Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,826,251	C→T	G140G (GGC→GGT)	srlA →	sorbitol‑specific PTS enzyme IIC2 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,826,251	0	C	T	100.0%	97.4 / NA	29	G140G (GGC→GGT)	srlA	sorbitol‑specific PTS enzyme IIC2 component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/13);  total (16/13)

ACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGG  >  NC_000913/2826119‑2826382                                                                                                                                     |                                                                                                                                    aCCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTTCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTgg                                                                                                                              >  2:803721/1‑140 (MQ=255) aCCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTgg                                                                                                                              >  2:411374/1‑140 (MQ=255)   ccAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCg                                                                                                                            <  1:691118/140‑1 (MQ=255)    cAGCTACTACGCGGGGGCCTTTTATACTTGCCACTCAATAAATGGCCTCTTCCCCCAAATCACCCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGGGGTCTGACAACGCAGAACCTGCCACTTGGTCCACTGGCgg                                                                                                                           <  2:866416/140‑1 (MQ=255)      gCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGt                                                                                                                          <  2:450691/139‑1 (MQ=255)      gCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGt                                                                                                                          >  1:450691/1‑139 (MQ=255)               cggcggCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATctg                                                                                                                >  1:275788/1‑140 (MQ=255)                 gcggcCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATctgct                                                                                                              <  2:275788/140‑1 (MQ=255)                        ctTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCtggttggt                                                                                                       >  1:365713/1‑140 (MQ=255)                         ttATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTtggtc                                                                                                      >  2:73853/1‑140 (MQ=255)                           ataGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTtggtctg                                                                                                    <  1:411374/140‑1 (MQ=255)                                 gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCtgg                                                                                                            <  2:727899/126‑1 (MQ=255)                                 gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCtgg                                                                                                            >  1:727899/1‑126 (MQ=255)                                 gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCa                                                                                                <  1:631657/138‑1 (MQ=255)                                 gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCa                                                                                                >  2:631657/1‑138 (MQ=255)                                  ccACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGGGAGGTATCTGCTGGTTGGGCTGGTCCCCa                                                                                             >  2:169332/1‑140 (MQ=255)                                   cACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGGGGTTTCTGCTGGTTGGTCTGGTCACCaa                                                                                            >  2:429778/1‑140 (MQ=255)                                          tgaatgGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGCTATCTTCTTGTTGGTCTCGTTCCCCATttcttc                                                                                     >  2:546339/1‑140 (MQ=255)                                             atgGCCTCTTCCCCCATATCACCCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCcgc                                                                                  <  1:295699/140‑1 (MQ=255)                                                gCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGc                                                                               <  2:820748/140‑1 (MQ=255)                                                    ctTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGGTATCTTCTGGGTGGTCTGGTCACCAATTTCTTTCGCGGCTggg                                                                           >  1:561887/1‑140 (MQ=255)                                                      cagcccATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTg                                                                         <  1:676829/137‑1 (MQ=255)                                                       cccccATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGa                                                                        <  1:73853/140‑1 (MQ=255)                                                       cccccATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGa                                                                        <  1:429778/140‑1 (MQ=255)                                                                    ccTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGGGTTATTTGCTGGTTGGTCTGGTCACCAATTTTTTCCGCGGCTGGGTGACCGATCTGacctg                                                           >  2:676829/1‑138 (MQ=255)                                                                        gCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGcc                                                       >  1:793431/1‑140 (MQ=255)                                                                                         tttGGCATTGCCAGCGGTCTGACACCGCTGACCCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGAt                                      <  1:803721/139‑1 (MQ=255)                                                                                                             gACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGACCGATCTGACCACCGCCATTTTTTAGAAAAAGATGGGCATTCAACTTGaaaaaa                  >  1:402215/1‑140 (MQ=255)                                                                                                                             ccACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGACCGATCTGACCACCGCCATTTTTTAGAAAAAAATGGGCATTCAACTTGAACAAAAAATTCACCTGGCAgg  >  2:400994/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                    ACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGG  >  NC_000913/2826119‑2826382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑