Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,368,107	A→G	Y94C (TAC→TGC)	yhcG →	DUF1016 domain‑containing protein YhcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,368,107	0	A	G	100.0%	123.7 / NA	36	Y94C (TAC→TGC)	yhcG	DUF1016 domain‑containing protein YhcG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

ATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCC  >  NC_000913/3367971‑3368240                                                                                                                                         |                                                                                                                                      atggTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCct                                                                                                                                    <  2:531522/137‑1 (MQ=255) aTTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCct                                                                                                                                    <  1:655048/140‑1 (MQ=255)         aTTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTc                                                                                                                            <  1:5755/140‑1 (MQ=255)          tttGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCa                                                                                                                           >  1:335080/1‑140 (MQ=255)                       gCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAAtt                                                                                                              <  2:243914/140‑1 (MQ=255)                          aGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCAc                                                                                                           <  1:676589/140‑1 (MQ=255)                             ccAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAg                                                                                                        >  1:155615/1‑140 (MQ=255)                                 ggCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACaa                                                                                                    >  1:423913/1‑140 (MQ=255)                                      cGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCt                                                                                               >  2:170685/1‑140 (MQ=255)                                        tATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTgg                                                                                             <  2:423913/140‑1 (MQ=255)                                         aTGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGc                                                                                            <  2:155615/140‑1 (MQ=255)                                           ggTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGa                                                                                          >  2:744062/1‑140 (MQ=255)                                             tGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAAt                                                                                        <  2:33583/140‑1 (MQ=255)                                                gcAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGa                                                                                                       >  2:813674/1‑122 (MQ=255)                                                gcAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGa                                                                                                       <  1:813674/122‑1 (MQ=255)                                                gcAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTaa                                                                                     >  2:159480/1‑140 (MQ=255)                                                gcAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTaa                                                                                     <  1:499414/140‑1 (MQ=255)                                                  aGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAaca                                                                                   >  2:480776/1‑140 (MQ=255)                                                            gCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGaa                                                                         <  1:170685/140‑1 (MQ=255)                                                             cGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAAt                                                                        <  1:159480/140‑1 (MQ=255)                                                                          aTTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCg                                                           >  2:123688/1‑140 (MQ=255)                                                                             tATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCt                                                        >  1:630904/1‑140 (MQ=255)                                                                             tATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCt                                                        >  1:242121/1‑140 (MQ=255)                                                                                          taAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCAt                                           >  2:351257/1‑140 (MQ=255)                                                                                                 gggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaaa                                    >  1:224451/1‑140 (MQ=255)                                                                                                 gggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaaa                                    >  1:711154/1‑140 (MQ=255)                                                                                                  ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAg                                   >  2:574653/1‑140 (MQ=255)                                                                                                  ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAg                                   >  2:824301/1‑140 (MQ=255)                                                                                                   gTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGa                                  <  1:480776/140‑1 (MQ=255)                                                                                                    ttCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAt                                 <  2:711154/140‑1 (MQ=255)                                                                                                         tGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGc                            <  2:224451/140‑1 (MQ=255)                                                                                                               aaaCTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGc                      <  1:351257/140‑1 (MQ=255)                                                                                                                aaCTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCt                     <  1:123688/140‑1 (MQ=255)                                                                                                                         cAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaa            <  2:88258/140‑1 (MQ=255)                                                                                                                                   ttttttGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcccc  <  2:242121/140‑1 (MQ=255)                                                                                                                                   ttttttGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcccc  <  2:630904/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      ATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCC  >  NC_000913/3367971‑3368240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑