Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,177,350	G→A	N187N (AAC→AAT)	nudF ←	ADP‑sugar pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,350	0	G	A	100.0%	129.2 / NA	37	N187N (AAC→AAT)	nudF	ADP‑sugar pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/24);  total (13/24)

CGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCC  >  NC_000913/3177213‑3177476                                                                                                                                          |                                                                                                                               cgcAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCAtt                                                                                                                              >  2:65849/1‑140 (MQ=255)   cAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGt                                                                                                                            <  2:558168/140‑1 (MQ=255)             aaGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTccc                                                                                                                  <  1:492749/140‑1 (MQ=255)                ttCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCctc                                                                                                               <  1:53337/140‑1 (MQ=255)                  cATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtctt                                                                                                             <  2:532105/140‑1 (MQ=255)                  cATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtctt                                                                                                             <  2:50906/140‑1 (MQ=255)                  cATCTCGCACAGGAGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtctt                                                                                                             <  1:326268/140‑1 (MQ=255)                   aTCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtcttc                                                                                                            >  1:660409/1‑140 (MQ=255)                    tctcGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtcttct                                                                                                           <  1:65849/140‑1 (MQ=255)                    tctcGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtcttct                                                                                                           <  2:660409/140‑1 (MQ=255)                       cGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTAcc                                                                                                        <  1:801737/140‑1 (MQ=255)                        gCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTAccc                                                                                                       <  1:576696/140‑1 (MQ=255)                          acaGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCAc                                                                                                     <  2:113103/140‑1 (MQ=255)                              gcgcATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGt                                                                                                 >  2:34278/1‑140 (MQ=255)                                  atcatTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGc                                                                                             <  1:18387/140‑1 (MQ=255)                                              aaGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTc                                                                                       >  2:661918/1‑134 (MQ=255)                                              aaGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTc                                                                                       <  1:661918/134‑1 (MQ=255)                                              aaGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCt                                                                                 <  1:5719/140‑1 (MQ=255)                                                gTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGTCCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTa                                                                               <  1:70628/140‑1 (MQ=255)                                                               cTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGaa                                                                >  1:182850/1‑140 (MQ=255)                                                                  cATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAatat                                                             >  1:469345/1‑140 (MQ=255)                                                                        tGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGt                                                       >  1:446847/1‑140 (MQ=255)                                                                          cccACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGttt                                                     >  2:140851/1‑140 (MQ=255)                                                                             aCTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTtca                                                  >  1:498504/1‑140 (MQ=255)                                                                              cTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAatat                                                             <  1:499674/128‑1 (MQ=255)                                                                              cTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAatat                                                             >  2:499674/1‑128 (MQ=255)                                                                              cTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTtcat                                                 >  2:481554/1‑140 (MQ=255)                                                                                      ttAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGa                                         <  2:182850/140‑1 (MQ=255)                                                                                       tAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGAc                                        <  2:469345/140‑1 (MQ=255)                                                                                        aaCGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGAcc                                       <  1:481554/140‑1 (MQ=255)                                                                                             ttGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGaa                                  <  2:446847/140‑1 (MQ=255)                                                                                                      cagctgcagcCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTg                         <  1:34278/140‑1 (MQ=255)                                                                                                         ctgcagcCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGAc                                        >  1:555553/1‑122 (MQ=255)                                                                                                         ctgcagcCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGAc                                        <  2:555553/122‑1 (MQ=255)                                                                                                              gcCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTg                 >  2:727198/1‑140 (MQ=255)                                                                                                                           gATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCATGGCGTCCACTTCGc    <  1:33619/140‑1 (MQ=255)                                                                                                                             tGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGccc  <  2:498504/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                               CGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCC  >  NC_000913/3177213‑3177476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑