Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,181,329	G→A	P303P (CCG→CCA)	ygiC →	putative enzyme with ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,181,329	0	G	A	100.0%	174.8 / NA	50	P303P (CCG→CCA)	ygiC	putative enzyme with ATPase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/26);  total (24/26)

CATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCT  >  NC_000913/3181206‑3181462                                                                                                                            |                                                                                                                                      cATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAg                                                                                                                       >  1:313531/1‑140 (MQ=255)  aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAgg                                                                                                                      <  2:123153/140‑1 (MQ=255)     aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGGAGgcgc                                                                                                                   >  1:464093/1‑140 (MQ=255)       ctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCaa                                                                                                                 <  1:666645/140‑1 (MQ=255)              aaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTc                                                                                                          <  2:658264/140‑1 (MQ=255)                ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAGTATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGa                                                                                                        >  2:743378/1‑140 (MQ=255)                ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGa                                                                                                        >  2:142160/1‑140 (MQ=255)                ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGa                                                                                                        >  2:28220/1‑140 (MQ=255)                ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGa                                                                                                        >  2:310367/1‑140 (MQ=255)                       cTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                                                                                                 >  1:303840/1‑140 (MQ=255)                             cTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACgg                                                                                           >  1:669437/1‑140 (MQ=255)                               aCTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCa                                                                                         <  2:864353/140‑1 (MQ=255)                                        gaTGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTg                                                                                <  2:303840/140‑1 (MQ=255)                                           gTTCCCGAATCACCCGAACCTGCTTCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag                                                                             >  2:6173/1‑140 (MQ=255)                                           gTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag                                                                             >  2:361785/1‑140 (MQ=255)                                            ttCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagc                                                                            <  2:313531/140‑1 (MQ=255)                                            ttCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagc                                                                            >  1:693879/1‑140 (MQ=255)                                                 gAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCgg                                                                       <  1:401420/140‑1 (MQ=255)                                                 gAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCgg                                                                       <  2:782625/140‑1 (MQ=255)                                                      aCCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGt                                                                  <  1:310367/140‑1 (MQ=255)                                                         cGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCg                                                               <  2:62865/140‑1 (MQ=255)                                                         cGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCg                                                               <  2:761572/140‑1 (MQ=255)                                                         cGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCg                                                               <  2:777642/140‑1 (MQ=255)                                                         cGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCg                                                               >  2:828956/1‑140 (MQ=255)                                                          gAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGt                                                              <  1:301497/140‑1 (MQ=255)                                                          gAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGt                                                              <  1:444291/140‑1 (MQ=255)                                                            aCCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTAt                                                            <  1:28220/140‑1 (MQ=255)                                                               tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAGTGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGaa                                                                     <  1:4188/128‑1 (MQ=255)                                                               tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAGTGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGaa                                                                     >  2:4188/1‑128 (MQ=255)                                                                    cccGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCgaaga                                                    <  2:149628/140‑1 (MQ=255)                                                                      cGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGaagaag                                                  >  2:685711/1‑140 (MQ=255)                                                                       gCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAgg                                                 <  1:142160/140‑1 (MQ=255)                                                                        cTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAggg                                                >  1:809862/1‑140 (MQ=255)                                                                           aTTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGgatg                                             >  1:196294/1‑140 (MQ=255)                                                                              ttGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGAtt                                          >  2:615225/1‑140 (MQ=255)                                                                                   gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCa                                     <  1:615225/140‑1 (MQ=255)                                                                                              tcCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGATCGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCAcc                          >  2:602694/1‑140 (MQ=255)                                                                                                cGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCa                                     <  2:352830/127‑1 (MQ=255)                                                                                                cGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCa                                     >  1:352830/1‑127 (MQ=255)                                                                                                cGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCg                        >  2:312939/1‑140 (MQ=255)                                                                                                                 gtgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       <  2:669437/140‑1 (MQ=255)                                                                                                                 gtgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       <  1:312939/140‑1 (MQ=255)                                                                                                                 gtgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       <  1:6173/140‑1 (MQ=255)                                                                                                                  tgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGc         <  1:34066/137‑1 (MQ=255)                                                                                                                  tgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGc         >  2:34066/1‑137 (MQ=255)                                                                                                                  tgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGAc      >  2:732425/1‑140 (MQ=255)                                                                                                                   ggTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACa     >  2:433918/1‑140 (MQ=255)                                                                                                                      tAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCt  <  1:685711/140‑1 (MQ=255)                                                                                                                        aaCCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       <  2:168950/133‑1 (MQ=255)                                                                                                                        aaCCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       >  1:168950/1‑133 (MQ=255)                                                                                                                            |                                                                                                                                      CATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCT  >  NC_000913/3181206‑3181462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑