Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,042,391	Δ1 bp	intergenic (+98/+98)	ygfZ → / ← yqfA	folate‑binding protein/hemolysin‑III family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,042,389	0	C	.	100.0%	204.2 / NA	49	intergenic (+96/+100)	ygfZ/yqfA	folate‑binding protein/hemolysin‑III family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (28/21);  total (28/21)

GCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAA  >  NC_000913/3042257‑3042523                                                                                                                                     |                                                                                                                                       gCATATCGAGCCGCTGTCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGAt                                                                                                                                >  2:195760/1‑140 (MQ=255)  cATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATc                                                                                                                               >  2:1132925/1‑140 (MQ=255)      tCGAGCCGCTGCCGTATTCGCTCGAAGAGTGAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGAt                                                                                                                           <  2:86525/140‑1 (MQ=255)                           tCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCg                                                                                                      >  2:226964/1‑140 (MQ=255)                              aaGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCa                                                                                                   >  2:321690/1‑140 (MQ=255)                                    aaaTCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTtgtg                                                                                             <  1:195760/140‑1 (MQ=255)                                         tctTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCg                                                                                        <  2:569456/140‑1 (MQ=255)                                                            ttATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTgg                                                                     >  1:55756/1‑140 (MQ=255)                                                             tATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc                                                                    >  2:1129025/1‑140 (MQ=255)                                                                 gCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGt                                                                <  1:612137/140‑1 (MQ=255)                                                                 gCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGt                                                                <  1:382173/140‑1 (MQ=255)                                                                  ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc                                                                    <  1:532207/135‑1 (MQ=255)                                                                  ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc                                                                    >  2:532207/1‑135 (MQ=255)                                                                  ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                               >  1:624204/1‑140 (MQ=255)                                                                  ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                               >  2:1152202/1‑140 (MQ=255)                                                                  ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                               >  1:1165775/1‑140 (MQ=255)                                                                  ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCAAGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                               >  1:969155/1‑140 (MQ=255)                                                                   cTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCt                                                              >  2:739949/1‑140 (MQ=255)                                                                    tGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCta                                                             >  1:731432/1‑139 (MQ=255)                                                                           aCGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                      >  1:242894/1‑140 (MQ=255)                                                                                ggCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                                                 <  1:226964/140‑1 (MQ=255)                                                                                ggCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                                                 >  2:118140/1‑140 (MQ=255)                                                                                 gcgcGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCtt                                                             >  2:797800/1‑127 (MQ=255)                                                                                 gcgcGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCtt                                                             <  1:797800/127‑1 (MQ=255)                                                                                 gcgcGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaa                                                <  2:624204/140‑1 (MQ=255)                                                                                    cgTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAgg                                             <  1:648324/140‑1 (MQ=255)                                                                                      tCTTATCATGTCTGGGCTTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                                                 >  2:844020/1‑134 (MQ=255)                                                                                      tCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                                                 <  1:844020/134‑1 (MQ=255)                                                                                                tCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTAc                                 >  1:592206/1‑140 (MQ=255)                                                                                                    ggATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCt                             <  2:592206/140‑1 (MQ=255)                                                                                                    ggATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCt                             >  1:587210/1‑140 (MQ=255)                                                                                                    ggATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCt                             <  1:118140/140‑1 (MQ=255)                                                                                                       tttGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGcc                          <  2:977111/140‑1 (MQ=255)                                                                                                       tttGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGcc                          <  1:1132925/140‑1 (MQ=255)                                                                                                        ttGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGccc                         <  1:1132267/140‑1 (MQ=255)                                                                                                          gATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCAt                                    <  1:521894/127‑1 (MQ=255)                                                                                                          gATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCAt                                    >  2:521894/1‑127 (MQ=255)                                                                                                          gATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCaa                       <  2:1165775/140‑1 (MQ=255)                                                                                                          gATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCaa                       <  1:519666/140‑1 (MQ=255)                                                                                                            tGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAata                     <  1:321690/140‑1 (MQ=255)                                                                                                              ccTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAAtata                   >  2:1032673/1‑140 (MQ=255)                                                                                                               cTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATAc                  >  1:1007633/1‑140 (MQ=255)                                                                                                                   aTCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAAt              >  1:391507/1‑140 (MQ=255)                                                                                                                      acaacaGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGa           <  1:1129025/140‑1 (MQ=255)                                                                                                                             ggACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAg    >  1:1067737/1‑140 (MQ=255)                                                                                                                             ggACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAg    >  2:936685/1‑140 (MQ=255)                                                                                                                             ggACGAGG‑CCGCAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAg    >  1:34230/1‑140 (MQ=255)                                                                                                                               aCGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGaa  >  1:623469/1‑140 (MQ=255)                                                                                                                               aCGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGaa  >  1:552793/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       GCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAA  >  NC_000913/3042257‑3042523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑