Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	223,484	C→T	intergenic (+76/‑287)	gmhB → / → rrsH	D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	223,484	0	C	T	100.0%	187.0 / NA	55	intergenic (+76/‑287)	gmhB/rrsH	D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/28);  total (27/28)

TAAATAGCCTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGT  >  NC_000913/223350‑223620                                                                                                                                       |                                                                                                                                         tAAATAGCCTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGa                                                                                                                                     >  1:674453/1‑140 (MQ=255)      aGCCTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACAcgg                                                                                                                                <  2:749207/140‑1 (MQ=255)       gCCTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACAcggc                                                                                                                               <  2:34411/140‑1 (MQ=255)        ccTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACAcggcg                                                                                                                              >  1:621788/1‑140 (MQ=255)         cTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACAcggcgg                                                                                                                             <  2:106236/140‑1 (MQ=255)         cTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACAcggcgg                                                                                                                             <  2:101781/140‑1 (MQ=255)         cTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACAcggcgg                                                                                                                             >  1:355242/1‑140 (MQ=255)          tGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGa                                                                                                                            >  2:20110/1‑140 (MQ=255)            gCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGAtg                                                                                                                          >  1:486877/1‑140 (MQ=255)             cAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGAtgt                                                                                                                         >  1:560093/1‑140 (MQ=255)                 ccTGCCGCAAGCGAT‑AAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATc                                                                                                                    >  1:502385/1‑140 (MQ=255)                  cTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATc                                                                                                                    >  2:261445/1‑140 (MQ=255)                   tGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCa                                                                                                                   <  1:238547/140‑1 (MQ=255)                     ccGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACt                                                                                                                 >  2:520612/1‑140 (MQ=255)                            cGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTcacaca                                                                                                          <  1:287208/140‑1 (MQ=255)                                  aaaaGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGc                                                                                                    <  1:391459/140‑1 (MQ=255)                                  aaaaGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGc                                                                                                    <  1:25718/140‑1 (MQ=255)                                   aaaGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGcc                                                                                                   >  2:83224/1‑140 (MQ=255)                                     agcagcAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcgg                                                                                                 >  2:593374/1‑140 (MQ=255)                                      gcagcaAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggt                                                                                                >  1:364354/1‑140 (MQ=255)                                       cagcaAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggtt                                                                                               <  1:593374/140‑1 (MQ=255)                                       cagcaAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggtt                                                                                               <  2:364354/140‑1 (MQ=255)                                          caAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcgg                                                                                            <  2:733652/140‑1 (MQ=255)                                                    cacaATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaa                                                                                  <  2:502385/140‑1 (MQ=255)                                                      caATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaaaa                                                                                <  2:355242/140‑1 (MQ=255)                                                         tGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATc                                                                             >  1:638495/1‑140 (MQ=255)                                                         tGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATc                                                                             >  1:239986/1‑140 (MQ=255)                                                            ttAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTg                                                                          <  2:674453/140‑1 (MQ=255)                                                            ttAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTg                                                                          <  1:20110/140‑1 (MQ=255)                                                                 aGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAAtt                                                                     >  2:497029/1‑140 (MQ=255)                                                                 aGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAAtt                                                                     >  1:350395/1‑140 (MQ=255)                                                                     gAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAgg                                                                 >  2:746844/1‑140 (MQ=255)                                                                      aGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAggg                                                                >  2:842530/1‑140 (MQ=255)                                                                       gCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGCCTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAACCCTGAAATTCAGGGt                                                               <  1:746844/140‑1 (MQ=255)                                                                       gCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGt                                                               <  2:486877/140‑1 (MQ=255)                                                                           ttGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGAc                                                           >  1:231052/1‑140 (MQ=255)                                                                           ttGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGAc                                                           >  1:386787/1‑140 (MQ=255)                                                                            tGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGAct                                                          <  1:643853/140‑1 (MQ=255)                                                                             gAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGActc                                                         >  2:758151/1‑140 (MQ=255)                                                                                    aaaTGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAaga                                                  >  1:864829/1‑140 (MQ=255)                                                                                       tGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGa                                               <  1:750067/140‑1 (MQ=255)                                                                                       tGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGa                                               <  1:267711/140‑1 (MQ=255)                                                                                            ttttCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCg                                          <  2:231052/140‑1 (MQ=255)                                                                                            ttttCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCg                                          <  1:261445/140‑1 (MQ=255)                                                                                            ttttCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCg                                          <  2:386787/140‑1 (MQ=255)                                                                                            ttttCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCg                                          <  1:332138/140‑1 (MQ=255)                                                                                            tttaCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCg                                          <  2:595286/140‑1 (MQ=255)                                                                                                                gCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGc                                <  2:613535/130‑1 (MQ=18)                                                                                                                gCCGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAACCGTAATATACGc                                >  1:613535/1‑130 (MQ=16)                                                                                                                 ccGACTCCCTATAATGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAACAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGAc                     <  1:758151/140‑1 (MQ=17)                                                                                                                               tGCGCCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGc       <  1:842530/140‑1 (MQ=38)                                                                                                                                 cgcCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCcg     >  2:395718/1‑140 (MQ=255)                                                                                                                                  gcCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCcgc    >  1:335337/1‑140 (MQ=255)                                                                                                                                  gcCTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCcgc    >  1:408081/1‑140 (MQ=255)                                                                                                                                   ccTCTATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCcgcg   >  1:148192/1‑140 (MQ=255)                                                                                                                                    ctctATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGt  <  2:239986/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TAAATAGCCTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCAAAAACCGGCACAATGATTAAAAGATGAGCGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGT  >  NC_000913/223350‑223620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑