Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,826,581	Δ1 bp	coding (190/960 nt)	srlE →	sorbitol‑specific PTS enzyme IIBC1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,826,579	0	A	.	100.0%	290.9 / NA	69	coding (188/960 nt)	srlE	sorbitol‑specific PTS enzyme IIBC1 component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (32/37);  total (32/37)

CTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGA  >  NC_000913/2826440‑2826708                                                                                                                                            |                                                                                                                                  cTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGa                                                                                                                                   >  2:448371/1‑140 (MQ=255)   tGAGCTAAACGCCACGCGGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGaat                                                                                                                                 <  2:80684/140‑2 (MQ=255)       cTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGgt                                                                                                                            >  1:14494/1‑140 (MQ=255)         gAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGgtgt                                                                                                                          <  1:386471/140‑1 (MQ=255)               cACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGt                                                                                                                    >  1:428511/1‑140 (MQ=255)                 cGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTaa                                                                                                                  >  2:425189/1‑140 (MQ=255)                    cGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCg                                                                                                               <  2:650768/140‑1 (MQ=255)                      gaaaaaaaaaTCGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGAc                                                                                                             <  2:169622/138‑1 (MQ=255)                       gAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACt                                                                                                            >  2:1190560/1‑140 (MQ=255)                         aaaaaaaTCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACtgt                                                                                                          <  1:1054942/140‑1 (MQ=255)                             aaaTCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTggcg                                                                                                      <  1:627658/140‑1 (MQ=255)                             aaaTCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTggcg                                                                                                      <  1:177251/140‑1 (MQ=255)                             aaaTCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTggcg                                                                                                      <  2:934928/140‑1 (MQ=255)                             aaaTCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTggcg                                                                                                      <  2:14494/140‑1 (MQ=255)                                  gTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGcaca                                                                                                 >  1:568373/1‑140 (MQ=255)                                      atatCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTAc                                                                                             <  2:568373/140‑1 (MQ=255)                                              gccgGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGGACCCGCGGAAGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGc                                                                                     >  2:1087681/1‑140 (MQ=255)                                               ccgGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCa                                                                                    >  1:253606/1‑140 (MQ=255)                                                cgGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCAt                                                                                   <  2:1011516/140‑1 (MQ=255)                                                cgGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCAt                                                                                   <  2:362482/140‑1 (MQ=255)                                                 ggTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATc                                                                                  >  2:477705/1‑140 (MQ=255)                                                  gTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTAc                                                                                             <  2:287198/128‑1 (MQ=255)                                                  gTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTAc                                                                                             >  1:502289/1‑128 (MQ=255)                                                  gTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTAc                                                                                             <  2:502289/128‑1 (MQ=255)                                                  gTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTAc                                                                                             >  1:287198/1‑128 (MQ=255)                                                     cccGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATc                                                                              >  2:228954/1‑140 (MQ=255)                                                       cGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCg                                                                            >  2:953567/1‑140 (MQ=255)                                                          ccTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaaa                                                                         >  2:192239/1‑140 (MQ=255)                                                          ccTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaaa                                                                         >  1:206311/1‑140 (MQ=255)                                                          ccTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaa                                                                          <  1:1168696/139‑1 (MQ=255)                                                          ccTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaa                                                                          >  2:1168696/1‑139 (MQ=255)                                                             gCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAacga                                                                      <  1:192239/140‑1 (MQ=255)                                                               gATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAacgacg                                                                    <  1:763289/140‑1 (MQ=255)                                                                 ttgttgACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTa                                                                  <  1:477705/140‑1 (MQ=255)                                                                    ttgACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTc                                                               >  2:668151/1‑140 (MQ=255)                                                                     tgACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                                              <  1:668151/140‑1 (MQ=255)                                                                     tgACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                                              <  1:401438/140‑1 (MQ=255)                                                                        cAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCccac                                                           <  1:674632/140‑1 (MQ=255)                                                                        cAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCcca                                                            <  1:1080137/139‑1 (MQ=255)                                                                        cAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCcca                                                            >  2:1080137/1‑139 (MQ=255)                                                                              ggCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCAACTATCCGAAACGACGTATTCCCACCATGaa                                                     >  2:745407/1‑140 (MQ=255)                                                                               gCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAat                                                    <  2:428511/140‑1 (MQ=255)                                                                                     cTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCAc                                              >  1:302415/1‑140 (MQ=255)                                                                                      ttACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCa                                               <  1:17705/138‑1 (MQ=255)                                                                                      ttACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCa                                               >  2:17705/1‑138 (MQ=255)                                                                                      ttACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACt                                             >  2:521590/1‑140 (MQ=255)                                                                                              tggcAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGc                                     <  1:448371/140‑1 (MQ=255)                                                                                               ggcAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCa                                    >  1:943580/1‑140 (MQ=255)                                                                                                gcAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCaa                                   >  1:966438/1‑140 (MQ=255)                                                                                                gcAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCaa                                   >  2:178967/1‑140 (MQ=255)                                                                                                  aGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAgt                                 >  1:985051/1‑140 (MQ=255)                                                                                                      tATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAgtccgg                             >  1:361346/1‑140 (MQ=255)                                                                                                        ttGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAgtccggtc                           <  1:811044/140‑1 (MQ=255)                                                                                                             ggATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTg                      <  1:228954/140‑1 (MQ=255)                                                                                                              gATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTg                      >  2:1208984/1‑139 (MQ=255)                                                                                                              gATTTAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTg                      <  1:1208984/139‑1 (MQ=255)                                                                                                                  tAAAGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCa                 <  2:985051/140‑1 (MQ=255)                                                                                                                   aaaGAAGGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAg                <  1:941341/140‑1 (MQ=255)                                                                                                                       aagGTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACa            <  1:51316/140‑1 (MQ=255)                                                                                                                         ggTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGgc                    <  2:1174742/130‑1 (MQ=255)                                                                                                                         ggTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGgc                    >  1:1174742/1‑130 (MQ=255)                                                                                                                         ggTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACAtt          >  2:830044/1‑140 (MQ=255)                                                                                                                         ggTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACAtt          >  2:184851/1‑140 (MQ=255)                                                                                                                          gTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTgg                     >  1:822996/1‑128 (MQ=255)                                                                                                                          gTGAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTgg                     <  2:822996/128‑1 (MQ=255)                                                                                                                            gAACCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATtgtg       <  2:966438/140‑1 (MQ=255)                                                                                                                              aCCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGa     <  1:521590/140‑1 (MQ=255)                                                                                                                              aCCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGa     <  1:830044/140‑1 (MQ=255)                                                                                                                              aCCCGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGa     <  1:1087681/140‑1 (MQ=255)                                                                                                                                ccGCGGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAg   <  1:1190560/140‑1 (MQ=255)                                                                                                                                 cgcgGAGGCGG‑AATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGa  >  2:567821/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                  CTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGA  >  NC_000913/2826440‑2826708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑