Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,603,550	Δ1 bp	coding (1733/2019 nt)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,603,547	0	C	.	100.0%	316.8 / NA	75	coding (1730/2019 nt)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (32/43);  total (32/43)

CATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACA  >  NC_000913/2603412‑2603680                                                                                                                                        |                                                                                                                                      cATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGcccgt                                                                                                                                   >  1:10032/1‑138 (MQ=255) cATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGcccgt                                                                                                                                   >  2:573461/1‑138 (MQ=255) cATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGcccgt                                                                                                                                   >  1:1065622/1‑138 (MQ=255) cATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGcccgt                                                                                                                                   >  1:195422/1‑138 (MQ=255)  aTTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGcccgtc                                                                                                                                  <  2:149676/140‑4 (MQ=255)         ttGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAAt                                                                                                                          >  2:554353/1‑140 (MQ=255)         ttGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAAt                                                                                                                          >  2:35398/1‑140 (MQ=255)           gCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATgg                                                                                                                        >  1:1070441/1‑140 (MQ=255)            cTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGc                                                                                                                       >  1:992913/1‑140 (MQ=255)              gTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGt                                                                                                                     <  2:1191117/140‑1 (MQ=255)              gTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGt                                                                                                                     <  2:1033360/140‑1 (MQ=255)              gTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGt                                                                                                                     <  1:493414/140‑1 (MQ=255)              gTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGt                                                                                                                     <  2:1070441/140‑1 (MQ=255)                tactacTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGa                                                                                                                   <  2:1165414/140‑1 (MQ=255)                  ctactGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATg                                                                                                                 <  1:159878/140‑1 (MQ=255)                   tactGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGc                                                                                                                >  1:933940/1‑140 (MQ=255)                     ctGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAg                                                                                                              >  2:499068/1‑140 (MQ=255)                        gCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCg                                                                                                           <  2:41725/140‑1 (MQ=255)                        gCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCg                                                                                                           >  1:365428/1‑140 (MQ=255)                        gCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTGCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGGATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCg                                                                                                           <  2:19184/140‑1 (MQ=255)                          gATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCt                                                                                                         <  2:65061/140‑1 (MQ=255)                          gATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCt                                                                                                         <  1:35398/140‑1 (MQ=255)                           aTGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTg                                                                                                        >  2:1099019/1‑140 (MQ=255)                            tGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGc                                                                                                       >  1:58618/1‑140 (MQ=255)                            tGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGc                                                                                                       >  2:922078/1‑140 (MQ=255)                                   gctgcCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGt                                                                                                <  2:1189854/140‑1 (MQ=255)                                      gcCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCtt                                                                                             >  1:887340/1‑140 (MQ=255)                                      gcCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCtt                                                                                             >  1:614988/1‑140 (MQ=255)                                        cTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCtttt                                                                                           >  1:772483/1‑140 (MQ=255)                                            ccTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTgc                                                                                       <  2:1005849/140‑1 (MQ=255)                                             cTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTgcg                                                                                      <  1:753975/140‑1 (MQ=255)                                                  cTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTAtt                                                                                 <  2:887340/140‑1 (MQ=255)                                                     gCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCg                                                                              <  1:1041903/140‑1 (MQ=255)                                                     gCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCg                                                                              >  2:1135919/1‑140 (MQ=255)                                                     gCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCg                                                                              <  2:58618/140‑1 (MQ=255)                                                         tggtgTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTa                                                                          <  2:195422/140‑1 (MQ=255)                                                          ggtgTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCg                                                                              >  2:807031/1‑135 (MQ=255)                                                          ggtgTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCg                                                                              <  1:807031/135‑1 (MQ=255)                                                          ggtgTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTAc                                                                         >  2:484530/1‑140 (MQ=255)                                                               cGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGacaa                                                                    <  2:9880/140‑1 (MQ=255)                                                               cGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGacaa                                                                    <  2:933940/140‑1 (MQ=255)                                                               cGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGacaa                                                                    <  2:614988/140‑1 (MQ=255)                                                                 ttCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCCGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGacaaca                                                                  >  1:1151978/1‑140 (MQ=255)                                                                 ttCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGacaaca                                                                  >  2:971168/1‑140 (MQ=255)                                                                    gcgccgcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCt                                                               <  1:444381/140‑1 (MQ=255)                                                                    gcgccgcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCt                                                               <  1:132594/140‑1 (MQ=255)                                                                     cgccgcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTc                                                              >  2:616849/1‑140 (MQ=255)                                                                      gccgcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCCGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCg                                                             <  2:1151978/140‑1 (MQ=255)                                                                      gccgcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGacaa                                                                    >  1:637457/1‑133 (MQ=255)                                                                      gccgcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGacaa                                                                    <  2:637457/133‑1 (MQ=255)                                                                       ccgcgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGa                                                            <  1:1135919/140‑1 (MQ=255)                                                                          cgcAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGAccc                                                         >  2:898919/1‑140 (MQ=255)                                                                               cGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGc                                                    <  1:554353/140‑1 (MQ=255)                                                                                    cgtcgCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAgg                                               <  1:486202/140‑1 (MQ=255)                                                                                    cgtcgCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAgg                                               <  2:10032/140‑1 (MQ=255)                                                                                           caGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCtaaata                                        <  2:772483/140‑1 (MQ=255)                                                                                            aGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCtaaataa                                       <  1:679978/140‑1 (MQ=255)                                                                                                 aTGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTc                                  >  2:826651/1‑140 (MQ=255)                                                                                                    ccTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTg                               >  1:448516/1‑140 (MQ=255)                                                                                                     cTGGGCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTgc                              >  2:1163666/1‑140 (MQ=255)                                                                                                       gggCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTgcgc                            <  1:1099019/140‑1 (MQ=255)                                                                                                       gggCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTgcgc                            <  2:448516/140‑1 (MQ=255)                                                                                                        ggCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCa                           >  1:577860/1‑140 (MQ=255)                                                                                                         gCATGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCAc                          <  2:1085802/140‑1 (MQ=255)                                                                                                           aTGCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGt                        <  1:484530/140‑1 (MQ=255)                                                                                                             gCGGCTACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCa                      >  2:845112/1‑140 (MQ=255)                                                                                                                  tACGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGcc                 <  2:577860/140‑1 (MQ=255)                                                                                                                   aCGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCa                >  1:444867/1‑140 (MQ=255)                                                                                                                   aCGGCTGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCa                <  1:616849/140‑1 (MQ=255)                                                                                                                        tGGGAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCt           <  1:898919/140‑1 (MQ=255)                                                                                                                          ggAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCa         <  2:444867/140‑1 (MQ=255)                                                                                                                          ggAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCa         >  2:42707/1‑140 (MQ=255)                                                                                                                          ggAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCa         >  2:899151/1‑140 (MQ=255)                                                                                                                          ggAAAATGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCa         <  1:971168/140‑1 (MQ=255)                                                                                                                            aaaaTGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCaga       <  2:159161/140‑1 (MQ=255)                                                                                                                               aTGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGca    >  1:967172/1‑140 (MQ=255)                                                                                                                               aTGCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGca    >  1:1030359/1‑140 (MQ=255)                                                                                                                                 gCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGcaca  <  1:826651/140‑1 (MQ=255)                                                                                                                                 gCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTCCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCagag      <  1:954253/136‑1 (MQ=255)                                                                                                                                 gCGATGG‑CCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTCCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCagag      >  2:954253/1‑136 (MQ=255)                                                                                                                                        |                                                                                                                                      CATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGTCGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGAAAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGCCGCTGCGTGTGGTCTTTTCTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTAAATAAAGGTCTTGCGCACGTCACCGCCAGGGCTCAGAGCACA  >  NC_000913/2603412‑2603680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑