Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,417	G→A	A865A (GCC→GCT)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,417	0	G	A	100.0%	238.1 / NA	67	A865A (GCC→GCT)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/38);  total (29/38)

AAGTTTAGCCGATATACACAACTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTGGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCC  >  NC_000913/2819280‑2819552                                                                                                                                          |                                                                                                                                        aaGTTTAGCCGATATACACAACTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGc                                                                                                                                       >  2:327281/1‑140 (MQ=255)        gCCGATATACACAACTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCt                                                                                                                                >  1:512928/1‑140 (MQ=255)         ccGATATACACAACTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTg                                                                                                                               >  2:297821/1‑140 (MQ=255)         ccGATATACACAACTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTg                                                                                                                               <  2:831457/140‑1 (MQ=255)                      cTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgc                                                                                                                  <  1:588352/140‑1 (MQ=255)                      cTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgc                                                                                                                  >  2:191976/1‑140 (MQ=255)                      cTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgc                                                                                                                  >  2:439275/1‑140 (MQ=255)                      cTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgc                                                                                                                  >  2:33706/1‑140 (MQ=255)                      cTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgc                                                                                                                  >  2:783148/1‑140 (MQ=255)                      cTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgc                                                                                                                  >  1:383836/1‑140 (MQ=255)                       ttCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAgg                                                                                                                           >  2:384280/1‑130 (MQ=255)                       ttCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAgg                                                                                                                           <  1:384280/130‑1 (MQ=255)                        tCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgcag                                                                                                                >  1:187769/1‑140 (MQ=255)                         cAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgcagc                                                                                                               <  2:242037/140‑1 (MQ=255)                         cAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgcagc                                                                                                               <  1:318071/140‑1 (MQ=255)                           aCCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCAt                                                                                                             >  1:709220/1‑140 (MQ=255)                           aCCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCAt                                                                                                             <  2:549008/140‑1 (MQ=255)                            ccTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATc                                                                                                            <  1:464779/140‑1 (MQ=255)                             cTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATcc                                                                                                           <  2:846897/140‑1 (MQ=255)                              tGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCCATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCg                                                                                                          <  2:141925/140‑1 (MQ=255)                                aCTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgca                                                                                                                 <  1:259635/131‑1 (MQ=255)                                aCTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCgca                                                                                                                 >  2:259635/1‑131 (MQ=255)                                aCTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTa                                                                                                        >  2:178134/1‑140 (MQ=255)                                   ttATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTacca                                                                                                     <  1:297821/140‑1 (MQ=255)                                    tATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTaccac                                                                                                    <  2:703419/140‑1 (MQ=255)                                           gTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTg                                                                                             >  1:858826/1‑140 (MQ=255)                                             cGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTgcg                                                                                           <  1:178134/140‑1 (MQ=255)                                             cGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTgcg                                                                                           <  2:249446/140‑1 (MQ=255)                                             cGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTgcg                                                                                           <  1:191976/140‑1 (MQ=255)                                                    gTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTc                                                                                    <  2:181606/140‑1 (MQ=255)                                                      tAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAg                                                                                  >  1:91987/1‑140 (MQ=255)                                                        aTGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGa                                                                                <  1:33706/140‑1 (MQ=255)                                                             aaTGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTcc                                                                           >  2:383355/1‑140 (MQ=255)                                                             aaTGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTcc                                                                           >  2:163432/1‑140 (MQ=255)                                                               tGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTccac                                                                         >  1:28784/1‑140 (MQ=255)                                                                    tGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCAccc                                                                    >  1:414027/1‑140 (MQ=255)                                                                    tGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCAccc                                                                    >  1:230477/1‑140 (MQ=255)                                                                        gCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTgc                                                                <  2:646830/140‑1 (MQ=255)                                                                            gTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGTATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTgccgcc                                                            <  1:45220/140‑1 (MQ=255)                                                                            gTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTgccgcc                                                            >  1:614059/1‑140 (MQ=255)                                                                            gTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTgccgcc                                                            <  2:379587/140‑1 (MQ=255)                                                                                      ttGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCCCCCTTGCCGCCCACCTGCTGa                                                  <  2:614059/140‑1 (MQ=255)                                                                                      ttGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGa                                                  <  2:512928/140‑1 (MQ=255)                                                                                       tGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAg                                                 <  2:612702/140‑1 (MQ=255)                                                                                         ccTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCg                                               >  2:510507/1‑140 (MQ=255)                                                                                          cTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGa                                              <  2:383836/140‑1 (MQ=255)                                                                                            gACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGTCGCCCACCTGCTGAGCGAcc                                            <  2:91987/140‑1 (MQ=255)                                                                                              cGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCAt                                          >  1:347456/1‑140 (MQ=255)                                                                                                        attGCAATTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGt                                >  1:365371/1‑140 (MQ=255)                                                                                                             aaTTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTcccc                           <  2:347456/140‑1 (MQ=255)                                                                                                              aTTTCGCGCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCt                          >  2:707465/1‑140 (MQ=255)                                                                                                                  cgcgCTGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCtt                      >  1:630435/1‑140 (MQ=255)                                                                                                                       tGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTcaca                 <  2:414027/140‑1 (MQ=255)                                                                                                                       tGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTcaca                 <  2:858826/140‑1 (MQ=255)                                                                                                                       tGACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTcaca                 <  2:709220/140‑1 (MQ=255)                                                                                                                        gACCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCacac                <  1:180520/140‑1 (MQ=255)                                                                                                                         aCCCAGCCTTTCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACg               >  1:866821/1‑140 (MQ=255)                                                                                                                                 tttCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGa       <  1:383355/140‑1 (MQ=255)                                                                                                                                 tttCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGa       <  2:214368/140‑1 (MQ=255)                                                                                                                                  ttCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGAc      <  1:510507/140‑1 (MQ=255)                                                                                                                                  ttCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGAc      <  2:365371/140‑1 (MQ=255)                                                                                                                                   tCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTcaca                 <  2:260145/128‑1 (MQ=255)                                                                                                                                   tCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTcaca                 >  1:260145/1‑128 (MQ=255)                                                                                                                                   tCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGAc      <  1:604435/139‑1 (MQ=255)                                                                                                                                   tCACACTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGAc      >  2:604435/1‑139 (MQ=255)                                                                                                                                    cacaCTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGt    <  2:866821/140‑1 (MQ=255)                                                                                                                                      cacTAGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTcc  <  2:28784/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AAGTTTAGCCGATATACACAACTTCAACCTGACTTTATCGTTGTCGATAGCGTTAATGCGAATGCCGTGAAGCGAGTCCACGGCATTGCCTGACGCTTATATTATTGCAATTTCGCGCTGACCCAGCCTTTCACACTGGCTAACGCTGCAGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCC  >  NC_000913/2819280‑2819552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑