)                                                                gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTc                                                                    >  1:522942/1‑131 (MQ=255)                                                                gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTc                                                                    <  2:522942/131‑1 (MQ=255)                                                                gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAAt                                                           >  2:679898/1‑140 (MQ=255)                                                                gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAAt                                                           >  2:677098/1‑140 (MQ=255)                                                                gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAAt                                                           >  1:660524/1‑140 (MQ=255)                                                                  gTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATAc                                                         >  2:189158/1‑140 (MQ=255)                                                                  gTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATAc                                                         >  1:583608/1‑140 (MQ=255)                                                                           gATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgcg                                                <  2:630352/140‑1 (MQ=255)                                                                            aTTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgcgc                                               >  2:751038/1‑140 (MQ=255)                                                                            aTTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgcgc                                               >  1:364865/1‑140 (MQ=255)                                                                             ttttCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCa                                              <  1:64072/140‑1 (MQ=255)                                                                             ttttCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCa                                              <  2:653620/140‑1 (MQ=255)                                                                             ttttCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCa                                              <  1:189158/140‑1 (MQ=255)                                                                                   tGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGc                                        <  1:679898/140‑1 (MQ=255)                                                                                    gCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCa                                       <  1:196853/140‑1 (MQ=255)                                                                                    gCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCa                                       <  2:660524/140‑1 (MQ=255)                                                                                         cGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGc                                  <  2:443134/140‑1 (MQ=255)                                                                                                    cGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATc                       >  1:406963/1‑140 (MQ=255)                                                                                                       tACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATcccc                    <  2:685258/140‑1 (MQ=255)                                                                                                         cTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCa                  >  2:680087/1‑140 (MQ=255)                                                                                                         cTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCa                  >  2:820440/1‑140 (MQ=255)                                                                                                              ttttCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTc             >  2:450498/1‑140 (MQ=255)                                                                                                                        cACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGt   <  2:406963/140‑1 (MQ=255)                                                                                                                         aCTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTc  <  2:563398/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                              CTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTC  >  NC_000913/2373171‑2373430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑