Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,018,115	G→A	A686T (GCG→ACG)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,018,115	0	G	A	100.0%	155.4 / NA	45	A686T (GCG→ACG)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/23);  total (22/23)

GCCACCGGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACG  >  NC_000913/3017980‑3018247                                                                                                                                        |                                                                                                                                     gCCACCGGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACggg                                                                                                                                  >  1:95910/1‑140 (MQ=255) gCCACCGGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACggg                                                                                                                                  >  1:352308/1‑140 (MQ=255)  ccACCGGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGt                                                                                                                                 <  1:7980/140‑1 (MQ=255)   cACCGGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTa                                                                                                                                >  2:751633/1‑140 (MQ=255)      cGGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAaca                                                                                                                             <  2:25073/140‑1 (MQ=255)         cACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACAccc                                                                                                                          >  2:427117/1‑139 (MQ=255)                     tAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTgcg                                                                                                              <  2:465216/140‑1 (MQ=255)                        cGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTc                                                                                                           >  1:308339/1‑140 (MQ=255)                                     gcgggcgACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTa                                                                                                >  1:22017/1‑138 (MQ=255)                                     gcgggcgACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTa                                                                                                <  2:22017/138‑1 (MQ=255)                                     gcgggcgACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTAcg                                                                                              <  2:308339/140‑1 (MQ=255)                                          cgACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTc                                                                                         >  1:729622/1‑140 (MQ=255)                                               aCCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGc                                                                                    <  2:652454/140‑1 (MQ=255)                                                   aaaTGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAg                                                                                >  2:330261/1‑140 (MQ=255)                                                     aTGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGaa                                                                              <  2:729622/140‑1 (MQ=255)                                                          tGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGc                                                                         >  2:571823/1‑140 (MQ=255)                                                          tGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGc                                                                         >  2:109297/1‑140 (MQ=255)                                                           ggAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCa                                                                        >  1:218111/1‑140 (MQ=255)                                                            gAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCaa                                                                       <  2:85147/140‑1 (MQ=255)                                                                         ttCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTAc                                                            >  1:697964/1‑138 (MQ=255)                                                                         ttCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTAc                                                            <  2:697964/138‑1 (MQ=255)                                                                         ttCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACcg                                                          <  2:218111/140‑1 (MQ=255)                                                                            cTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACcgtcg                                                       <  2:54139/140‑1 (MQ=255)                                                                              gCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGtt                                                     <  1:751633/140‑1 (MQ=255)                                                                              gCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGtt                                                     <  2:298732/140‑1 (MQ=255)                                                                              gCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGtt                                                     <  1:540828/140‑1 (MQ=255)                                                                                gTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCa                                                   >  1:805926/1‑140 (MQ=255)                                                                                gTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCa                                                   >  1:724681/1‑140 (MQ=255)                                                                                   aaTACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTa                                                <  2:724681/140‑1 (MQ=255)                                                                                        aacaaGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAaga                                           <  2:805926/140‑1 (MQ=255)                                                                                             gggTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGc                                      >  1:674989/1‑140 (MQ=255)                                                                                              ggTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGcc                                     >  2:189615/1‑140 (MQ=255)                                                                                                tACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCg                                   >  1:33021/1‑140 (MQ=255)                                                                                                 aCAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGc                                  <  2:198888/140‑1 (MQ=255)                                                                                                  cAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCa                                 <  2:33021/140‑1 (MQ=255)                                                                                                     cgcTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATgg                              >  1:574467/1‑140 (MQ=255)                                                                                                        tCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATGgcgc                           >  2:519793/1‑140 (MQ=255)                                                                                                        tCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATGgcgc                           >  1:580805/1‑140 (MQ=255)                                                                                                        tCAAGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATGgcgc                           <  1:571823/140‑1 (MQ=255)                                                                                                           aGCTGGGCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATGGCGCGaa                        <  2:674989/140‑1 (MQ=255)                                                                                                                 gCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGc                                  >  2:162100/1‑124 (MQ=255)                                                                                                                 gCAAACATGTGGTCGTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGc                                  <  1:162100/124‑1 (MQ=255)                                                                                                                               gTTGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCa    <  1:109297/140‑1 (MQ=255)                                                                                                                                 tGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCacg  >  2:231162/1‑140 (MQ=255)                                                                                                                                 tGTCGGGACGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCacg  <  1:330261/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     GCCACCGGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCGGGTAACACCGCAATGGACTGCGCTCGTGCGGCGTTACGCGTTCCAGGCGTAGAAAAAGCAACGATCGTTTACCGTCGTTCACTACAAGAGATGCCCGCATGGCGCGAAGAGTATGAAGAAGCGTTGCACG  >  NC_000913/3017980‑3018247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑