Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,377,547	A→G	W16R (TGG→CGG)	menH ←	2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,377,547	0	A	G	100.0%	154.2 / NA	44	W16R (TGG→CGG)	menH	2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/28);  total (16/28)

AAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCG  >  NC_000913/2377410‑2377680                                                                                                                                          |                                                                                                                                      aaaTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCCCTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCggg                                                                                                                                     <  1:280341/140‑1 (MQ=255) aaaTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCggg                                                                                                                                     <  1:374734/140‑1 (MQ=255)   aTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCggggg                                                                                                                                   >  1:301842/1‑139 (MQ=255)       aTCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAAc                                                                                                                               >  2:153108/1‑140 (MQ=255)        tCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAAcc                                                                                                                              <  1:854343/140‑1 (MQ=255)        tCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAG‑CGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCt                                                                                                                             >  1:260889/1‑140 (MQ=255)         cGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCt                                                                                                                             <  2:411180/140‑1 (MQ=255)          gACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTg                                                                                                                            <  2:261038/140‑1 (MQ=255)          gACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTg                                                                                                                            <  1:497920/140‑1 (MQ=255)                      cgccgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGtt                                                                                                                >  2:546418/1‑140 (MQ=255)                      cgccgAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGtt                                                                                                                <  1:546418/140‑1 (MQ=255)                           aaCCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGcc                                                                                                           >  1:580217/1‑140 (MQ=255)                               accGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTgcg                                                                                                       <  2:97735/140‑1 (MQ=255)                                   tGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTg                                                                                                   <  1:825069/140‑1 (MQ=255)                                   tGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTg                                                                                                   <  2:676868/140‑1 (MQ=255)                                     aCCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCa                                                                                                 <  1:500897/140‑1 (MQ=255)                                     aCCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCa                                                                                                 <  1:737038/140‑1 (MQ=255)                                     aCCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCa                                                                                                 <  1:230624/140‑1 (MQ=255)                                          ggAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATc                                                                                            >  1:836067/1‑140 (MQ=255)                                            agaTCAACATACAACCGTGAGTAG‑CGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATa                                                                                         <  2:260889/140‑1 (MQ=255)                                              aTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATaa                                                                                        <  2:434831/140‑1 (MQ=255)                                                 aaCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTg                                                                                                   <  1:586113/126‑1 (MQ=255)                                                 aaCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTg                                                                                                   >  2:586113/1‑126 (MQ=255)                                                        aaCCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAcc                                                                              <  1:72443/140‑1 (MQ=255)                                                         aCCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCt                                                                             >  1:861958/1‑140 (MQ=255)                                                          ccGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATa                                                                                         >  1:208196/1‑127 (MQ=255)                                                          ccGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATa                                                                                         <  2:208196/127‑1 (MQ=255)                                                             tGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgcgc                                                                         >  1:293901/1‑140 (MQ=255)                                                             tGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACC‑GCGcc                                                                        >  1:532614/1‑140 (MQ=255)                                                             tGAGTAGTCGGCAAACGCCTCGCCCACTTCTTCCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgcgc                                                                         >  1:441222/1‑140 (MQ=255)                                                              gAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTCGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGcc                                                                        >  2:253990/1‑140 (MQ=255)                                                                    tCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGt                                                                  >  2:1896/1‑140 (MQ=255)                                                                    tCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGt                                                                  >  1:92589/1‑140 (MQ=255)                                                                        cAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACC‑GCGCCAGAAGTTGCTg                                                             <  2:532614/140‑1 (MQ=255)                                                                                    ccACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgcg                                                  <  1:253990/140‑1 (MQ=255)                                                                                     cACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgcgc                                                 <  1:1896/140‑1 (MQ=255)                                                                                       cttcttGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCAc                                               >  1:687679/1‑140 (MQ=255)                                                                                         tcttGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCa                                             <  1:153108/140‑1 (MQ=255)                                                                                          cttGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCAt                                            <  2:861958/140‑1 (MQ=255)                                                                                          cttGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCACCAGAAGTTGCTGGAGCGTTTGCGCACCAt                                            <  2:100923/140‑1 (MQ=255)                                                                                                  tCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCg                                    >  2:861845/1‑140 (MQ=255)                                                                                                    gTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGtt                                  <  2:836067/140‑1 (MQ=255)                                                                                                       gCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAac                               <  2:293901/140‑1 (MQ=255)                                                                                                       gCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAac                               <  2:441222/140‑1 (MQ=255)                                                                                                         aaTCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAacca                             <  1:861845/140‑1 (MQ=255)                                                                                                         aaTCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTCAacca                             <  2:580217/140‑1 (MQ=255)                                                                                                           tCGCCGGAAAAACCGTGCAAAAACACCAGCCGGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAaccacc                           <  2:45280/140‑1 (MQ=255)                                                                                                                                    ccagccggGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCg  >  2:222158/1‑140 (MQ=255)                                                                                                                                    ccagccggGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCg  <  1:222158/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      AAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCG  >  NC_000913/2377410‑2377680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑