Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,121,914	T→C	D631G (GAC→GGC)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,121,914	0	T	C	100.0%	193.8 / NA	55	D631G (GAC→GGC)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/26);  total (29/26)

ATCGCCAGCGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCC  >  NC_000913/3121784‑3122042                                                                                                                                   |                                                                                                                                 aTCGCCAGCGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCa                                                                                                                         >  1:589926/1‑140 (MQ=255)    gCCAGCGTTTTGCTGATCAACCACTTTCGCCATCTTCTCCAGCGACGCCTGCACCTGTTCTTTGTTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACg                                                                                                                      <  2:689694/140‑1 (MQ=255)    gCCAGCGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACg                                                                                                                      <  2:247037/140‑1 (MQ=255)     ccAGCGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGc                                                                                                                     <  2:61930/140‑1 (MQ=255)     ccAGCGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGc                                                                                                                     <  1:509025/140‑1 (MQ=255)      cAGCGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGcc                                                                                                                    <  1:109992/140‑1 (MQ=255)      cAGCGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGcc                                                                                                                    <  2:118347/140‑1 (MQ=255)       aGCGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCca                                                                                                                   >  1:828096/1‑140 (MQ=255)         cGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCcaca                                                                                                                 <  1:554600/140‑1 (MQ=255)              tGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCAt                                                                                                                           <  1:309031/125‑1 (MQ=255)              tGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCAt                                                                                                                           >  2:309031/1‑125 (MQ=255)              tGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATtgtg                                                                                                            >  2:580019/1‑140 (MQ=255)                       aCCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCgg                                                                                                   <  2:395469/140‑1 (MQ=255)                       aCCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCgg                                                                                                   <  1:580019/140‑1 (MQ=255)                             ttCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACttt                                                                                             >  1:692674/1‑140 (MQ=255)                             ttCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACttt                                                                                             >  2:398633/1‑140 (MQ=255)                             ttCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACttt                                                                                             >  1:53146/1‑140 (MQ=255)                                 ccATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGaa                                                                                         <  2:589926/140‑1 (MQ=255)                                     atTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGaacaac                                                                                     >  1:171872/1‑140 (MQ=255)                                            aGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATccc                                                                              <  2:53146/140‑1 (MQ=255)                                              cGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCt                                                                            >  1:690202/1‑140 (MQ=255)                                                aCGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGc                                                                          >  2:27229/1‑140 (MQ=255)                                                aCGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGc                                                                          >  1:859830/1‑140 (MQ=255)                                                  gCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTc                                                                        >  2:519816/1‑140 (MQ=255)                                                   ccTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTcc                                                                       >  2:355957/1‑140 (MQ=255)                                                       cACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCAccc                                                                   >  1:485710/1‑140 (MQ=255)                                                             ttCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGca                                                             <  2:171872/140‑1 (MQ=255)                                                               cTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGcacc                                                           <  2:327019/140‑1 (MQ=255)                                                                      cAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAATGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTAcc                                                    <  2:787874/140‑1 (MQ=255)                                                                              cGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATc                                            >  1:81920/1‑140 (MQ=255)                                                                              cGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATc                                            >  1:76730/1‑140 (MQ=255)                                                                              cGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATc                                            >  2:528225/1‑140 (MQ=255)                                                                                tGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATccc                                          <  1:519816/140‑1 (MQ=255)                                                                                   cGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATccc                                          <  1:411271/137‑1 (MQ=255)                                                                                   cGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATccc                                          >  2:411271/1‑137 (MQ=255)                                                                                   cGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTg                                       >  1:175799/1‑140 (MQ=255)                                                                                   cGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTg                                       >  1:143895/1‑140 (MQ=255)                                                                                     tAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCa                                     <  1:27229/140‑1 (MQ=255)                                                                                     tAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCa                                     <  1:355957/140‑1 (MQ=255)                                                                                     tAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCa                                     <  2:692674/140‑1 (MQ=255)                                                                                     tAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCa                                     <  1:586967/140‑1 (MQ=255)                                                                                              ggCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTAt                            >  1:201538/1‑140 (MQ=255)                                                                                                cGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATcc                          <  2:828096/140‑1 (MQ=255)                                                                                                cGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATcc                          <  2:201538/140‑1 (MQ=255)                                                                                                  atatGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAg                        >  2:409277/1‑140 (MQ=255)                                                                                                     tGCTGGCTGGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGctc                     <  2:81920/140‑1 (MQ=255)                                                                                                      gctggctgGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGctct                    >  1:285262/1‑140 (MQ=255)                                                                                                      gctggctgGAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGctct                    >  2:47627/1‑140 (MQ=255)                                                                                                          gctggAGATACGCAGCATTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCACACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCttgtt                >  1:133985/1‑140 (MQ=255)                                                                                                           ctggAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCttgttg               >  2:184805/1‑140 (MQ=255)                                                                                                            tggAGATACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTgg              <  2:490985/140‑1 (MQ=255)                                                                                                             ggAGATACGCAGCGTTGCGCGGCCTTCCGTCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGa             >  2:262675/1‑140 (MQ=255)                                                                                                               agaTACGCAGCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGAtc           >  2:857132/1‑140 (MQ=255)                                                                                                                 aTACGCAGCGTTGCGCGGCCTTCCGTCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGAtctc         <  1:262675/140‑1 (MQ=255)                                                                                                                        gCGTTGCGCGGCCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGcc  >  2:598034/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                 ATCGCCAGCGTTTTGCTGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTTGAACAACCAATCCCCTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCC  >  NC_000913/3121784‑3122042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑