Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,307,825	G→A	G12G (GGC→GGT)	deaD ←	ATP‑dependent RNA helicase DeaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,307,825	0	G	A	100.0%	273.8 / NA	78	G12G (GGC→GGT)	deaD	ATP‑dependent RNA helicase DeaD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/42);  total (36/42)

CAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAG  >  NC_000913/3307689‑3307960                                                                                                                                         |                                                                                                                                        cAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGAccc                                                                                                                                      >  1:727615/1‑140 (MQ=255)   gTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAg                                                                                                                                    >  1:291881/1‑140 (MQ=255)                 cGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTgg                                                                                                                      >  2:36141/1‑140 (MQ=255)                 cGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTgg                                                                                                                      >  1:641657/1‑140 (MQ=255)                   tCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTCTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGtt                                                                                                                    <  2:788204/140‑1 (MQ=255)                             aCCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAg                                                                                                          <  2:223791/140‑1 (MQ=255)                             aCCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAg                                                                                                          >  2:439458/1‑140 (MQ=255)                             aCCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAg                                                                                                          <  1:800000/140‑1 (MQ=255)                             aCCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAg                                                                                                          >  2:800000/1‑140 (MQ=255)                             aCCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAg                                                                                                          >  1:708747/1‑140 (MQ=255)                              cccAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGc                                                                                                         <  2:727615/140‑1 (MQ=255)                              cccAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGc                                                                                                         <  2:641657/140‑1 (MQ=255)                               ccAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGcc                                                                                                        <  1:233316/140‑1 (MQ=255)                                cAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCa                                                                                                       <  1:188397/140‑1 (MQ=255)                                 aGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCAt                                                                                                      <  1:418895/140‑1 (MQ=255)                                   aaCGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGc                                                                                                         <  2:679528/135‑1 (MQ=255)                                   aaCGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGc                                                                                                         >  1:679528/1‑135 (MQ=255)                                        cgcgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGcc                                                                                                        <  2:153461/131‑1 (MQ=255)                                        cgcgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTAc                                                                                               >  2:822047/1‑140 (MQ=255)                                        cgcgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTAc                                                                                               >  2:702561/1‑140 (MQ=255)                                        cgcgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGAACCAGATCTGCAAAAGTGGTTTCGAATTCAGcc                                                                                                        >  1:153461/1‑131 (MQ=255)                                          cgGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACgt                                                                                             >  1:426755/1‑140 (MQ=255)                                                 tCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTc                                                                                      >  1:756950/1‑140 (MQ=255)                                                             gAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACgtg                                                                                            >  2:572194/1‑122 (MQ=255)                                                             gAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACgtg                                                                                            <  1:572194/122‑1 (MQ=255)                                                               aTACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATggcgg                                                                        <  2:708747/140‑1 (MQ=255)                                                               aTACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATggcgg                                                                        <  2:91638/140‑1 (MQ=255)                                                                tACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATggcggc                                                                       <  2:41985/140‑1 (MQ=255)                                                                tACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATggcggc                                                                       <  1:766101/140‑1 (MQ=255)                                                                 acacTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGcc                                                                      <  2:756628/140‑1 (MQ=255)                                                                  cacTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCa                                                                     <  2:291881/140‑1 (MQ=255)                                                                  cacTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCa                                                                     >  2:393056/1‑140 (MQ=255)                                                                   acTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAg                                                                    >  1:743995/1‑140 (MQ=255)                                                                   acTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAg                                                                    >  2:539240/1‑140 (MQ=255)                                                                    ctctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGGATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   <  2:174645/140‑1 (MQ=255)                                                                    ctctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   <  2:199378/140‑1 (MQ=255)                                                                    ctctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   <  1:439458/140‑1 (MQ=255)                                                                    ctctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   >  2:326270/1‑140 (MQ=255)                                                                      ctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCaat                                                                   >  1:54729/1‑136 (MQ=255)                                                                      ctGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   <  2:54729/138‑1 (MQ=255)                                                                       tGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATggcgg                                                                        >  2:438671/1‑132 (MQ=255)                                                                       tGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATggcgg                                                                        <  1:438671/132‑1 (MQ=255)                                                                       tGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   >  1:162460/1‑137 (MQ=255)                                                                       tGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   <  1:345248/137‑1 (MQ=255)                                                                       tGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   <  2:162460/137‑1 (MQ=255)                                                                       tGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   >  2:345248/1‑137 (MQ=255)                                                                       tGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTa                                                                >  1:242361/1‑140 (MQ=255)                                                                         ccTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACa                                                              <  1:702561/140‑1 (MQ=255)                                                                            gAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATaa                                                           <  1:393056/140‑1 (MQ=255)                                                                             aaTTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   <  2:242228/131‑1 (MQ=255)                                                                             aaTTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGt                                                                   >  1:242228/1‑131 (MQ=255)                                                                              aTTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACt                                                         <  1:326270/140‑1 (MQ=255)                                                                                 ggAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACtcat                                                      >  2:116309/1‑140 (MQ=255)                                                                                   agaTGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACtcatca                                                    >  2:588547/1‑140 (MQ=255)                                                                                      tGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGa                                                 <  1:539240/140‑1 (MQ=255)                                                                                            ttCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGa                                           >  2:562773/1‑140 (MQ=255)                                                                                                           gTTAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCAtt                            <  1:489206/140‑1 (MQ=255)                                                                                                             tAAGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGa                          >  2:752587/1‑140 (MQ=255)                                                                                                              aaGGGCTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGaa                         <  1:241334/140‑1 (MQ=255)                                                                                                                   cTTCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAgtg                    >  1:127555/1‑140 (MQ=255)                                                                                                                    ttCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAgtgt                   >  2:555733/1‑140 (MQ=255)                                                                                                                     tCAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGtgtg                  >  2:204090/1‑140 (MQ=255)                                                                                                                      cAAGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGa                 >  1:85743/1‑140 (MQ=255)                                                                                                                       aaGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGaa                <  1:736621/140‑1 (MQ=255)                                                                                                                        aGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAAc               <  1:204090/140‑1 (MQ=255)                                                                                                                        aGGATAGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAAc               <  2:127555/140‑1 (MQ=255)                                                                                                                             aGGAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCt          <  2:242361/140‑1 (MQ=255)                                                                                                                               gAGCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCa        >  2:688784/1‑140 (MQ=255)                                                                                                                                 gCCTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCaaa      <  1:116309/140‑1 (MQ=255)                                                                                                                                  ccTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAg     <  2:426755/140‑1 (MQ=255)                                                                                                                                   cTTCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGt    <  1:688784/140‑1 (MQ=255)                                                                                                                                     tCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCgg            <  2:626571/130‑1 (MQ=255)                                                                                                                                     tCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCgg            <  2:171150/130‑1 (MQ=255)                                                                                                                                     tCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCgg            <  2:765498/130‑1 (MQ=255)                                                                                                                                     tCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCgg            >  1:171150/1‑130 (MQ=255)                                                                                                                                     tCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCgg            >  1:765498/1‑130 (MQ=255)                                                                                                                                     tCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCgg            >  1:626571/1‑130 (MQ=255)                                                                                                                                     tCAGACCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAg  <  2:85743/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAG  >  NC_000913/3307689‑3307960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑