Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,015	T→C	intergenic (‑50/‑192)	ygiF ← / → ygiM	inorganic triphosphatase/putative signal transduction protein (SH3 domain)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,015	0	T	C	100.0%	191.3 / NA	55	intergenic (‑50/‑192)	ygiF/ygiM	inorganic triphosphatase/putative signal transduction protein (SH3 domain)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

CTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTG  >  NC_000913/3200879‑3201147                                                                                                                                         |                                                                                                                                     cTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAAt                                                                                                                                   >  2:65996/1‑140 (MQ=255) cTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAAt                                                                                                                                   >  1:636520/1‑140 (MQ=255)   cgccgcCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGc                                                                                                                                 >  1:418281/1‑140 (MQ=255)    gccgccCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCg                                                                                                                                <  2:420867/140‑1 (MQ=255)     ccgccCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCAATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGa                                                                                                                               <  2:599476/140‑1 (MQ=255)         ccAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCtt                                                                                                                           >  1:23217/1‑140 (MQ=255)            gCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCttttt                                                                                                                        <  1:410896/140‑1 (MQ=255)              gTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTg                                                                                                                      <  1:312760/140‑1 (MQ=255)                 ttGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCa                                                                                                                   <  2:206685/140‑1 (MQ=255)                   gagaTGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGt                                                                                                                 <  2:503638/140‑1 (MQ=255)                     gaTGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAg                                                                                                               >  1:343218/1‑140 (MQ=255)                      aTGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGACCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGa                                                                                                              >  1:676672/1‑140 (MQ=255)                       tGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCa                                                                                                                   <  2:437510/134‑1 (MQ=255)                       tGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCa                                                                                                                   >  1:437510/1‑134 (MQ=255)                         gTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAg                                                                                                           >  2:698654/1‑140 (MQ=255)                           cACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTa                                                                                                         >  1:245582/1‑140 (MQ=255)                                  cGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTgcg                                                                                                  <  1:698654/140‑1 (MQ=255)                                    ccTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGcc                                                                                                >  1:832007/1‑140 (MQ=255)                                    ccTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGcc                                                                                                >  2:774166/1‑140 (MQ=255)                                                tgtgATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGc                                                                                    <  2:245582/140‑1 (MQ=255)                                                  tgATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCGCATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCaa                                                                                  <  2:676672/140‑1 (MQ=255)                                                  tgATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCaa                                                                                  <  1:774166/140‑1 (MQ=255)                                                  tgACTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCaa                                                                                  <  2:397183/140‑1 (MQ=255)                                                            tAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAAttt                                                                        >  1:705026/1‑140 (MQ=255)                                                            tAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAAttt                                                                        >  1:41859/1‑140 (MQ=255)                                                                cTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACg                                                                    <  2:705026/140‑1 (MQ=255)                                                                       tCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTaa                                                             <  2:832007/140‑1 (MQ=255)                                                                                  aGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGc                                                  >  1:449095/1‑140 (MQ=255)                                                                                       tGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTgg                                             >  2:729480/1‑140 (MQ=255)                                                                                         gCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCa                                           <  1:729480/140‑1 (MQ=255)                                                                                            tttACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGa                                        >  1:665746/1‑140 (MQ=255)                                                                                              tACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGAcc                                      >  2:570699/1‑140 (MQ=255)                                                                                                cTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTg                                    <  2:343218/140‑1 (MQ=255)                                                                                                   aTCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGaca                                 >  2:235746/1‑140 (MQ=255)                                                                                                   aTCGGTTATGTCACATAAATGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGAATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGAGAGTAAAAGTTTGAAGCAGTGGCAAGACCTGaca                                 >  2:121783/1‑140 (MQ=255)                                                                                                     cGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGAcaca                               <  2:295559/140‑1 (MQ=255)                                                                                                        ttATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACagag                            >  1:79999/1‑140 (MQ=255)                                                                                                        ttATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACagag                            <  2:733562/140‑1 (MQ=255)                                                                                                        ttATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACagag                            >  1:293054/1‑140 (MQ=255)                                                                                                          aTGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGa                          >  2:7193/1‑140 (MQ=255)                                                                                                            gTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGAtt                        <  2:418281/140‑1 (MQ=255)                                                                                                                caTAAGTGACGATGAGCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTGAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGa                    <  1:340715/140‑1 (MQ=255)                                                                                                                caTAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGa                    <  1:157863/140‑1 (MQ=255)                                                                                                                 aTAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGAt                   >  2:296378/1‑140 (MQ=255)                                                                                                                  tAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGAtt                  >  2:512449/1‑140 (MQ=255)                                                                                                                      tGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATg              >  1:613500/1‑140 (MQ=255)                                                                                                                      tGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATg              >  1:749898/1‑140 (MQ=255)                                                                                                                      tGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATg              >  1:797401/1‑140 (MQ=255)                                                                                                                        aCGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTc            >  1:396822/1‑140 (MQ=255)                                                                                                                          gATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGa          <  2:636520/140‑1 (MQ=255)                                                                                                                          gATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGa          <  2:449095/140‑1 (MQ=255)                                                                                                                          gATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGa          >  2:756553/1‑140 (MQ=255)                                                                                                                              aaCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGc      >  2:626830/1‑140 (MQ=255)                                                                                                                               aCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCt     <  2:665746/140‑1 (MQ=255)                                                                                                                               aCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCt     >  1:471621/1‑140 (MQ=255)                                                                                                                                  gCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTg  <  1:7193/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTG  >  NC_000913/3200879‑3201147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑