Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,252	A→G	V170A (GTA→GCA)	dapA ←	4‑hydroxy‑tetrahydrodipicolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,252	0	A	G	100.0%	129.5 / NA	38	V170A (GTA→GCA)	dapA	4‑hydroxy‑tetrahydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/21);  total (17/21)

GGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAT  >  NC_000913/2599116‑2599387                                                                                                                                         |                                                                                                                                        ggCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTgcac                                                                                                                                      >  2:315957/1‑140 (MQ=255)     atatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGt                                                                                                                                  <  1:805246/140‑1 (MQ=255)      tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGtt                                                                                                                                 <  1:778761/140‑1 (MQ=255)      tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGtt                                                                                                                                 >  2:833365/1‑140 (MQ=255)           cgcgCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTgcac                                                                                                                                      <  2:303419/130‑1 (MQ=255)           cgcgCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTgcac                                                                                                                                      >  1:303419/1‑130 (MQ=255)           cgcgCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGtt                                                                                                                            >  1:493792/1‑140 (MQ=255)                tGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTg                                                                                                                       >  2:498918/1‑140 (MQ=255)                  cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGtt                                                                                                                     >  1:609356/1‑140 (MQ=255)                    aCGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGc                                                                                                                   <  2:461433/140‑1 (MQ=255)                        tAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCctct                                                                                                               <  1:62156/140‑1 (MQ=255)                             gTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGAt                                                                                                          <  2:852445/140‑1 (MQ=255)                             gTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGAt                                                                                                          <  2:493792/140‑1 (MQ=255)                              tAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGAtt                                                                                                         >  2:10731/1‑140 (MQ=255)                                         aCCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtt                                                                                              >  1:356706/1‑140 (MQ=255)                                         aCCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtt                                                                                              >  1:344705/1‑140 (MQ=255)                                          ccccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAttt                                                                                             >  2:688145/1‑140 (MQ=255)                                           cccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttt                                                                                            <  1:315957/140‑1 (MQ=255)                                              aTGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTa                                                                                         >  2:673088/1‑140 (MQ=255)                                                    gCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCg                                                                                   >  1:309440/1‑140 (MQ=255)                                                     cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                                                  >  1:698335/1‑140 (MQ=255)                                                         aTTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGa                                                                              <  2:356706/140‑1 (MQ=255)                                                         aTTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGa                                                                              <  2:344705/140‑1 (MQ=255)                                                                       aGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTc                                                                <  1:22374/140‑1 (MQ=255)                                                                         cgcgcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCg                                                              >  2:807801/1‑140 (MQ=255)                                                                              tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGc                                                         >  1:660448/1‑140 (MQ=255)                                                                                      tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCa                                                 >  2:18284/1‑140 (MQ=255)                                                                                        gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                                               <  1:734980/140‑1 (MQ=255)                                                                                        gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                                               <  1:722409/140‑1 (MQ=255)                                                                                        gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                                               <  1:688145/140‑1 (MQ=255)                                                                                                  agAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                     <  1:673088/140‑1 (MQ=255)                                                                                                    aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                                   <  1:807801/140‑1 (MQ=255)                                                                                                              tcTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtata                         >  2:514869/1‑140 (MQ=255)                                                                                                                   aaCCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGaa                    >  2:748654/1‑140 (MQ=255)                                                                                                                          tctTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCgg             <  2:660448/140‑1 (MQ=255)                                                                                                                            tttGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa           <  2:167714/140‑1 (MQ=255)                                                                                                                             ttGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAg          <  1:10731/140‑1 (MQ=255)                                                                                                                              tgattcggTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTAATTCCGATAATAGTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCTAGTACGGGACGGCACATTATACAGAATTTGCGGCAgg         <  2:766464/134‑1 (MQ=255)                                                                                                                              tGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAgg         <  1:514869/140‑1 (MQ=255)                                                                                                                               gATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGt        <  1:833365/140‑1 (MQ=255)                                                                                                                                     gTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAt  >  2:471221/1‑140 (MQ=255)                                                                                                                                      tttGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCa      <  1:482667/135‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAT  >  NC_000913/2599116‑2599387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑