Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,437,716	C→T	V46I (GTC→ATC)	pdxB ←	erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,437,716	0	C	T	100.0%	134.4 / NA	39	V46I (GTC→ATC)	pdxB	erythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/20);  total (19/20)

CCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATCAACAAGGATTTT  >  NC_000913/2437586‑2437848                                                                                                                                   |                                                                                                                                     ccAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCAc                                                                                                                             >  2:682242/1‑140 (MQ=255) ccAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCAc                                                                                                                             >  1:268208/1‑140 (MQ=255) ccAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCAc                                                                                                                             >  2:660092/1‑140 (MQ=255)  cAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCAcc                                                                                                                            >  2:297234/1‑140 (MQ=255)  cAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCAcc                                                                                                                            <  1:443766/140‑1 (MQ=255)               aaaaCCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCcg                                                                                                               >  1:147251/1‑140 (MQ=255)                aaaCCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGGGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCcgc                                                                                                              >  1:676036/1‑140 (MQ=255)                      aTTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGc                                                                                                        <  1:846808/140‑1 (MQ=255)                          ccGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGt                                                                                                    <  2:856842/140‑1 (MQ=255)                                 cTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGa                                                                                             <  1:297234/140‑1 (MQ=255)                                 cTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGa                                                                                             <  1:682242/140‑1 (MQ=255)                                  ttCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGAc                                                                                            <  2:268208/140‑1 (MQ=255)                                  ttCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGAc                                                                                            <  2:258707/140‑1 (MQ=255)                                     aaCCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACggg                                                                                         <  2:323593/140‑1 (MQ=255)                                           gCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGa                                                                                                 >  1:138943/1‑126 (MQ=255)                                           gCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGa                                                                                                 <  2:138943/126‑1 (MQ=255)                                            cTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGa                                                                                  <  2:428165/140‑1 (MQ=255)                                            cTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGa                                                                                  >  1:492645/1‑140 (MQ=255)                                            cTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGa                                                                                  <  2:188037/140‑1 (MQ=255)                                                 tCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGa                                                                                  <  1:462124/135‑1 (MQ=255)                                                 tCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGa                                                                                  >  2:462124/1‑135 (MQ=255)                                                         gtcggtcCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACcg                                                                     >  2:790483/1‑140 (MQ=255)                                                         gtcggtcCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACcg                                                                     >  2:632677/1‑140 (MQ=255)                                                           cggtcCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACcgcg                                                                   <  2:380793/140‑1 (MQ=255)                                                            ggtcCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCgg                                                                  <  2:492645/140‑1 (MQ=255)                                                              tcCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGtc                                                                >  1:212879/1‑140 (MQ=255)                                                                cccGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGtcac                                                              <  1:790483/140‑1 (MQ=255)                                                                                            tGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCgg                                  >  1:285337/1‑140 (MQ=255)                                                                                                ttttCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCAt                              <  1:632677/140‑1 (MQ=255)                                                                                                ttttCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCAt                              <  2:147251/140‑1 (MQ=255)                                                                                                  ttCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATaa                            >  1:655330/1‑140 (MQ=255)                                                                                                  ttCCTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATaa                            >  2:744021/1‑140 (MQ=255)                                                                                                     cTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGc                         <  2:655330/140‑1 (MQ=255)                                                                                                     cTGCCAGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACCCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGc                         <  2:608181/140‑1 (MQ=255)                                                                                                          aGCAAAGATTCATTCACTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATAtt                    >  1:707102/1‑140 (MQ=255)                                                                                                                     attcaCTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATcaacaa         >  2:636300/1‑140 (MQ=255)                                                                                                                      ttcaCTTTCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATCAACAAg        >  1:803704/1‑140 (MQ=255)                                                                                                                            ttCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATCAACAAGGAtttt  >  2:268724/1‑140 (MQ=255)                                                                                                                            ttCGTGATCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATCAACAAGGAtttt  <  1:387707/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                     CCAGGTGCAGCGGAAAAACCAATTCCCGCCTGCTTCAACCATGCTTCATCGACATGGTCGGTCCCCGCAGTGGCAGTGCCAACAAATTTAATGGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATCAACAAGGATTTT  >  NC_000913/2437586‑2437848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑