Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,391,857	C→T	T395T (ACG→ACA)	nuoM ←	NADH:quinone oxidoreductase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,391,857	0	C	T	100.0%	118.4 / NA	35	T395T (ACG→ACA)	nuoM	NADH:quinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/21);  total (14/21)

CGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGC  >  NC_000913/2391728‑2391985                                                                                                                                  |                                                                                                                                 cGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCg                                                                                                                        <  1:814389/140‑1 (MQ=255)   ccAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCa                                                                                                                      >  2:582345/1‑140 (MQ=255)    cAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCaa                                                                                                                     <  1:231491/140‑1 (MQ=255)    cAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCaa                                                                                                                     <  1:802477/140‑1 (MQ=255)        gAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGaa                                                                                                                 <  1:582345/140‑1 (MQ=255)              aCAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGa                                                                                                           <  1:584620/140‑1 (MQ=255)                         gACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCa                                                                                                <  2:139496/140‑1 (MQ=255)                          aCCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAg                                                                                               <  1:12286/140‑1 (MQ=255)                          aCCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTATTGCCACCGCAAAGaa                                                                                                                 >  2:81383/1‑122 (MQ=255)                          aCCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTATTGCCACCGCAAAGaa                                                                                                                 <  1:81383/122‑1 (MQ=255)                            cAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGcc                                                                                             >  1:427560/1‑140 (MQ=255)                                ccAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGG‑AGCTGCCGAAAAGAATCATAAATTCGCCGCCGAAGTTACCGGGGCCAGGCATCCCAAGGGTTGCCACCCCAAAGAACAGCGAAAGGGCTGCCGGCCATTTc                                                                                        >  2:483112/1‑140 (MQ=255)                                  aaaGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCa                                                                                       <  2:785732/140‑1 (MQ=255)                                  aaaGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCa                                                                                       <  2:829372/140‑1 (MQ=255)                                    aGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATc                                                                                     >  2:664828/1‑140 (MQ=255)                                         gagaTAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCt                                                                                >  2:82485/1‑140 (MQ=255)                                         gagaTAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCt                                                                                >  2:538725/1‑140 (MQ=255)                                         gagaTAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCt                                                                                >  2:528282/1‑140 (MQ=255)                                               aCGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAg                                                                          <  1:323470/140‑1 (MQ=255)                                                cGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGa                                                                         >  1:852107/1‑140 (MQ=255)                                                cGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGa                                                                         >  2:392930/1‑140 (MQ=255)                                                    aaTCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGc                                                                     <  2:499451/140‑1 (MQ=255)                                                     aTCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGcc                                                                    >  2:341349/1‑140 (MQ=255)                                                        aCCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGc                                                                     <  1:846590/136‑1 (MQ=255)                                                        aCCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGc                                                                     >  2:846590/1‑136 (MQ=255)                                                                aaCCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCa                                                         <  1:582672/140‑1 (MQ=255)                                                                      gAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTcgc                                                   <  1:528282/140‑1 (MQ=255)                                                                       aaGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTcgcg                                                  >  2:379074/1‑140 (MQ=255)                                                                               gAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGa                                          <  1:538725/140‑1 (MQ=255)                                                                                     aaTCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTa                                              <  2:312598/130‑1 (MQ=255)                                                                                     aaTCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTa                                              >  1:312598/1‑130 (MQ=255)                                                                                       tCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCa                                  <  1:664828/140‑1 (MQ=255)                                                                                       tCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCa                                  >  1:334303/1‑140 (MQ=255)                                                                                              ttCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCt                           <  1:379074/140‑1 (MQ=255)                                                                                                    gacgaAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACcac                     <  1:509857/140‑1 (MQ=255)                                                                                                                       ggCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACccgc  <  2:354113/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 CGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGC  >  NC_000913/2391728‑2391985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑