Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,181,068	(A)5→4	coding (648/1161 nt)	ygiC →	putative enzyme with ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,181,064	0	A	.	100.0%	356.1 / NA	84	coding (644/1161 nt)	ygiC	putative enzyme with ATPase activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (44/40);  total (44/40)

ATGGCTTCCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTG  >  NC_000913/3180926‑3181199                                                                                                                                           |                                                                                                                                        aTGGCTTCCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaa                                                                                                                                        >  1:539616/1‑140 (MQ=255) aTGGCTTCCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaa                                                                                                                                        >  1:602487/1‑140 (MQ=255)      ttCCAGTTGCTGCATCTCACCTTCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaaaaggt                                                                                                                                   <  1:858068/140‑4 (MQ=255)      ttCCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaaaaggt                                                                                                                                   <  2:943228/140‑4 (MQ=255)         cAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaaaaggt                                                                                                                                   >  1:1200408/1‑134 (MQ=255)         cAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaaaaggt                                                                                                                                   <  2:1200408/137‑4 (MQ=255)              gctgcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCAc                                                                                                                          <  1:833287/140‑1 (MQ=255)               ctgcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACg                                                                                                                         <  2:870202/140‑1 (MQ=255)               ctgcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACg                                                                                                                         <  2:602487/140‑1 (MQ=255)                tgcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACgg                                                                                                                        >  1:87266/1‑140 (MQ=255)                 gcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGa                                                                                                                       <  2:87266/140‑1 (MQ=255)                        aCCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAg                                                                                                                >  2:1146010/1‑140 (MQ=255)                           tgctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGAt                                                                                                             <  1:727783/140‑1 (MQ=255)                           tgctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGAt                                                                                                             <  2:539616/140‑1 (MQ=255)                             ctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTa                                                                                                                   <  2:316699/132‑1 (MQ=255)                             ctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTa                                                                                                                   >  1:316699/1‑132 (MQ=255)                             ctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCa                                                                                                           >  1:639817/1‑140 (MQ=255)                             ctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCa                                                                                                           >  2:587856/1‑140 (MQ=255)                               gTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAgg                                                                                                         >  1:513045/1‑140 (MQ=255)                                tCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGt                                                                                                        <  1:81052/140‑1 (MQ=255)                                  gcgACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTaa                                                                                                      >  1:856857/1‑140 (MQ=255)                                       acGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTcc                                                                                                 >  1:871832/1‑140 (MQ=255)                                          gTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAAc                                                                                              <  1:587856/140‑1 (MQ=255)                                          gTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAAc                                                                                              >  1:154572/1‑140 (MQ=255)                                               aGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGtt                                                                                         <  2:871832/140‑1 (MQ=255)                                                 aTCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCa                                                                                       <  1:326359/140‑1 (MQ=255)                                                   cgcgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAAcc                                                                                             >  1:930653/1‑132 (MQ=255)                                                   cgcgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAAcc                                                                                             <  2:930653/132‑1 (MQ=255)                                                   cgcgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCaaa                                                                                     >  2:885635/1‑140 (MQ=255)                                                   cgcgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCaaa                                                                                     <  2:936251/140‑1 (MQ=255)                                                    gcgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAAc                                                                                    >  2:1024913/1‑140 (MQ=255)                                                     cgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACt                                                                                   >  1:629608/1‑140 (MQ=255)                                                     cgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACt                                                                                   >  1:34769/1‑140 (MQ=255)                                                     cgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACt                                                                                   >  1:1093406/1‑140 (MQ=255)                                                     cgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACt                                                                                   >  2:674981/1‑140 (MQ=255)                                                       gAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGt                                                                                 >  2:852388/1‑140 (MQ=255)                                                       gAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGt                                                                                 >  1:704521/1‑140 (MQ=255)                                                        aaCCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTa                                                                                <  2:513045/140‑1 (MQ=255)                                                        aaCCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTa                                                                                <  2:301226/140‑1 (MQ=255)                                                          ccATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATc                                                                              >  1:937949/1‑140 (MQ=255)                                                             ttCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGt                                                                           <  1:885635/140‑1 (MQ=255)                                                                       gCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTAt                                                                 >  1:724987/1‑140 (MQ=255)                                                                          ggACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGtt                                                              >  2:1110863/1‑140 (MQ=255)                                                                              tGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGt                                                          >  2:1067011/1‑140 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATg                                                                >  1:641326/1‑132 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATg                                                                <  2:641326/132‑1 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGc                                                            <  1:1212739/136‑1 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGc                                                            >  2:1212739/1‑136 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTga                                                        >  1:302215/1‑140 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTga                                                        >  2:539340/1‑140 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTga                                                        >  2:960177/1‑140 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTga                                                        >  1:319898/1‑140 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTga                                                        >  1:438794/1‑140 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTga                                                        >  1:538794/1‑140 (MQ=255)                                                                                cgcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTga                                                        >  1:930216/1‑140 (MQ=255)                                                                                 gcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTgag                                                       <  1:1146010/140‑1 (MQ=255)                                                                                 gcAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTgag                                                       <  1:1110863/140‑1 (MQ=255)                                                                                     cGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGt                                                   >  2:131421/1‑140 (MQ=255)                                                                                      ggAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGtt                                                  <  1:283429/140‑1 (MQ=255)                                                                                          gCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATg                                                                <  2:667599/122‑1 (MQ=255)                                                                                          gCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATg                                                                >  1:667599/1‑122 (MQ=255)                                                                                            tGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAAc                                            <  2:814220/140‑1 (MQ=255)                                                                                            tGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAAc                                            <  2:438794/140‑1 (MQ=255)                                                                                                  tGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCt                                      >  1:976540/1‑140 (MQ=255)                                                                                                   gCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTg                                     <  2:679693/140‑1 (MQ=255)                                                                                                   gCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTg                                     <  2:319898/140‑1 (MQ=255)                                                                                                   gCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTg                                     <  2:639817/140‑1 (MQ=255)                                                                                                     tACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTgga                                   >  1:105385/1‑140 (MQ=255)                                                                                                     tACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTgga                                   <  2:34769/140‑1 (MQ=255)                                                                                                         gAGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGAt                               <  1:131421/140‑1 (MQ=255)                                                                                                          aGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATg                              <  2:538794/140‑1 (MQ=255)                                                                                                          aGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATg                              <  2:470392/140‑1 (MQ=255)                                                                                                          aGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATg                              <  2:1093406/140‑1 (MQ=255)                                                                                                          aGTTCCTCTACATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATg                              <  2:538943/140‑1 (MQ=255)                                                                                                                    cATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACg                    <  1:1024913/140‑1 (MQ=255)                                                                                                                    cATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACg                    <  2:856857/140‑1 (MQ=255)                                                                                                                    cATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACg                    <  1:852388/140‑1 (MQ=255)                                                                                                                    cATCGATGATATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACg                    <  2:937949/140‑1 (MQ=255)                                                                                                                         atgatATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACgctggc               >  2:218565/1‑140 (MQ=255)                                                                                                                         atgatATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACgctggc               >  2:378958/1‑140 (MQ=255)                                                                                                                         atgatATCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACgctggc               >  2:939686/1‑140 (MQ=255)                                                                                                                             tatCGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGa           >  2:204806/1‑140 (MQ=255)                                                                                                                                cGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAAcc        >  2:858091/1‑140 (MQ=255)                                                                                                                                cGGGTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAAcc        >  1:1109268/1‑140 (MQ=255)                                                                                                                                   gTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACg                    <  2:351159/125‑1 (MQ=255)                                                                                                                                   gTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACg                    >  1:351159/1‑125 (MQ=255)                                                                                                                                   gTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGc     <  1:939686/140‑1 (MQ=255)                                                                                                                                   gTTAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGc     >  1:631566/1‑140 (MQ=255)                                                                                                                                    ttAGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCg    <  1:1047271/140‑1 (MQ=255)                                                                                                                                      aGGTG‑AAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTg  >  1:525524/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        ATGGCTTCCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTG  >  NC_000913/3180926‑3181199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑